Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	714,075	T→G	V173G (GTG→GGG)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	714,075	0	T	G	100.0%	222.0 / NA	57	V173G (GTG→GGG)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/23);  total (34/23)

CAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGTGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGATATCGT  >  NC_000913/713929‑714213                                                                                                                                                   |                                                                                                                                           cAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGaa                                                                                                                                        <  1:567186/151‑1 (MQ=255) cAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGa                                                                                                                                         >  2:84427/1‑150 (MQ=255)    tGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGa                                                                                                                                      >  2:150564/1‑150 (MQ=255)           cTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGcc                                                                                                                               >  1:302415/1‑150 (MQ=255)            tGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCa                                                                                                                              >  2:529498/1‑150 (MQ=255)                   caATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCActg                                                                                                                      >  2:541305/1‑151 (MQ=255)                        aaaaaGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGc                                                                                                                 <  2:434171/151‑1 (MQ=255)                          aaaGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCg                                                                                                               >  2:356186/1‑151 (MQ=255)                              gTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATgg                                                                                                           <  1:374296/151‑1 (MQ=255)                                 gCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCt                                                                                                        <  1:468762/151‑1 (MQ=255)                                    cGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGa                                                                                                      >  2:613343/1‑150 (MQ=255)                                    cGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGa                                                                                                      >  1:585390/1‑150 (MQ=255)                                         gCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCg                                                                                                <  2:296173/151‑1 (MQ=255)                                          cAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGt                                                                                               >  2:65414/1‑151 (MQ=255)                                           aGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTg                                                                                              >  1:1635/1‑151 (MQ=255)                                             aCGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGa                                                                                             >  2:783712/1‑150 (MQ=255)                                              cGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGa                                                                                             >  2:537250/1‑149 (MQ=255)                                                   tCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTAt                                                                                      >  2:246002/1‑151 (MQ=255)                                                       gATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTcc                                                                                  >  1:199089/1‑151 (MQ=255)                                                       gATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTcc                                                                                  >  2:760297/1‑151 (MQ=255)                                                         ttACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCt                                                                                >  1:504730/1‑151 (MQ=255)                                                            cacCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTcga                                                                             <  1:621247/151‑1 (MQ=255)                                                                gTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGaa                                                                         <  1:402657/151‑1 (MQ=255)                                                                  cccATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAg                                                                        <  2:375798/150‑1 (MQ=255)                                                                  cccATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGc                                                                       >  2:147160/1‑151 (MQ=255)                                                                         cccGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATg                                                                   <  2:720280/148‑1 (MQ=255)                                                                          ccgccgGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCAt                                                               <  1:175412/151‑1 (MQ=255)                                                                          ccgccgGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCAt                                                               <  2:804844/151‑1 (MQ=255)                                                                             ccgGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCg                                                            >  2:801595/1‑151 (MQ=255)                                                                               ggAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGt                                                          <  1:674414/151‑1 (MQ=255)                                                                                  aGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCAt                                                       <  1:221818/151‑1 (MQ=255)                                                                                           aaTCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAag                                                >  2:352125/1‑149 (MQ=255)                                                                                            aTCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGc                                             >  2:789710/1‑151 (MQ=255)                                                                                            aTCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGc                                             >  1:313320/1‑151 (MQ=255)                                                                                            aTCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGc                                             <  1:386537/151‑1 (MQ=255)                                                                                             tCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAagag                                              >  2:916507/1‑149 (MQ=255)                                                                                                aaTACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAgg                                          >  2:197152/1‑150 (MQ=255)                                                                                                aaTACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTAACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGa                                         <  2:604334/151‑1 (MQ=255)                                                                                                 aTACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGAt                                        >  1:705811/1‑151 (MQ=255)                                                                                                   aCAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATc                                       <  2:658706/150‑1 (MQ=255)                                                                                                      aTCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTgg                                    >  2:805402/1‑150 (MQ=255)                                                                                                      aTCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGt                                   <  1:616433/151‑1 (MQ=255)                                                                                                          gCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGc                                 >  2:896508/1‑149 (MQ=255)                                                                                                          gCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGc                                 >  1:238410/1‑149 (MQ=255)                                                                                                           ccAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGc                              >  1:129995/1‑151 (MQ=255)                                                                                                             aaaTGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCg                            >  2:571238/1‑151 (MQ=255)                                                                                                                     gCCCGGCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTgg                     >  2:596134/1‑150 (MQ=255)                                                                                                                         ggCTGATACCAACGTCAGTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAATCGCTGGCATCCGGTCATGTAAAAGAGCAGGATCTGGTGCAGCCGCTCGTGGAAGGt                >  2:853998/1‑151 (MQ=255)                                                                                                                         ggCTGATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAgg                 >  2:448162/1‑150 (MQ=255)                                                                                                                             gATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCt              <  1:708734/149‑1 (MQ=255)                                                                                                                             gATACCAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCt              <  1:192270/149‑1 (MQ=255)                                                                                                                                 ccAACGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGa        <  1:923871/151‑1 (MQ=255)                                                                                                                                   aaCGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGata      <  1:915986/151‑1 (MQ=255)                                                                                                                                   aaCGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGat       >  1:88829/1‑150 (MQ=255)                                                                                                                                   aaCGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGat       >  2:201173/1‑150 (MQ=255)                                                                                                                                     cGTCACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGATATc    <  1:372311/151‑1 (MQ=255)                                                                                                                                        cACTAAAGTGGGGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGATATCGt  <  1:438734/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                           CAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGTATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCAAATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGTGGAAGACAGGGCCAACGCACTGCTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGTCATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGATATCGT  >  NC_000913/713929‑714213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑