Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	869,818	A→C	E136D (GAA→GAC)	gsiB →	glutathione ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	869,818	0	A	C	100.0%	211.4 / NA	59	E136D (GAA→GAC)	gsiB	glutathione ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/28);  total (31/28)

ACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGG  >  NC_000913/869673‑869961                                                                                                                                                  |                                                                                                                                                acacCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGcgat                                                                                                                                            >  2:413633/1‑151 (MQ=255) acacCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGcga                                                                                                                                             >  2:689636/1‑150 (MQ=255)  cacCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGcgatc                                                                                                                                           <  2:59204/151‑1 (MQ=255)      gTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCgatcgatc                                                                                                                                       >  2:723790/1‑151 (MQ=255)      gTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCgatcgatc                                                                                                                                       <  1:58152/151‑1 (MQ=255)         aaaTTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCg                                                                                                                                     >  1:920043/1‑150 (MQ=255)          aaTTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGAGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGa                                                                                                                                    >  2:653600/1‑150 (MQ=255)           aTTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGAc                                                                                                                                   >  2:560300/1‑150 (MQ=255)              gCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACaa                                                                                                                                 >  1:398240/1‑149 (MQ=255)               cGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACgg                                                                                                                              >  1:607323/1‑151 (MQ=255)                  gAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTa                                                                                                                            >  1:128953/1‑150 (MQ=255)                       cATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGAtt                                                                                                                      <  2:861549/151‑1 (MQ=255)                             aTTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTc                                                                                                                <  2:529870/151‑1 (MQ=255)                                 cAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAAc                                                                                                            <  2:909698/151‑1 (MQ=255)                                  aGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCTCCTTAAACGCTATAACCTGTATAAGAATATTGCGAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACa                                                                                                           <  2:440422/151‑1 (MQ=255)                                   ggATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAg                                                                                                          <  2:57726/151‑1 (MQ=255)                                    gATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGc                                                                                                         >  1:312108/1‑151 (MQ=255)                                         cACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGtt                                                                                                     >  1:528200/1‑150 (MQ=255)                                                 tCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGtt                                                                                            >  2:228645/1‑151 (MQ=255)                                                 tCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGt                                                                                             <  1:278991/150‑1 (MQ=255)                                                       ccgcGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTttatta                                                                                       >  2:778603/1‑150 (MQ=255)                                                           ggcggTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATAtt                                                                                  <  1:869552/151‑1 (MQ=255)                                                           ggcggTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATAtt                                                                                  <  2:838764/151‑1 (MQ=255)                                                              ggTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCtt                                                                               <  2:468315/151‑1 (MQ=255)                                                              ggTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCtt                                                                               <  2:366558/151‑1 (MQ=255)                                                              ggTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCtt                                                                               <  2:35631/151‑1 (MQ=255)                                                                tGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGGATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGc                                                                             >  2:507154/1‑151 (MQ=255)                                                                tGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGc                                                                             >  1:521481/1‑151 (MQ=255)                                                                    aGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCAt                                                                         <  1:413642/151‑1 (MQ=255)                                                                           cTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCg                                                                   >  1:270466/1‑150 (MQ=255)                                                                             ggACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCg                                                                   <  2:56509/148‑1 (MQ=255)                                                                             ggACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGa                                                                  <  2:819613/149‑1 (MQ=255)                                                                              gACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACcg                                                               <  2:510199/151‑1 (MQ=255)                                                                                ccGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACcgcg                                                             >  1:70091/1‑151 (MQ=255)                                                                                  gggCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCgat                                                           <  2:439155/151‑1 (MQ=255)                                                                                      cAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGAtt                                                       >  1:246341/1‑151 (MQ=255)                                                                                       aGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGAttt                                                      <  2:698835/151‑1 (MQ=255)                                                                                           aTCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCAcc                                                  >  2:259099/1‑151 (MQ=255)                                                                                             ccGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCg                                                 <  2:432994/150‑1 (MQ=255)                                                                                                 cGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAgc                                            >  2:154958/1‑151 (MQ=255)                                                                                                   aaTCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCGgcgc                                          >  1:167546/1‑151 (MQ=255)                                                                                                   aaTCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAgcgc                                          <  2:907712/151‑1 (MQ=255)                                                                                                    atcatcTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCt                                         >  2:561701/1‑151 (MQ=255)                                                                                                     tcatcTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTg                                        >  1:248765/1‑151 (MQ=255)                                                                                                          ttAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGaaa                                    >  2:573205/1‑150 (MQ=255)                                                                                                            aaaCGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAat                                 >  2:185156/1‑151 (MQ=255)                                                                                                               cGCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATg                              >  1:816634/1‑151 (MQ=255)                                                                                                                gCTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATgg                             <  2:22111/151‑1 (MQ=255)                                                                                                                 cTATAACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGc                            >  1:887652/1‑151 (MQ=255)                                                                                                                   ataACCTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGc                            <  1:684222/149‑1 (MQ=255)                                                                                                                       ccTGTATAAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAgg                        >  1:492478/1‑149 (MQ=255)                                                                                                                           tataAGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGAtt                   <  2:398231/150‑1 (MQ=255)                                                                                                                               aGAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGttt              >  2:772120/1‑151 (MQ=255)                                                                                                                                gAATATTGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGtttt             <  1:462709/151‑1 (MQ=255)                                                                                                                                      tGCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTAGcc        <  2:462204/150‑1 (MQ=255)                                                                                                                                       gCTAAAACGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCgg      >  2:868869/1‑151 (MQ=255)                                                                                                                                          aaaaCGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTg    <  2:521467/150‑1 (MQ=255)                                                                                                                                          aaaaCGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTg    <  1:793716/150‑1 (MQ=255)                                                                                                                                           aaaCGGACGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTggg  >  1:871271/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                ACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGG  >  NC_000913/869673‑869961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑