Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	987,537	A→C	intergenic (‑555/+48)	ompF ← / ← asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	987,537	0	A	C	100.0%	151.9 / NA	47	intergenic (‑555/+48)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/28);  total (19/28)

GCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCG  >  NC_000913/987389‑987684                                                                                                                                                      |                                                                                                                                                    gCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGcaa                                                                                                                                                   <  2:874849/151‑1 (MQ=255)    aTTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGcaaa                                                                                                                                                  <  1:160187/149‑1 (MQ=255)           aaGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATaaaaa                                                                                                                                          <  2:523490/150‑1 (MQ=255)               aaaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTG‑CAAATAGAATTGTTGACTTTAAAAACAGATAAATTTACAACAaaaaaaaaaaaaaa                                                                                                                                      >  1:846632/1‑148 (MQ=255)                aaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGc                                                                                                                                    <  2:356561/151‑1 (MQ=255)                aaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTG‑GAAAAAAATTTGTTGGATTTAAAAAAAAAAAAATTTACAGCAAAGAaaaaaaaaaa                                                                                                                                      >  2:134933/1‑147 (MQ=255)                  aaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTTGTTGACTTAACAAAAAAAAAACTTGACAGCAAAGATaaaaaaaaa                                                                                                                                      >  2:531106/1‑145 (MQ=255)                      aaTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGc                                                                                                                                <  1:420992/149‑1 (MQ=255)                      aaTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGc                                                                                                                                <  1:29108/149‑1 (MQ=255)                      aaTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAAAAGATTGGTTGACTTAACAAAAAAAAAAATTTACAGCAaaaaaaaaaaaaaa                                                                                                                                      >  1:908235/1‑141 (MQ=255)                      aaTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTG‑CAAATAAATTTCTTGTCTTAACAAAAAAATAAATTGACAGCAAAGATAAAAAAAAAAACAGCgg                                                                                                                              >  1:48641/1‑151 (MQ=255)                          cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTG‑GCAAAAAATTTCTTTACTTTACAAACAGAAAAAATTAAAGCAAAAAAAAAAAAAAAAACAGCggg                                                                                                                             >  1:276456/1‑148 (MQ=255)                           gCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCt                                                                                                                         <  1:234/151‑1 (MQ=255)                           gCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTG‑GAAAAAAAATGCTTTAATTTACAAAAAAATAAATTTACAACAaaaaaaaaaaaaaaacccacggg                                                                                                                             >  2:811646/1‑136 (MQ=255)                            cAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTg                                                                                                                        <  2:96366/151‑1 (MQ=255)                            cAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTG‑CAAAAAGATTTCTTTGCTTTACAAACAAAAAACTTGACAGCAAAGATAAAAAAAAAAACAGCggggg                                                                                                                           >  1:30873/1‑146 (MQ=255)                                             aGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTa                                                                                                        <  2:393019/150‑1 (MQ=255)                                                  ttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTa                                                                                                   <  1:24783/150‑1 (MQ=255)                                                      gcgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTG‑GAAAAAAAATGCTTGACTTAAAAAAAAAAAAAATTTACAGCAAAAAAAAAAAAAAAAACAACGGGGGCGGGGTCTTTGAGAAGCtaaaataaaa                                                                                                >  1:723040/1‑149 (MQ=255)                                                        gTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTG‑GACATAAAATTTTTTCATTTACAAACAGAGAAATTGACAACACAGATAAAAAAAAAGCAAAGGGGGGCTGGGGCTTCTAGTCGCTTAATTAGAAACtc                                                                                            >  1:495146/1‑150 (MQ=255)                                                          aaaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTgg                                                                                           <  1:186054/150‑1 (MQ=255)                                                           aaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCg                                                                                         <  1:101748/151‑1 (MQ=255)                                                              gTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTa                                                                                      <  2:398042/151‑1 (MQ=255)                                                                       tAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGTAAAAAAAATTGTTTG‑CTTTAAAAAAAAATAAAATGATAACAAAAATAAAAAAAAAAACAACGGGCGCCGGGGCTTTTAGAAGCGAAAATAAAAAGAGGCGGTACGCgg                                                                                 >  2:396102/1‑147 (MQ=255)                                                                         atatTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACg                                                                           <  1:664921/151‑1 (MQ=255)                                                                           atTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTg                                                                         <  1:26595/151‑1 (MQ=255)                                                                               cGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGaa                                                                     <  2:880756/151‑1 (MQ=255)                                                                                  aaCAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACgg                                                                  <  1:813138/151‑1 (MQ=255)                                                                                       gatgatCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAatca                                                             <  1:795680/151‑1 (MQ=255)                                                                                           atCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAatcacatc                                                         <  2:195724/151‑1 (MQ=255)                                                                                                 aTGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacgtac                                                   <  1:37493/151‑1 (MQ=255)                                                                                                   ggCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacgtacgt                                                 <  2:90051/151‑1 (MQ=255)                                                                                                    gCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGtt                                                <  1:148463/151‑1 (MQ=255)                                                                                                           tG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCAc                                          <  1:905970/150‑1 (MQ=255)                                                                                                               aaaTAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGcc                                       >  2:44631/1‑150 (MQ=255)                                                                                                                 aTAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGt                                    <  2:81657/151‑1 (MQ=255)                                                                                                                  tAGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGtt                                   >  1:868247/1‑151 (MQ=255)                                                                                                                   aGATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTa                                  <  2:18646/151‑1 (MQ=255)                                                                                                                    gATTGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTAc                                 <  1:606520/151‑1 (MQ=255)                                                                                                                       tGCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGt                               <  2:868797/150‑1 (MQ=255)                                                                                                                        gCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTaa                             <  2:592822/151‑1 (MQ=255)                                                                                                                        gCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTa                              >  1:556381/1‑150 (MQ=255)                                                                                                                        gCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTa                              >  1:556743/1‑150 (MQ=255)                                                                                                                        gCTTGACTTAACAAACAGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTa                              >  2:869719/1‑150 (MQ=255)                                                                                                                                     aacaGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGtt                <  1:120871/151‑1 (MQ=255)                                                                                                                                       caGATAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTc               >  1:65252/1‑150 (MQ=255)                                                                                                                                          aTAACTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCaaa            >  2:908478/1‑150 (MQ=255)                                                                                                                                            aaCTTGACAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAAcc         >  1:318056/1‑151 (MQ=255)                                                                                                                                                   cAGCAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCg  <  1:1090/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTG‑CAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTCAAAACCAAGACCG  >  NC_000913/987389‑987684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑