Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,021,095	A→C	intergenic (‑176/‑43)	sulA ← / → sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,021,095	0	A	C	100.0%	201.7 / NA	52	intergenic (‑176/‑43)	sulA/sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/26);  total (26/26)

GATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTAATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCG  >  NC_000913/1020951‑1021240                                                                                                                                                 |                                                                                                                                                  gATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCAtgtg                                                                                                                                              >  2:213928/1‑150 (MQ=255)  aTGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGat                                                                                                                                            >  1:534745/1‑150 (MQ=255)      acagtacaTCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCAt                                                                                                                                        >  2:143654/1‑151 (MQ=255)            caTCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCtttt                                                                                                                                  <  2:9607/151‑1 (MQ=255)                     acaacaAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTttatgttatg                                                                                                                          >  2:605977/1‑150 (MQ=255)                      caacaaAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGAt                                                                                                                        >  1:770226/1‑151 (MQ=255)                          aaaGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTa                                                                                                                    <  2:216383/151‑1 (MQ=255)                          aaaGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTa                                                                                                                    <  2:277168/151‑1 (MQ=255)                           aaGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTaa                                                                                                                   <  2:23685/151‑1 (MQ=255)                                   cccTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAAtt                                                                                                           <  2:306127/151‑1 (MQ=255)                                      tATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAAtttt                                                                                                         >  2:521196/1‑150 (MQ=255)                                              gAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGc                                                                                                <  2:583794/151‑1 (MQ=255)                                               aaaGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGcc                                                                                               >  2:612218/1‑151 (MQ=255)                                                  gagCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCctct                                                                                            <  1:768030/151‑1 (MQ=255)                                                        cGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCtat                                                                                       >  2:361651/1‑150 (MQ=255)                                                          cAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCtata                                                                                      >  1:616838/1‑149 (MQ=255)                                                           aaaTTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGc                                                                                   <  2:558107/151‑1 (MQ=255)                                                               tttGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGa                                                                                <  2:651182/150‑1 (MQ=255)                                                               tttGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGa                                                                                <  1:920812/150‑1 (MQ=255)                                                                ttGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGAt                                                                               <  2:367855/150‑1 (MQ=255)                                                                  gTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCt                                                                             <  1:297664/150‑1 (MQ=255)                                                                    cGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCtat                                                                           >  2:187387/1‑150 (MQ=255)                                                                        ggTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATc                                                                      >  2:294005/1‑151 (MQ=255)                                                                         gTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTGTGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATca                                                                     <  1:372119/151‑1 (MQ=255)                                                                         gTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATca                                                                     <  1:291352/151‑1 (MQ=255)                                                                           gACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATcaca                                                                   <  1:481346/151‑1 (MQ=255)                                                                           gACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATcac                                                                    <  1:436074/150‑1 (MQ=255)                                                                           gACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATcac                                                                    <  2:700149/150‑1 (MQ=255)                                                                               ggAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGa                                                                >  2:712168/1‑150 (MQ=255)                                                                                gAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAAt                                                              >  2:191923/1‑151 (MQ=255)                                                                                gAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAAt                                                              >  2:68658/1‑151 (MQ=255)                                                                                    aCATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCt                                                          <  1:132424/151‑1 (MQ=255)                                                                                       tAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGc                                                       >  2:898323/1‑151 (MQ=255)                                                                                        aaaTTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGc                                                       <  2:175967/150‑1 (MQ=255)                                                                                           ttAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAAc                                                    >  1:890336/1‑150 (MQ=255)                                                                                           ttAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACg                                                   >  1:861054/1‑151 (MQ=255)                                                                                             aaTCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGtt                                                 <  1:848929/151‑1 (MQ=255)                                                                                               tCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTgg                                               >  2:878016/1‑151 (MQ=255)                                                                                                   gCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGcac                                           >  1:210773/1‑151 (MQ=255)                                                                                                   gCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGca                                            <  2:880093/150‑1 (MQ=255)                                                                                                    ccccTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGcac                                           >  2:475701/1‑150 (MQ=255)                                                                                                          aaGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGaa                                    <  1:159094/151‑1 (MQ=255)                                                                                                            gAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAAt                                   >  2:670964/1‑150 (MQ=255)                                                                                                                aaGTTGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCg                               <  1:250572/150‑1 (MQ=255)                                                                                                                   ttGCCTATTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATc                            >  1:281630/1‑150 (MQ=255)                                                                                                                         aTTTTCGTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGt                       <  2:780168/149‑1 (MQ=255)                                                                                                                               gTAGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGt               >  1:567555/1‑151 (MQ=255)                                                                                                                                 aGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGtt              >  2:799316/1‑150 (MQ=255)                                                                                                                                 aGTTAACGGATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGtt              >  1:348761/1‑150 (MQ=255)                                                                                                                                         gATCCGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTg      >  2:695097/1‑150 (MQ=255)                                                                                                                                            ccGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCg  <  1:442445/151‑1 (MQ=255)                                                                                                                                            ccGTTCATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCg  <  2:713589/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTAATGTGAATCATTCTTTTATGTTATGATTTTAAAAGGAATTTTATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCG  >  NC_000913/1020951‑1021240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑