Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,052	A→C	D56A (GAT→GCT)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,052	0	A	C	100.0%	141.0 / NA	39	D56A (GAT→GCT)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/17);  total (22/17)

ACCCAACGTTCGATACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAG  >  NC_000913/1097905‑1098185                                                                                                                                                    |                                                                                                                                      aCCCAACGTTCGATACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGctct                                                                                                                                    >  1:506137/1‑151 (MQ=255)       cGTTCGATACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGCGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTACTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAg                                                                                                                               >  2:563797/1‑150 (MQ=255)         ttCGATACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCgg                                                                                                                            >  2:810130/1‑151 (MQ=255)             aTACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGtt                                                                                                                        >  2:313116/1‑151 (MQ=255)              tACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTc                                                                                                                       >  1:444291/1‑151 (MQ=255)                 ccAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGc                                                                                                                     >  2:705915/1‑150 (MQ=255)                  cAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCAc                                                                                                                   <  2:855998/151‑1 (MQ=255)                       gtggATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCggg                                                                                                              >  1:443662/1‑151 (MQ=255)                          gATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGcc                                                                                                           <  1:527360/151‑1 (MQ=255)                                ggCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGtt                                                                                                     <  1:802633/151‑1 (MQ=255)                                    cTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGAtt                                                                                                 >  2:277065/1‑151 (MQ=255)                                         cAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTAtt                                                                                            <  2:105592/151‑1 (MQ=255)                                             gCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGa                                                                                        <  2:889419/151‑1 (MQ=255)                                                aTTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGc                                                                                      <  2:358275/150‑1 (MQ=255)                                                  tCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCaa                                                                                    <  1:569709/150‑1 (MQ=255)                                                   ccTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGc                                                                                  >  1:34261/1‑151 (MQ=255)                                                    cTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCt                                                                                 >  1:450433/1‑151 (MQ=255)                                                       aGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTAc                                                                               >  1:17116/1‑150 (MQ=255)                                                        ggCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTAc                                                                               >  2:470594/1‑149 (MQ=255)                                                        ggCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACa                                                                              <  2:740574/150‑1 (MQ=255)                                                           aaGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGca                                                                          <  1:207275/151‑1 (MQ=255)                                                           aaGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGc                                                                           >  1:441168/1‑150 (MQ=255)                                                                    agCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGa                                                                  >  1:701492/1‑150 (MQ=255)                                                                     gCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGaaa                                                                <  1:680072/151‑1 (MQ=255)                                                                      cATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAAt                                                               >  1:281450/1‑151 (MQ=255)                                                                            aaaGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGa                                                          >  1:257523/1‑150 (MQ=255)                                                                                cGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCt                                                     <  1:821901/151‑1 (MQ=255)                                                                                 ggAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCtt                                                    >  1:585629/1‑151 (MQ=255)                                                                                    gaTAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTg                                                 <  1:436964/151‑1 (MQ=255)                                                                                    gaTAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTg                                                 <  2:822539/151‑1 (MQ=255)                                                                                      tAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGtt                                               >  1:703457/1‑151 (MQ=255)                                                                                                gctgATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACttttt                                      <  2:303990/150‑1 (MQ=255)                                                                                                   gATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCg                                    <  1:56521/149‑1 (MQ=255)                                                                                                        tGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGAGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACATGCTCACCCTGCAATGCTGCACCCTTCTGTTCCACTTTTTCGcc                                  >  2:559319/1‑146 (MQ=255)                                                                                                         gCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGa                              >  1:334512/1‑149 (MQ=255)                                                                                                         gCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGCTCTTAAAGCGGCGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGa                              >  1:287078/1‑149 (MQ=255)                                                                                                                                  aaaTGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGc    >  2:578168/1‑150 (MQ=255)                                                                                                                                  aaaTGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCa   <  1:286795/151‑1 (MQ=255)                                                                                                                                   aaTGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACAACCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAg  <  2:135467/151‑1 (MQ=255)                                                                                                                                     tGCTGGCAGGGCGCGCTCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTCCGCCTGAAGGATACCGGTATGGCCAGCCAAATGcgg  >  2:443121/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                      ACCCAACGTTCGATACCCAATGGTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAG  >  NC_000913/1097905‑1098185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑