Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,145,255 A→C N106H (AAT→CAT)  rluC → 23S rRNA pseudouridine(955,2504,2580) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,145,2550AC98.4% 210.7 / NA 62N106H (AAT→CAT) rluC23S rRNA pseudouridine(955,2504,2580) synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (30/31);  minor base G (1/0);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAG  >  NC_000913/1145107‑1145399
                                                                                                                                                    |                                                                                                                                                 
tGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGGGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCAt                                                                                                                                                 >  1:364120/1‑151 (MQ=255)
     ataAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTTGCCACATCTGCAAAAGGGGGCGGCGCTGGCGGACGTCCTCTTATATGAAGATGATCACATCCTGGTGGTGCATaaaac                                                                                                                                            >  2:699369/1‑149 (MQ=255)
       aaaCTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCtt                                                                                                                                          <  1:288067/151‑1 (MQ=255)
       aaaCTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGGGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGGGCTGCATAAACCtt                                                                                                                                          >  1:364670/1‑151 (MQ=255)
        aaCTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTc                                                                                                                                         <  1:335852/151‑1 (MQ=255)
              aaGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCCCCTCTGCAAAAGGGGGCGGCGCTGGCGGGCGTCATTTTATATGAAGATGATCACATCCTGGGGCTGCATAAACCTTCCGGGAc                                                                                                                                   >  2:712825/1‑151 (MQ=255)
                gCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTAcgg                                                                                                                                 <  1:230464/151‑1 (MQ=255)
                gCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTAcgg                                                                                                                                 <  1:814171/151‑1 (MQ=255)
                  gggTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCCAAAGGGGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCCTAAAACTTCCGGTAcggcg                                                                                                                               >  2:805310/1‑151 (MQ=255)
                  gggTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTAcggcg                                                                                                                               <  1:476410/151‑1 (MQ=255)
                    gTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGt                                                                                                                             <  1:870658/151‑1 (MQ=255)
                      gatgaGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGGCGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAAACTTCCGGTAcggcgg                                                                                                                              >  2:694514/1‑148 (MQ=255)
                        tgaGGTGCGTATTCCACCGGTTCGCGTTGCTGCGCGGGAAGAAGAGGCGGGTTCGCCACCACTGCAAAAGGGGGCGGCGCTGGGGGAAGTCATCTTCTATTAAAATGAACAAATCCTGGTGGCGGATAAACCTTCCGGTAcggcgg                                                                                                                              >  2:523699/1‑146 (MQ=255)
                         gaGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCCAAAGGGGGCGGCGCTGGCGGGAGTCATTTTATATAAAGATGATCACATCCTGGTGCTGCATAAAACTTCCGGTAcggcgg                                                                                                                              >  2:803273/1‑145 (MQ=255)
                         gaGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑At                                                                                                                        <  2:364161/150‑1 (MQ=255)
                         gaGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGGCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTAcggcgggta                                                                                                                           >  2:275936/1‑145 (MQ=255)
                                 tATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCCAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAAACTTCCGGTACGGCGGGTC‑ATggggg                                                                                                                   >  2:306150/1‑147 (MQ=255)
                                  aTTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGGCGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGGAC‑ATGGCGGCAggg                                                                                                              >  2:856569/1‑149 (MQ=255)
                                     ccACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGGAC‑ATtgcgggcgggggt                                                                                                           >  2:367193/1‑143 (MQ=255)
                                        ccGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTaa                                                                                                        <  1:82595/151‑1 (MQ=255)
                                         cGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAg                                                                                                       <  1:670755/151‑1 (MQ=255)
                                             tCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCtt                                                                                                    <  1:650893/150‑1 (MQ=255)
                                                cgTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGc                                                                                                <  1:446400/151‑1 (MQ=255)
                                                 gTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGGGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGGGCTGCATAAACCTTCCCGTACCGCGGGAC‑AAGGGGGGCGTGGGTTAAGCtttcgcg                                                                                               >  2:734264/1‑146 (MQ=255)
                                                  ttGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACAAACGCAGAAGGTGGGGGCGCTGGCGGGCGGGATCTTAGATGAAGAAGATCAGATACTGGGGTTGCATAAACCTTCCGGCACGGCGGTAG‑AAAGAGGGCGTGGGTTAAGCATCgg                                                                                                 >  1:692952/1‑148 (MQ=255)
                                                   tGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGt                                                                                              <  1:833834/150‑1 (MQ=255)
                                                    gCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTa                                                                                            <  2:366156/151‑1 (MQ=255)
                                                       gAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTg                                                                                         <  1:349403/151‑1 (MQ=255)
                                                         gCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGa                                                                                        <  2:318745/150‑1 (MQ=255)
                                                          cGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGGGCTGCATAAACCTTCCGGTACGGCGGGACAAGGGGGGCGGGGGTTTAAGTTTTGGGGtttt                                                                                           >  2:645406/1‑145 (MQ=255)
                                                                gaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGc                                                                                <  2:642133/151‑1 (MQ=255)
                                                                gaagaGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGc                                                                                <  1:798246/151‑1 (MQ=255)
                                                                   gagGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCggg                                                                             <  2:383736/151‑1 (MQ=255)
                                                                     ggCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCg                                                                           <  2:40362/151‑1 (MQ=255)
                                                                       cGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGtt                                                                         <  1:38029/151‑1 (MQ=255)
                                                                        ggTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTg                                                                        >  1:6222/1‑151 (MQ=255)
                                                                           ttCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTgcg                                                                      <  1:46756/150‑1 (MQ=255)
                                                                           ttCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGATTAAACTTTGGCGTTATTGGAGGTTTTGGGGGGtt                                                                         >  2:769939/1‑147 (MQ=255)
                                                                                  caTCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGaa                                                               <  1:119385/150‑1 (MQ=255)
                                                                                   aTCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAg                                                              >  2:722277/1‑150 (MQ=255)
                                                                                   aTCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGGGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGaa                                                               >  2:276046/1‑149 (MQ=255)
                                                                                      tGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGcgcg                                                          >  2:239973/1‑151 (MQ=255)
                                                                                       gCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGcgcg                                                          <  2:90056/150‑1 (MQ=255)
                                                                                       gCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGCAC‑ATTGAGGCCGTGGATTAAGCATCGGCGGTTTTGTAGGTTTGTGGGCGTTGCGCCCGGAAGCGCgg                                                         >  2:190368/1‑151 (MQ=255)
                                                                                        cAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCgg                                                         >  1:330591/1‑150 (MQ=255)
                                                                                         aaaaGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGGGGGTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGtt                                                       >  1:369134/1‑151 (MQ=255)
                                                                                           aaGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGGGGGTTAAGCTTCGGCGGTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTcc                                                     >  2:298950/1‑151 (MQ=255)
                                                                                                    gcgcTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGGTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTgg                                            >  2:268497/1‑151 (MQ=255)
                                                                                                      gcTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGtt                                          >  1:534636/1‑151 (MQ=255)
                                                                                                         ggCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCAt                                       >  1:846434/1‑151 (MQ=255)
                                                                                                          gCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGGGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATc                                      >  2:538387/1‑151 (MQ=255)
                                                                                                                 tCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCtt                                 >  2:497078/1‑149 (MQ=255)
                                                                                                                 tCATCTTATATGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAATGGTTTAAGCTTCGGCGTTATTGAAGGGTTGCGGGCGGTGCGCCCGGAAGCGCGGTTCCCTGAACTGGTTCATCGTCtt                                 >  2:185027/1‑149 (MQ=255)
                                                                                                                       tataTGAAGATGATCACATCCTGGTGCTGCATAAGCCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGAcc                             <  1:907767/147‑1 (MQ=255)
                                                                                                                          atGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGaca                       <  2:196656/150‑1 (MQ=255)
                                                                                                                           tGAAGATGATCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACAcc                     >  1:747855/1‑151 (MQ=255)
                                                                                                                                  gatCACATCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACACCTCAGgt               <  2:425474/150‑1 (MQ=255)
                                                                                                                                      acaTCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACACCTCAGGTGtttt          <  1:741747/151‑1 (MQ=255)
                                                                                                                                      acaTCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACACCTCAGGTGtttt          <  1:417357/151‑1 (MQ=255)
                                                                                                                                       caTCCTGGTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTg         <  2:484495/151‑1 (MQ=255)
                                                                                                                                             ggTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACACTTCAGGTGTTTTGCTGGTa   <  1:367201/151‑1 (MQ=255)
                                                                                                                                              gTGCTGCATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAg  <  2:364662/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGGCGCTGGCGGACGTCATCTTATATGAAGATGATCACATCCTGGTGCTGAATAAACCTTCCGGTACGGCGGTAC‑ATGGCGGCAGTGGTTTAAGCTTCGGCGTTATTGAAGGTTTGCGGGCGTTGCGCCCGGAAGCGCGGTTCCTTGAACTGGTTCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAG  >  NC_000913/1145107‑1145399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑