Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,930	A→C	N307H (AAC→CAC)	prfA →	peptide chain release factor RF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,930	0	A	C	100.0%	171.9 / NA	47	N307H (AAC→CAC)	prfA	peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/22);  total (25/22)

CAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTAACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGC  >  NC_000913/1265783‑1266075                                                                                                                                                    |                                                                                                                                                  cAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGaccgtcacc                                                                                                                                                >  1:677711/1‑151 (MQ=255) cAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGaccgtcacc                                                                                                                                                <  2:717833/151‑1 (MQ=255) cAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGaccgtcacc                                                                                                                                                >  2:563186/1‑151 (MQ=255)     aCGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTAc                                                                                                                                            >  2:465619/1‑151 (MQ=255)         aCGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTAc                                                                                                                                        <  1:11076/151‑1 (MQ=255)         aCGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTAc                                                                                                                                        <  1:44312/151‑1 (MQ=255)              cacaACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCGACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACtt                                                                                                                                   <  2:195116/151‑1 (MQ=255)                   caTAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGc                                                                                                                              >  2:13951/1‑151 (MQ=255)                     tAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGc                                                                                                                              >  1:703902/1‑149 (MQ=255)                     tAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGc                                                                                                                              >  1:368268/1‑149 (MQ=255)                      aaaaaCAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAgg                                                                                                                           >  2:648389/1‑151 (MQ=255)                         aaCAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGgc                                                                                                                        >  1:618855/1‑151 (MQ=255)                              aGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGtt                                                                                                                   >  1:27529/1‑151 (MQ=255)                                 tAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCGGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTAcc                                                                                                                >  1:848764/1‑151 (MQ=255)                                  aaaGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCg                                                                                                               <  1:115102/151‑1 (MQ=255)                                  aaaGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCg                                                                                                               <  2:852466/151‑1 (MQ=255)                                     gCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATc                                                                                                            >  1:55843/1‑151 (MQ=255)                                            cTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCAt                                                                                                     >  1:700814/1‑151 (MQ=255)                                             tGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCAt                                                                                                     >  2:90487/1‑150 (MQ=255)                                                 ctcGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAAcc                                                                                                >  1:686874/1‑151 (MQ=255)                                                   cGGTGCTCGCATCCGCGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAAcc                                                                                                <  1:594417/149‑1 (MQ=255)                                                    ggTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTg                                                                                              >  1:143402/1‑150 (MQ=255)                                                           gCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTa                                                                                      <  2:45511/151‑1 (MQ=255)                                                             aTCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTAcc                                                                                    >  1:99499/1‑151 (MQ=255)                                                             aTCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTAcc                                                                                    >  2:104377/1‑151 (MQ=255)                                                                    ctgctgAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTgg                                                                              <  2:60625/150‑1 (MQ=255)                                                                      gctgAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGa                                                                             <  1:153727/149‑1 (MQ=255)                                                                         gAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGaa                                                                         <  2:676327/150‑1 (MQ=255)                                                                         gAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGa                                                                          >  2:548248/1‑149 (MQ=255)                                                                         gAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGa                                                                          <  2:489684/149‑1 (MQ=255)                                                                           aaTGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTg                                                                      >  2:698941/1‑151 (MQ=255)                                                                            aTGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGa                                                                     <  1:618289/151‑1 (MQ=255)                                                                                 aaaaCGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGa                                                                 >  1:63405/1‑150 (MQ=255)                                                                                      gCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTaa                                                           <  1:650863/151‑1 (MQ=255)                                                                                            aGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGa                                                     <  2:450877/151‑1 (MQ=255)                                                                                                cGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATg                                                 >  1:235486/1‑151 (MQ=255)                                                                                                    gCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTg                                              <  2:884077/150‑1 (MQ=255)                                                                                                    gCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGa                                             <  2:72008/151‑1 (MQ=255)                                                                                                      gTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGAtt                                           <  1:57183/151‑1 (MQ=255)                                                                                                          aCCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACTGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAAc                                       <  2:890031/151‑1 (MQ=255)                                                                                                          aCCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGaa                                        <  1:402903/150‑1 (MQ=255)                                                                                                           cccGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAAcc                                      <  1:517103/151‑1 (MQ=255)                                                                                                            ccGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCg                                     >  2:437917/1‑151 (MQ=255)                                                                                                                         ctgGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAAc                        >  1:71220/1‑151 (MQ=255)                                                                                                                          tgGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAAc                        >  2:804400/1‑150 (MQ=255)                                                                                                                           ggggAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACAt                      >  1:716837/1‑151 (MQ=255)                                                                                                                            gggAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACAt                      >  2:233505/1‑150 (MQ=255)                                                                                                                                gTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGc                 <  2:460868/151‑1 (MQ=255)                                                                                                                                   gCGATCGCAGCGCCCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCCCCGCATCACCCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGa              <  2:613221/151‑1 (MQ=255)                                                                                                                                              gACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTggcgg   >  2:63044/1‑151 (MQ=255)                                                                                                                                               accgtcaccgtaCTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTggcggc  >  2:574697/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CAGGACGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTAACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGC  >  NC_000913/1265783‑1266075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑