Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,296,022	A→C	R700R (CGT→CGG)	adhE ←	fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,296,022	0	A	C	100.0%	225.3 / NA	61	R700R (CGT→CGG)	adhE	fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/36);  total (25/36)

AACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTC  >  NC_000913/1295878‑1296165                                                                                                                                                 |                                                                                                                                                aaCGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCt                                                                                                                                           >  1:112546/1‑151 (MQ=255)      tACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCg                                                                                                                                       <  2:154867/150‑1 (MQ=255)        cAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGAt                                                                                                                                    <  1:229899/151‑1 (MQ=255)          aaTCAGCAGGGCGTTTGCCAGACCGTGTGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGAttt                                                                                                                                  <  1:353578/151‑1 (MQ=255)          aaTCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGAttt                                                                                                                                  <  1:341398/151‑1 (MQ=255)           aTCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGAtttt                                                                                                                                 <  1:369457/151‑1 (MQ=255)                    gCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCtt                                                                                                                        >  2:132621/1‑151 (MQ=255)                     cGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTc                                                                                                                       >  2:213025/1‑151 (MQ=255)                     cGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTc                                                                                                                       >  2:104448/1‑151 (MQ=255)                     cGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTc                                                                                                                       <  1:709178/151‑1 (MQ=255)                      gTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCg                                                                                                                      >  1:784460/1‑151 (MQ=255)                        ttGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTg                                                                                                                    >  2:839181/1‑151 (MQ=255)                         tGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTg                                                                                                                    <  1:531871/150‑1 (MQ=255)                          gCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGt                                                                                                                  <  1:380269/151‑1 (MQ=255)                                ccGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGATCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGAc                                                                                                             <  2:710703/150‑1 (MQ=255)                                  gTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCt                                                                                                          >  1:365927/1‑151 (MQ=255)                                      ggAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCa                                                                                                      <  1:329441/151‑1 (MQ=255)                                           atGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGatat                                                                                                 >  2:454756/1‑151 (MQ=255)                                             ggAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTc                                                                                               <  1:867481/151‑1 (MQ=255)                                                  tGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTc                                                                                           >  2:546089/1‑150 (MQ=255)                                                    ggAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTcagc                                                                                        >  2:578490/1‑151 (MQ=255)                                                          cAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTc                                                                                  >  2:757642/1‑151 (MQ=255)                                                              ttGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTg                                                                              >  1:339210/1‑151 (MQ=255)                                                                  gcgcCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCt                                                                           <  2:460015/150‑1 (MQ=255)                                                                  gcgcCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCt                                                                           <  1:627774/150‑1 (MQ=255)                                                                     ccATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCag                                                                       <  1:248966/151‑1 (MQ=255)                                                                       aTTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCagag                                                                     <  1:95648/151‑1 (MQ=255)                                                                         tGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGc                                                                    <  2:462163/150‑1 (MQ=255)                                                                             tGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAG‑TTCAGTGCCTGCAGAGCCTGAcc                                                              >  1:687916/1‑151 (MQ=255)                                                                                  tACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATc                                                           >  1:128183/1‑150 (MQ=255)                                                                                   acacCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCa                                                          <  1:490170/150‑1 (MQ=255)                                                                                    cacCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATc                                                           >  1:258328/1‑148 (MQ=255)                                                                                    cacCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCaga                                                        <  2:112664/151‑1 (MQ=255)                                                                                      cccAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCagag                                                       <  2:665011/150‑1 (MQ=255)                                                                                            aaGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAg                                                <  2:284257/151‑1 (MQ=255)                                                                                             aGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGa                                               <  1:306647/151‑1 (MQ=255)                                                                                              ggCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGAt                                              <  2:218759/151‑1 (MQ=255)                                                                                               gCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATg                                             <  1:189697/151‑1 (MQ=255)                                                                                               gCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATg                                             <  2:478888/151‑1 (MQ=255)                                                                                                cGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGc                                            <  1:467263/151‑1 (MQ=255)                                                                                                cGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGc                                            >  2:692668/1‑151 (MQ=255)                                                                                                 gTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGcc                                           >  1:184320/1‑151 (MQ=255)                                                                                                       aaaCGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACa                                     <  2:126048/151‑1 (MQ=255)                                                                                                          cgcgATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGaa                                  <  2:435404/151‑1 (MQ=255)                                                                                                          cgcgATACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGa                                   >  1:699595/1‑150 (MQ=255)                                                                                                           gcgATTCCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGaa                                  <  1:635022/150‑1 (MQ=255)                                                                                                              aTACCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACAt                              <  2:526147/151‑1 (MQ=255)                                                                                                                aCCCGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATa                             >  2:610004/1‑150 (MQ=255)                                                                                                                 cccGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAg                           <  2:557129/151‑1 (MQ=255)                                                                                                                  ccGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAg                           >  1:631768/1‑150 (MQ=255)                                                                                                                  ccGCGATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGc                          >  2:434000/1‑151 (MQ=255)                                                                                                                   cgcgATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCt                         <  2:236196/151‑1 (MQ=255)                                                                                                                     cgATAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTc                       <  2:339202/151‑1 (MQ=255)                                                                                                                       aTAGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCa                     <  2:665996/151‑1 (MQ=255)                                                                                                                         aGTCGCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATg                   <  1:747437/151‑1 (MQ=255)                                                                                                                             gCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGt               >  2:7723/1‑151 (MQ=255)                                                                                                                             gCTGCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGt               >  2:83857/1‑151 (MQ=255)                                                                                                                                gCACTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAg            <  2:373480/151‑1 (MQ=255)                                                                                                                                  aCTGTGAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGtt          >  1:615131/1‑151 (MQ=255)                                                                                                                                     gtgAACACGTTCCCGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACt       >  2:203684/1‑151 (MQ=255)                                                                                                                                          cacGTTCCCGCGCTACCGGAGTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTGCAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTc  <  1:557416/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                AACGTTACAAATCAGCAGGGCGTTTGCCAGACCGTGCGGAATATGGAACTGGGAACCCAGTTTGTGCGCCATTGAGTGACATACACCCAGGAAGGCGTTCGCAAACGCGATACCCGCGATAGTCGCTGCACTGTGAACACGTTCACGCGCTACCGGATTTTTAGACCCTTCGTGGTAGGACGCTGGCAGATATTCTTTCAGCAGTTTCAGTGCCTGCAGAGCCTGACCATCAGAGAACTCAGATGCCAGTACAGAAACATAAGCTTCCATGGCGTGAGTTACTGCGTC  >  NC_000913/1295878‑1296165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑