Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,317,315	A→C	D367E (GAT→GAG)	trpB ←	tryptophan synthase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,317,315	0	A	C	100.0%	134.2 / NA	36	D367E (GAT→GAG)	trpB	tryptophan synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/18);  total (18/18)

AATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCC  >  NC_000913/1317167‑1317458                                                                                                                                                     |                                                                                                                                                aaTGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTtctc                                                                                                                                               >  2:34035/1‑151 (MQ=255)     cgcCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCggg                                                                                                                                           <  1:338977/151‑1 (MQ=255)        cttcttTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGttt                                                                                                                                        >  1:290050/1‑151 (MQ=255)           cttTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTcg                                                                                                                                     >  1:723360/1‑151 (MQ=255)               gcgcTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCgcgc                                                                                                                                  <  1:29408/150‑1 (MQ=255)                   tCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGcatcat                                                                                                                             >  2:203664/1‑151 (MQ=255)                     cTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCAttt                                                                                                                           >  1:387576/1‑151 (MQ=255)                        cAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTc                                                                                                                         <  2:160361/150‑1 (MQ=255)                        cAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCa                                                                                                                        <  2:900962/151‑1 (MQ=255)                          aCTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAAc                                                                                                                      >  1:781901/1‑151 (MQ=255)                            tGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGc                                                                                                                    >  2:281603/1‑151 (MQ=255)                             gggCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACg                                                                                                                     <  1:592550/149‑1 (MQ=255)                                 aaaCAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATgg                                                                                                                >  1:135455/1‑150 (MQ=255)                                    cAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCa                                                                                                            <  1:912545/151‑1 (MQ=255)                                    cAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCa                                                                                                            <  1:547658/151‑1 (MQ=255)                                      gagaTTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAgg                                                                                                          <  2:69028/151‑1 (MQ=255)                                        gaTTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGc                                                                                                        <  2:431033/151‑1 (MQ=255)                                             gTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTggg                                                                                                   <  1:518363/151‑1 (MQ=255)                                                gCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGggagg                                                                                                >  1:382868/1‑151 (MQ=255)                                                  gTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGAt                                                                                              <  1:899241/151‑1 (MQ=255)                                                          aGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgcgc                                                                                      <  2:174192/151‑1 (MQ=255)                                                          aGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgcgc                                                                                      <  2:899006/151‑1 (MQ=255)                                                             tttCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCgg                                                                                   >  2:474882/1‑151 (MQ=255)                                                                           ttCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGt                                                                     >  1:676184/1‑151 (MQ=255)                                                                                        gAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCg                                                         <  2:353576/150‑1 (MQ=255)                                                                                         aaCGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGtt                                                       >  2:443443/1‑151 (MQ=255)                                                                                                aaGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAgg                                                <  1:318116/151‑1 (MQ=255)                                                                                                              gccgcGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtc                                   >  1:39962/1‑150 (MQ=255)                                                                                                                cgcgACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTtcatc                                >  1:347982/1‑151 (MQ=255)                                                                                                                    aCCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTcatcat                              <  2:654295/149‑1 (MQ=255)                                                                                                                       ggAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTa                         <  2:829933/151‑1 (MQ=255)                                                                                                                        gAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTaa                        <  2:527101/151‑1 (MQ=255)                                                                                                                           aGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAAt                       >  2:879974/1‑149 (MQ=255)                                                                                                                               tAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGacac                 >  1:148297/1‑151 (MQ=255)                                                                                                                                       aGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATc           >  2:110184/1‑149 (MQ=255)                                                                                                                                               ctctTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTcc  >  2:777854/1‑150 (MQ=255)                                                                                                                                                tctTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTcc  >  2:437977/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                AATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGTGCAGGCACAGCGTTTTGAAGGCTTCAAGGGCTTCATCATCGGTAATAGACACGTAATCAGCGCGTCC  >  NC_000913/1317167‑1317458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑