| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,324,533 | A→C | M146L (ATG→CTG) | yciO → | putative RNA binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,324,533 | 0 | A | C | 98.2% | 193.7 / ‑3.8 | 57 | M146L (ATG→CTG) | yciO | putative RNA binding protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (31/25); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01 | |||||||||||
TATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCG > NC_000913/1324386‑1324673 | tataCCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACtgc > 2:640448/1‑151 (MQ=255) aCCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACtgctgc < 2:164915/151‑1 (MQ=255) aCCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACtgctgc > 1:381984/1‑151 (MQ=255) ccTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGcc > 2:165708/1‑151 (MQ=255) cATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGc > 1:353379/1‑151 (MQ=255) ccTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAg < 1:592986/150‑1 (MQ=255) ccTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGc < 1:224052/151‑1 (MQ=255) ccTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGc < 2:715014/151‑1 (MQ=255) cTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCg > 2:32578/1‑151 (MQ=255) gAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGa < 2:792390/150‑1 (MQ=255) gACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTAcc > 1:563073/1‑151 (MQ=255) cGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCg > 1:465061/1‑150 (MQ=255) cGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGa < 2:400357/151‑1 (MQ=255) gaaggaagTGCCACGCCGTCTGTTGCAGG‑AAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAAt > 1:852019/1‑151 (MQ=255) aaggaagTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAAt > 2:471900/1‑151 (MQ=255) aggaagTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATc > 2:240554/1‑151 (MQ=255) gTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGAcc < 2:106474/151‑1 (MQ=255) ccACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCgg > 1:423487/1‑151 (MQ=255) cACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGga < 1:536167/151‑1 (MQ=255) aCGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGga > 1:593426/1‑150 (MQ=255) aCGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGga > 2:171609/1‑150 (MQ=255) cGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTTCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGga > 1:456061/1‑149 (MQ=255) gCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGga > 2:295003/1‑148 (MQ=255) ccGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGgaaga > 2:194741/1‑150 (MQ=255) tCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAAt < 1:705481/150‑1 (MQ=255) tGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTa > 2:731546/1‑150 (MQ=255) tGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAg < 2:139677/150‑1 (MQ=255) aGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTaa > 1:531179/1‑145 (MQ=255) aGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCg < 1:607886/151‑1 (MQ=255) aaaCGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTgg > 1:345915/1‑151 (MQ=255) aaaaCCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTa < 1:835590/133‑1 (MQ=255) aaaaCCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTa > 2:835572/1‑133 (MQ=255) aaaaCCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAAc < 1:395125/151‑1 (MQ=255) aaaaCCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAAc > 1:633478/1‑151 (MQ=255) aTCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGt > 1:602664/1‑149 (MQ=255) aTCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACGTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAg < 2:484996/151‑1 (MQ=255) cGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGt > 1:811838/1‑147 (MQ=255) aTGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTg > 1:701348/1‑150 (MQ=255) tGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGat > 1:898377/1‑151 (MQ=255) cGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGatta < 2:851648/151‑1 (MQ=255) gtgtGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGattat < 2:645156/151‑1 (MQ=255) ttCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAcggc > 2:841630/1‑151 (MQ=255) tCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAcggc < 1:194744/150‑1 (MQ=255) tCTAACCCTATCGCCCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAcggcg > 1:416915/1‑151 (MQ=255) aaCCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAcggcg < 2:84790/148‑1 (MQ=255) aCCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAcggcg < 2:486322/147‑1 (MQ=255) gCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACGTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGc < 2:840541/151‑1 (MQ=255) aGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGa > 1:277133/1‑150 (MQ=255) cGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAAcc < 2:711661/151‑1 (MQ=255) gTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCg < 1:907116/151‑1 (MQ=255) tACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCgcc > 2:778276/1‑149 (MQ=255) aGCATTGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGtt < 2:457631/151‑1 (MQ=255) tGGGCGAACCGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTg > 1:513383/1‑150 (MQ=255) ccGATGCTTTCCACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCg < 1:306000/151‑1 (MQ=255) ccACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTACTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGcc < 2:381976/151‑1 (MQ=255) cACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCg > 2:691468/1‑151 (MQ=255) cACTTCGCTACTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCg > 2:374121/1‑151 (MQ=255) | TATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCG > NC_000913/1324386‑1324673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |