Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,453,097	T→G	P182P (CCA→CCC)	paaZ ←	oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,453,097	0	T	G	100.0%	187.3 / NA	53	P182P (CCA→CCC)	paaZ	oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/33);  total (20/33)

GTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATCCACCTTCTTTC  >  NC_000913/1452951‑1453244                                                                                                                                                   |                                                                                                                                                    gTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGtt                                                                                                                                                 >  1:887648/1‑151 (MQ=255)   gAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTtg                                                                                                                                               <  2:409429/151‑1 (MQ=255)         caccacATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGatgatg                                                                                                                                         >  1:910299/1‑151 (MQ=255)             acaTCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGAtggctg                                                                                                                                     >  2:916443/1‑151 (MQ=255)                    ggCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAGCAAGACCACTATCGACAATAGATTTCACCATCGCCTGAGGCAGTTGTGCCGTCGCGGTGGCGGTTTTGATGATGGCTCGCAtt                                                                                                                                <  2:152810/149‑1 (MQ=255)                           cAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGc                                                                                                                       <  1:916244/151‑1 (MQ=255)                              atgatCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCAc                                                                                                                    <  2:697591/151‑1 (MQ=255)                                      acaaGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGc                                                                                                            >  2:488155/1‑151 (MQ=255)                                            cGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCtt                                                                                                      >  1:549492/1‑151 (MQ=255)                                              ccAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCtttt                                                                                                    <  2:154280/151‑1 (MQ=255)                                                 gCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTcc                                                                                                  >  2:358447/1‑150 (MQ=255)                                                   aCTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGc                                                                                               <  1:443661/151‑1 (MQ=255)                                                   aCTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGc                                                                                               <  2:290576/151‑1 (MQ=255)                                                     tACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGGTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCAt                                                                                             >  1:334692/1‑151 (MQ=255)                                                      aCCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCAt                                                                                             <  1:149316/150‑1 (MQ=255)                                                              tCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCcagc                                                                                     >  1:526933/1‑150 (MQ=255)                                                                aGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAgg                                                                                  <  1:721465/151‑1 (MQ=255)                                                                aGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAgg                                                                                  >  1:537548/1‑151 (MQ=255)                                                                     aaTTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGaa                                                                              <  2:592665/150‑1 (MQ=255)                                                                     aaTTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGa                                                                               <  2:835237/149‑1 (MQ=255)                                                                         gcgcCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTaa                                                                         <  1:824316/151‑1 (MQ=255)                                                                         gcgcCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTa                                                                          <  1:71473/150‑1 (MQ=255)                                                                          cgcCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTaaa                                                                        <  1:694462/151‑1 (MQ=255)                                                                              ttCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCg                                                                    >  2:30611/1‑151 (MQ=255)                                                                               tCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCg                                                                    <  2:371470/150‑1 (MQ=255)                                                                                cGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGtt                                                                  <  1:625746/151‑1 (MQ=255)                                                                                cGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGtt                                                                  <  2:614345/151‑1 (MQ=255)                                                                                        aGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGc                                                           >  2:640502/1‑150 (MQ=255)                                                                                            cACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCTCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGc                                                      >  1:666523/1‑151 (MQ=255)                                                                                                aTCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACg                                                  >  2:220258/1‑151 (MQ=255)                                                                                                  cGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGcc                                                >  2:272417/1‑151 (MQ=255)                                                                                                  cGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGcc                                                <  1:376807/151‑1 (MQ=255)                                                                                                    aCAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTg                                              <  2:588936/151‑1 (MQ=255)                                                                                                      aaTTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGAc                                            >  2:888857/1‑151 (MQ=255)                                                                                                       aTTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACt                                           >  1:306149/1‑151 (MQ=255)                                                                                                          gATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACttt                                         <  1:86615/150‑1 (MQ=255)                                                                                                             ttCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAg                                      <  2:420877/150‑1 (MQ=255)                                                                                                                          gAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGgcg                        >  1:581531/1‑151 (MQ=255)                                                                                                                          gAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGgc                         <  2:223610/150‑1 (MQ=255)                                                                                                                          gAGTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGgc                         <  2:887628/150‑1 (MQ=255)                                                                                                                            gTCAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGgcgc                       <  2:455394/150‑1 (MQ=255)                                                                                                                              cAGTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGcgcgcg                    <  2:521683/151‑1 (MQ=255)                                                                                                                                gTTGGGCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGc                  <  1:287300/151‑1 (MQ=255)                                                                                                                                   gggCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCaa                <  2:644163/150‑1 (MQ=255)                                                                                                                                    ggCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAAt              <  2:685474/151‑1 (MQ=255)                                                                                                                                    ggCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAAt              <  2:736215/151‑1 (MQ=255)                                                                                                                                     gCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATc             <  2:686994/151‑1 (MQ=255)                                                                                                                                     gCCGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATc             <  1:120867/151‑1 (MQ=255)                                                                                                                                      ccGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATcc            <  2:157442/151‑1 (MQ=255)                                                                                                                                       cGTCGCGGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATcc            >  2:511689/1‑150 (MQ=255)                                                                                                                                          cgcgGTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATCCACct        >  1:483453/1‑151 (MQ=255)                                                                                                                                              gTAGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATCCACcttctt    <  2:585956/151‑1 (MQ=255)                                                                                                                                                aGCGGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATCCACCTTCTTTc  >  1:825175/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGACTTTGAGGTCAGTAAATGGCGCGCGGCAAATCCACCTTCTTTC  >  NC_000913/1452951‑1453244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑