Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,492,314	T→G	intergenic (‑185/‑156)	mokB ← / → trg	regulatory peptide/methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,492,314	0	T	G	100.0%	208.0 / NA	54	intergenic (‑185/‑156)	mokB/trg	regulatory peptide/methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/31);  total (23/31)

TCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTTTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGTAAACCTGGACGAGAGA  >  NC_000913/1492168‑1492461                                                                                                                                                   |                                                                                                                                                    tCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTa                                                                                                                                                   <  2:743722/149‑1 (MQ=255)   gTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGcc                                                                                                                                                >  1:496809/1‑150 (MQ=255)       ttCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTc                                                                                                                                            <  1:139920/150‑1 (MQ=255)        tCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCg                                                                                                                                           <  1:851554/150‑1 (MQ=255)           gATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGtt                                                                                                                                         <  1:672937/149‑1 (MQ=255)               cccGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTActctc                                                                                                                                   <  1:720831/151‑1 (MQ=255)                 cGGTTAGCCTGAAAACAGAAAGTCTCCGGCACCCGCAGGCATCCTATGAGGTTTTCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCtt                                                                                                                                 <  2:61350/151‑1 (MQ=255)                 cGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCtt                                                                                                                                 <  1:669561/151‑1 (MQ=255)                    ttAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCa                                                                                                                               >  1:693249/1‑150 (MQ=255)                            aaaaCAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAg                                                                                                                      <  1:774368/151‑1 (MQ=255)                               aCAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCAt                                                                                                                   <  1:481982/151‑1 (MQ=255)                                    aaGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAAtt                                                                                                              <  2:653728/151‑1 (MQ=255)                                     aGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAAttt                                                                                                             <  1:56477/151‑1 (MQ=255)                                      gTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAAtttt                                                                                                            <  1:923233/151‑1 (MQ=255)                                             gCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGc                                                                                                      >  2:652481/1‑150 (MQ=255)                                             gCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCa                                                                                                     <  1:416331/151‑1 (MQ=255)                                              cACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCaa                                                                                                    <  1:805588/151‑1 (MQ=255)                                               aCCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCaat                                                                                                   >  2:800861/1‑151 (MQ=255)                                                cccGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCaata                                                                                                  <  2:375353/151‑1 (MQ=255)                                                 ccGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCaata                                                                                                  >  1:325566/1‑150 (MQ=255)                                                     aGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGACATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGCTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAAttg                                                                                              >  2:755606/1‑150 (MQ=255)                                                           ccTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTaa                                                                                        <  2:121874/150‑1 (MQ=255)                                                             tATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTATaaaa                                                                                      >  2:62319/1‑150 (MQ=255)                                                                gAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAAtgt                                                                                  <  2:290407/151‑1 (MQ=255)                                                                 aGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAAtgtg                                                                                 <  1:289095/151‑1 (MQ=255)                                                                 aGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAAtgtg                                                                                 <  2:628892/151‑1 (MQ=255)                                                                   gTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGAc                                                                               >  1:783877/1‑151 (MQ=255)                                                                           aGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAag                                                                         >  2:44192/1‑149 (MQ=255)                                                                           aGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAag                                                                         >  1:37831/1‑149 (MQ=255)                                                                            gggACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAgg                                                                      <  1:305794/151‑1 (MQ=255)                                                                                cGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGtttt                                                                  <  2:751045/151‑1 (MQ=255)                                                                                  aaaaTAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTg                                                                <  2:177827/151‑1 (MQ=255)                                                                                  aaaaTAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTg                                                                <  2:819217/151‑1 (MQ=255)                                                                                        atCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCAt                                                           >  2:638538/1‑150 (MQ=255)                                                                                            cTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGt                                                       >  2:888456/1‑150 (MQ=255)                                                                                                      ttGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTc                                             >  2:910032/1‑150 (MQ=255)                                                                                                         cGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGt                                         <  2:92934/151‑1 (MQ=255)                                                                                                         cGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGt                                         >  2:628446/1‑151 (MQ=255)                                                                                                                   tCCAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACt                               >  1:276989/1‑151 (MQ=255)                                                                                                                    ccAGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACtt                              >  2:618145/1‑151 (MQ=255)                                                                                                                      aGAATTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTc                            <  2:117839/151‑1 (MQ=255)                                                                                                                        aaTTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCt                           >  2:764618/1‑150 (MQ=255)                                                                                                                        aaTTTTTCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCt                           >  1:156874/1‑150 (MQ=255)                                                                                                                           ttttCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATc                        >  1:452870/1‑150 (MQ=255)                                                                                                                            tttCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATc                        >  2:716747/1‑149 (MQ=255)                                                                                                                             ttCGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATCCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAg                      <  2:802924/150‑1 (MQ=255)                                                                                                                               cGTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAg                   <  1:652496/151‑1 (MQ=255)                                                                                                                                gTCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGt                  <  2:159318/151‑1 (MQ=255)                                                                                                                                 tCATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGt                  >  1:211900/1‑150 (MQ=255)                                                                                                                                  cATTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGTa                 <  1:572907/150‑1 (MQ=255)                                                                                                                                   aTTCGGGTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGTaaa               <  2:309678/151‑1 (MQ=255)                                                                                                                                        ggTTAGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGTAAACCTgg          >  2:227375/1‑151 (MQ=255)                                                                                                                                            aGCCAGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGTAAACCTGGACg       >  1:352748/1‑150 (MQ=255)                                                                                                                                                aGTGTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGTAAACCTGGACgagaga  <  1:891293/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCATTCGGGTTAGCCAGTTTAGCCATTCGTTACTCTCTTCATTCCAATAGCATTAATTTTCTATGCAATAATTGTTGTAAAAATGTGACGCAAAGAGGTTTTTGGTCATAAGTAATTACCGTCAAGTGCCGATGACTTTCTATCAGGAGTAAACCTGGACGAGAGA  >  NC_000913/1492168‑1492461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑