Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,523,163	A→C	intergenic (‑98/‑144)	yncD ← / → yncE	putative iron outer membrane transporter/ATP‑binding protein, periplasmic, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,523,163	0	A	C	100.0%	211.2 / NA	54	intergenic (‑98/‑144)	yncD/yncE	putative iron outer membrane transporter/ATP‑binding protein, periplasmic, function unknown
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/25);  total (29/25)

AAGCAGTGCGGGCAAAACGGTCTGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAAAATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTTCATCAAAGGGAGTCGTCA  >  NC_000913/1523021‑1523307                                                                                                                                               |                                                                                                                                                 aaGCAGTGCGGGCAAAACGGTCTGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGt                                                                                                                                          >  2:472824/1‑151 (MQ=255)  aGCAGTGCGGGCAAAACGGTCTGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTc                                                                                                                                         <  1:495926/151‑1 (MQ=255)     aGTGCGGGCAAAACGGTCTGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTa                                                                                                                                      <  2:718287/151‑1 (MQ=255)         cGGGCAAAACGGTCTGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACgg                                                                                                                                  <  1:602888/151‑1 (MQ=255)           ggCAAAACGGTCTGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCa                                                                                                                                <  2:359861/151‑1 (MQ=255)                 aCGGTCTGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCCCAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAAt                                                                                                                          >  2:90736/1‑151 (MQ=255)                       tGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTcc                                                                                                                     >  2:189228/1‑150 (MQ=255)                        gTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCca                                                                                                                    <  2:103000/150‑1 (MQ=255)                          cggacggaAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCcaca                                                                                                                  <  2:232706/150‑1 (MQ=255)                          cggacggaAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACAt                                                                                                                 >  2:908836/1‑151 (MQ=255)                              cggaAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTg                                                                                                              >  2:241457/1‑150 (MQ=255)                                  aaaaaTCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGaaa                                                                                                         <  2:746513/151‑1 (MQ=255)                                          tCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATg                                                                                                   <  2:10209/149‑1 (MQ=255)                                          tCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATg                                                                                                   <  1:539866/149‑1 (MQ=255)                                           cATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGAt                                                                                                 <  2:119579/150‑1 (MQ=255)                                                aaaaaGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCAt                                                                                           >  1:920210/1‑151 (MQ=255)                                                   aaGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTcc                                                                                        >  2:198922/1‑151 (MQ=255)                                                         ggTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCAttt                                                                                   >  1:597093/1‑150 (MQ=255)                                                             aaGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATaa                                                                               >  2:241123/1‑150 (MQ=255)                                                             aaGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAAc                                                                              <  2:601281/151‑1 (MQ=255)                                                                   gggAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGc                                                                        <  2:249986/151‑1 (MQ=255)                                                                    ggAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCg                                                                       >  2:146838/1‑151 (MQ=255)                                                                     gAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGt                                                                      <  1:919089/151‑1 (MQ=255)                                                                           gCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACgat                                                                >  2:183978/1‑151 (MQ=255)                                                                               tCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGAtt                                                            >  1:424589/1‑151 (MQ=255)                                                                               tCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGAtt                                                            >  2:300252/1‑151 (MQ=255)                                                                                cATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGAtt                                                            >  2:343735/1‑150 (MQ=255)                                                                                 aTGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTAc                                                          <  1:820882/151‑1 (MQ=255)                                                                                 aTGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTAc                                                          <  2:380273/151‑1 (MQ=255)                                                                                   ggTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACg                                                         <  2:543758/150‑1 (MQ=255)                                                                                   ggTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGc                                                        >  1:648592/1‑151 (MQ=255)                                                                                     tAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCtt                                                      >  2:121015/1‑151 (MQ=255)                                                                                               gTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCAt                                            >  2:462510/1‑151 (MQ=255)                                                                                                 aaaTTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATtg                                          >  2:818439/1‑151 (MQ=255)                                                                                                      tATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGa                                       >  2:918058/1‑149 (MQ=255)                                                                                                      tATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGa                                       >  2:246022/1‑149 (MQ=255)                                                                                                        tGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAagc                                    >  2:179532/1‑150 (MQ=255)                                                                                                          gAAAATGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAagcagc                                 >  2:462746/1‑151 (MQ=255)                                                                                                            aaaTGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCaa                               <  1:908189/151‑1 (MQ=255)                                                                                                            aaaTGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCaa                               <  1:630574/151‑1 (MQ=255)                                                                                                               tGAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCaaaa                             >  2:219086/1‑150 (MQ=255)                                                                                                                gAAACGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAat                           <  2:679244/151‑1 (MQ=255)                                                                                                                   aCGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATc                        <  1:146584/151‑1 (MQ=255)                                                                                                                   aCGGCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATc                        <  1:609638/151‑1 (MQ=255)                                                                                                                      gCACAATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGt                     <  2:408752/151‑1 (MQ=255)                                                                                                                       cacaATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGtt                    >  1:404916/1‑151 (MQ=255)                                                                                                                       cacaATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGtt                    <  2:45672/151‑1 (MQ=255)                                                                                                                         caATACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTtc                   >  2:61885/1‑150 (MQ=255)                                                                                                                          aaTACGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTtcat                 >  1:237884/1‑151 (MQ=255)                                                                                                                             aCGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTtcatca               >  1:428812/1‑150 (MQ=255)                                                                                                                              cGTTAAGTAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTTCATCaaa             <  2:883526/151‑1 (MQ=255)                                                                                                                                     tAATTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTTCATCAAAGGGAgtc      >  1:282345/1‑151 (MQ=255)                                                                                                                                      aaTTGAGAACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTTCATCAAAGGGAgtcg     >  2:755886/1‑151 (MQ=255)                                                                                                                                          gagaACATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTTCATCAAAGGGAGTCGTCa  <  2:610048/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                 AAGCAGTGCGGGCAAAACGGTCTGTCGGACGGAAAAAATCTTCATGAAAAAAGCCAGGTTAAGAATGGGAAAACGCCGTCATGGTAATGAAATTGTAAATTTATGGAAAATGAAACGGCACAATACGTTAAGTAATTGAGAAAATTGTAGTCGTAACGGCAAGAAATGCTCCACATTTGAGAAAATAATGATTACCATTCCCATTTATAACAAGAGCGTAACGATGATTACGCTTAGCGAAGCATTGTGAAGCAGCAAAAATATCGGTTCATCAAAGGGAGTCGTCA  >  NC_000913/1523021‑1523307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑