Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,607,228	A→C	K77T (AAA→ACA)	lsrG →	autoinducer‑2 (AI‑2) degrading protein LsrG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,607,228	0	A	C	100.0%	218.0 / NA	57	K77T (AAA→ACA)	lsrG	autoinducer‑2 (AI‑2) degrading protein LsrG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/29);  total (28/29)

CTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATA  >  NC_000913/1607084‑1607371                                                                                                                                                 |                                                                                                                                                ctGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTgg                                                                                                                                            >  1:873813/1‑150 (MQ=255)   gTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTgg                                                                                                                                            <  2:358401/148‑1 (MQ=255)   gTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTgg                                                                                                                                            <  2:49924/148‑1 (MQ=255)      cAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCtt                                                                                                                                      >  2:721984/1‑151 (MQ=255)           agaagGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATg                                                                                                                                 <  1:746893/151‑1 (MQ=255)             aagGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGAc                                                                                                                               >  2:53085/1‑151 (MQ=255)                 cAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCggg                                                                                                                           >  1:301082/1‑151 (MQ=255)                 cAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCggg                                                                                                                           >  1:494275/1‑151 (MQ=255)                  aaTTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCggg                                                                                                                           <  2:195049/150‑1 (MQ=255)                   aTTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGcc                                                                                                                         <  2:284124/151‑1 (MQ=255)                       gcgcTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGt                                                                                                                     <  1:195197/151‑1 (MQ=255)                           ttCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTaaa                                                                                                                  >  1:232668/1‑150 (MQ=255)                            tCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTaaaa                                                                                                                 <  1:494980/150‑1 (MQ=255)                                        aGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCaa                                                                                                    <  2:636126/151‑1 (MQ=255)                                          gACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATg                                                                                                  <  1:629999/151‑1 (MQ=255)                                          gACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATg                                                                                                  <  1:492529/151‑1 (MQ=255)                                            cccGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGt                                                                                                <  2:129425/151‑1 (MQ=255)                                              cGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGttt                                                                                              <  1:723662/151‑1 (MQ=255)                                                 aaGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGAt                                                                                           >  1:852663/1‑151 (MQ=255)                                                  aGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATg                                                                                          >  1:304029/1‑151 (MQ=255)                                                        ttCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTg                                                                                     >  1:911981/1‑150 (MQ=255)                                                         tCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGa                                                                                    <  2:301696/150‑1 (MQ=255)                                                          cgcgCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAgg                                                                                  >  2:71054/1‑151 (MQ=255)                                                            cgcTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCg                                                                                <  1:241479/151‑1 (MQ=255)                                                               ttttATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGa                                                                               <  1:253352/149‑1 (MQ=255)                                                                  tataTCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAAttt                                                                           >  1:266458/1‑150 (MQ=255)                                                                  tataTCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAAttt                                                                           <  1:884697/150‑1 (MQ=255)                                                                      tCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCa                                                                       >  2:380794/1‑150 (MQ=255)                                                                       cTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAAt                                                                     >  1:103359/1‑151 (MQ=255)                                                                         aCGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAAttt                                                                   <  1:161406/151‑1 (MQ=255)                                                                           gAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAAtttt                                                                  >  2:310166/1‑150 (MQ=255)                                                                             aGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTAt                                                                >  1:813349/1‑150 (MQ=255)                                                                              gCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCt                                                              <  1:685909/151‑1 (MQ=255)                                                                                cTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTAc                                                            >  2:763792/1‑151 (MQ=255)                                                                                  ataAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACa                                                           >  1:429868/1‑150 (MQ=255)                                                                                    aaaGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAAtt                                                        >  2:874831/1‑151 (MQ=255)                                                                                    aaaGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAAtt                                                        >  1:21257/1‑151 (MQ=255)                                                                                      aGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTgg                                                      >  2:72715/1‑151 (MQ=255)                                                                                          gAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGt                                                   <  1:105008/150‑1 (MQ=255)                                                                                          gAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTa                                                  <  2:909473/151‑1 (MQ=255)                                                                                            aGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAAc                                                <  2:499079/151‑1 (MQ=255)                                                                                                  aGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTg                                          >  1:804856/1‑151 (MQ=255)                                                                                                   gTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGa                                         <  1:492803/151‑1 (MQ=255)                                                                                                      gCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACgg                                      <  1:215574/151‑1 (MQ=255)                                                                                                              tAAAACCACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTaaa                               >  2:387717/1‑150 (MQ=255)                                                                                                                    cACGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGt                        >  2:529127/1‑151 (MQ=255)                                                                                                                     aCGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTc                       >  1:230184/1‑151 (MQ=255)                                                                                                                      cGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCt                      >  2:271416/1‑151 (MQ=255)                                                                                                                      cGCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCt                      >  1:878113/1‑151 (MQ=255)                                                                                                                       gCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTg                     <  1:554648/151‑1 (MQ=255)                                                                                                                       gCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTg                     >  2:381433/1‑151 (MQ=255)                                                                                                                       gCCCCACTACAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTg                     <  2:348163/151‑1 (MQ=255)                                                                                                                               aCAAAACCTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGaa              <  2:890009/150‑1 (MQ=255)                                                                                                                                     ccTGTGTCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCctct       >  1:98445/1‑151 (MQ=255)                                                                                                                                        gtgtCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCttt     <  2:194985/150‑1 (MQ=255)                                                                                                                                          gtCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTtata  <  2:248756/151‑1 (MQ=255)                                                                                                                                          gtCGCGACACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTtat   <  1:387725/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                CTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGCGTTCCATAAAACCACGCCCCACTACAAAACCTGTGTCGCGAAACTGGAATCTTTAATGACCGGGCCGCGTAAAAAACGTCTGTTCAATGGTTTGATGCCGTGAGGCGAATTTATCAATTTTATCTACAATTGGGGTAACGCGCTGACGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATA  >  NC_000913/1607084‑1607371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑