Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,657,342	A→C	F39C (TTT→TGT)	ynfC ←	UPF0257 family lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,657,342	0	A	C	100.0%	200.9 / NA	53	F39C (TTT→TGT)	ynfC	UPF0257 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/26);  total (27/26)

CCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCAAAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGTAGAGATGA  >  NC_000913/1657196‑1657482                                                                                                                                                   |                                                                                                                                             ccACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATc                                                                                                                                          >  1:745908/1‑151 (MQ=255)   aCGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAACCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCg                                                                                                                                         >  2:899590/1‑150 (MQ=255)     ggTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAAt                                                                                                                                      >  2:173286/1‑151 (MQ=255)      gTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAAtt                                                                                                                                     >  2:589202/1‑151 (MQ=255)          tattTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCAt                                                                                                                                  <  1:301670/150‑1 (MQ=255)           attTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTa                                                                                                                                <  2:416657/151‑1 (MQ=255)              ttCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTag                                                                                                                               >  2:830333/1‑149 (MQ=255)                  aGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAAc                                                                                                                         >  1:151061/1‑151 (MQ=255)                     tCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTgg                                                                                                                      >  1:320345/1‑151 (MQ=255)                      ccAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGc                                                                                                                     <  1:206718/151‑1 (MQ=255)                      ccAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGc                                                                                                                     <  1:171438/151‑1 (MQ=255)                        aGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGcc                                                                                                                    >  2:708388/1‑150 (MQ=255)                              tCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCa                                                                                                              <  1:208057/150‑1 (MQ=255)                                                   tcttcCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACct                                                                                        >  1:140858/1‑151 (MQ=255)                                                      tcCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACctc                                                                                       <  1:169937/149‑1 (MQ=255)                                                       ccGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCtgt                                                                                    <  1:375851/151‑1 (MQ=255)                                                        cGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCtgtg                                                                                   <  1:127402/151‑1 (MQ=255)                                                         gACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGc                                                                                  <  2:476395/151‑1 (MQ=255)                                                          aCAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCg                                                                                 <  2:562888/151‑1 (MQ=255)                                                          aCAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCg                                                                                 <  1:893923/151‑1 (MQ=255)                                                            aaaGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGt                                                                               >  2:806922/1‑151 (MQ=255)                                                                 cccAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACAt                                                                          >  2:398357/1‑151 (MQ=255)                                                                          acGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAgg                                                                 <  2:621051/151‑1 (MQ=255)                                                                           cGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAgg                                                                 >  1:397384/1‑150 (MQ=255)                                                                            gTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGaa                                                               <  2:475720/151‑1 (MQ=255)                                                                                      ttCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGt                                                     >  1:343176/1‑151 (MQ=255)                                                                                      ttCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGt                                                     >  2:528398/1‑151 (MQ=255)                                                                                      ttCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGt                                                     >  1:260655/1‑151 (MQ=255)                                                                                       tCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGt                                                     <  1:111690/150‑1 (MQ=255)                                                                                       tCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGt                                                     <  2:902011/150‑1 (MQ=255)                                                                                              gCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATgg                                             <  2:136447/151‑1 (MQ=255)                                                                                                tCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGgt                                            >  1:288036/1‑150 (MQ=255)                                                                                                tCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGgt                                            >  2:809293/1‑150 (MQ=255)                                                                                                   tCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGgtagt                                         >  2:190680/1‑150 (MQ=255)                                                                                                    ccATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGgtagta                                        <  1:867423/150‑1 (MQ=255)                                                                                                     cATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAAt                                      >  2:321978/1‑151 (MQ=255)                                                                                                       ttAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAAttt                                    >  2:117646/1‑151 (MQ=255)                                                                                                        tAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAAttt                                    <  2:359632/150‑1 (MQ=255)                                                                                                        tAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAAttt                                    <  1:7092/150‑1 (MQ=255)                                                                                                            gTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTAtt                               <  2:899272/151‑1 (MQ=255)                                                                                                                gAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTcac                           >  1:364420/1‑151 (MQ=255)                                                                                                                gAGTGAAATCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGCCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTc                             <  1:472646/149‑1 (MQ=255)                                                                                                                      aaTCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGt                     <  2:140627/151‑1 (MQ=255)                                                                                                                       aTCTTTTACCGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTc                    >  2:737211/1‑151 (MQ=255)                                                                                                                               ccGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCag               >  2:776309/1‑148 (MQ=255)                                                                                                                               ccGGACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCag               >  2:80161/1‑148 (MQ=255)                                                                                                                                  gACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGt          >  2:346689/1‑150 (MQ=255)                                                                                                                                  gACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGTa         <  2:705620/151‑1 (MQ=255)                                                                                                                                  gACCACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGTa         <  2:695541/151‑1 (MQ=255)                                                                                                                                     cACGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGTag        >  2:413463/1‑149 (MQ=255)                                                                                                                                       cGCAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGTAGAgat    <  2:688353/151‑1 (MQ=255)                                                                                                                                         cAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGTAGAgatga  <  2:243730/151‑1 (MQ=255)                                                                                                                                         cAGCGGATCACAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGTAGAgatg   >  2:233167/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                             CCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGTGAAATCTTTTACCGGACCACGCAGCGGATCAAAATCGAATTCATTAGAGAAACTGGCCATCTCAGGGGTAAATGAAAGTGTTACCTCTGTGCGGTCACATCCTGTGAGGAATATCGCGAGTAAGCATGGTAGTAATTTGTATTTCACAACAGTCACCAGAGAGTAGAGATGA  >  NC_000913/1657196‑1657482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑