Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,502	T→G	intergenic (‑361/‑196)	ydhZ ← / → pykF	uncharacterized protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,502	0	T	G	100.0%	214.1 / NA	59	intergenic (‑361/‑196)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/27);  total (32/27)

TTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGC  >  NC_000913/1755356‑1755640                                                                                                                                                   |                                                                                                                                           tttCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGt                                                                                                                                          >  2:609793/1‑149 (MQ=255)   tCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGa                                                                                                                                       >  1:269426/1‑150 (MQ=255)      cTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTc                                                                                                                                   >  1:570757/1‑151 (MQ=255)        ggAACGTTAAATCTTTGATACCAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCa                                                                                                                                  <  2:33780/150‑1 (MQ=255)         gAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAAc                                                                                                                                <  1:672715/151‑1 (MQ=255)          aaCGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACg                                                                                                                               >  2:184330/1‑151 (MQ=255)           aCGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGa                                                                                                                              <  2:722132/151‑1 (MQ=255)             gTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACt                                                                                                                            <  1:306506/151‑1 (MQ=255)                      ttGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCaaaa                                                                                                                       <  1:93368/147‑1 (MQ=255)                       tGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAg                                                                                                                   <  1:465682/150‑1 (MQ=255)                          tAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCAc                                                                                                               >  1:891889/1‑151 (MQ=255)                              aaTTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCAc                                                                                                           <  1:640244/151‑1 (MQ=255)                               aTTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACg                                                                                                          >  1:42929/1‑151 (MQ=255)                                 ttATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGtt                                                                                                        >  2:779164/1‑151 (MQ=255)                                   aTTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTgg                                                                                                      >  1:268693/1‑151 (MQ=255)                                     tGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGc                                                                                                    >  2:223652/1‑151 (MQ=255)                                      gTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCt                                                                                                   >  1:151278/1‑151 (MQ=255)                                          aaCAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTg                                                                                                  >  2:773308/1‑148 (MQ=255)                                          aaCAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTg                                                                                                  <  2:240411/148‑1 (MQ=255)                                          aaCAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTg                                                                                                  >  2:818915/1‑148 (MQ=255)                                           aCAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGac                                                                                              >  2:159265/1‑151 (MQ=255)                                            cAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGac                                                                                              >  1:770961/1‑150 (MQ=255)                                            cAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAA‑TTCCGCGTATAATGCGCGCCAATTGGATCTTGAATGGGTTCAGCACTTTGGACGGGAGGAATCAACGGATCAAAAACAGGCACTTCCCTTTGGCTg                                                                                                  >  1:466421/1‑145 (MQ=255)                                                  gTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGca                                                                                       <  1:326400/151‑1 (MQ=255)                                                             gAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCctct                                                                            >  2:245551/1‑151 (MQ=255)                                                             gAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCctct                                                                            >  1:114691/1‑151 (MQ=255)                                                               aaCGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGc                                                                          >  1:267267/1‑151 (MQ=255)                                                               aaCGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGc                                                                          >  2:509299/1‑151 (MQ=255)                                                                aCGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGc                                                                          >  1:617631/1‑150 (MQ=255)                                                                aCGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGc                                                                          >  2:565131/1‑150 (MQ=255)                                                                 cGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCAc                                                                        <  1:509644/151‑1 (MQ=255)                                                                        tttgttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCtttt                                                                  <  1:631581/150‑1 (MQ=255)                                                                        tttgttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCtttt                                                                  <  2:387181/150‑1 (MQ=255)                                                                          tgttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTc                                                                <  2:570742/150‑1 (MQ=255)                                                                           gttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCg                                                               >  1:288927/1‑150 (MQ=255)                                                                           gttttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCg                                                               >  2:849775/1‑150 (MQ=255)                                                                             tttCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGAt                                                             <  2:891869/150‑1 (MQ=255)                                                                                 cTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCAc                                                        >  2:553629/1‑151 (MQ=255)                                                                                 cTTTTGGATTAATGTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGt                                                           <  1:687429/148‑1 (MQ=255)                                                                                      ggATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTAt                                                    <  2:829133/150‑1 (MQ=255)                                                                                      ggATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATc                                                   >  2:365333/1‑151 (MQ=255)                                                                                           aaTTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCtt                                                >  1:537094/1‑149 (MQ=255)                                                                                              ttCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTagag                                            <  2:8764/150‑1 (MQ=255)                                                                                                cAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGa                                         <  2:638671/151‑1 (MQ=255)                                                                                                           gcgcgcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGcaccaccac                              >  2:622724/1‑151 (MQ=255)                                                                                                               gcCAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTc                          <  1:416926/151‑1 (MQ=255)                                                                                                                ccAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCg                         <  1:719429/151‑1 (MQ=255)                                                                                                                 cAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGt                        >  1:105070/1‑151 (MQ=255)                                                                                                                 cAATTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGt                        >  1:900343/1‑151 (MQ=255)                                                                                                                  aaTTGACTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGt                        >  2:518818/1‑150 (MQ=255)                                                                                                                       aCTCTTGAATGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATAcc                  <  1:831681/151‑1 (MQ=255)                                                                                                                              aaTGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGAtt             <  2:611369/149‑1 (MQ=255)                                                                                                                               aTGGTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGc          <  2:151277/151‑1 (MQ=255)                                                                                                                                 ggTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCtt        <  1:54196/151‑1 (MQ=255)                                                                                                                                 ggTTTCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCt         <  2:900323/150‑1 (MQ=255)                                                                                                                                     tCAGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCg    >  2:872108/1‑151 (MQ=255)                                                                                                                                       aGCACTTTGGACGGTAGAACTCAACGGCTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCg    <  2:890188/149‑1 (MQ=255)                                                                                                                                       aGCACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGc  <  2:193803/151‑1 (MQ=255)                                                                                                                                         cACTTTGGACGGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGc  >  1:480194/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                           TTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGC  >  NC_000913/1755356‑1755640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑