Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,842,229	T→G	intergenic (‑94/‑142)	ynjH ← / → gdhA	DUF1496 family protein/glutamate dehydrogenase, NADP‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,842,229	0	T	G	100.0%	199.8 / NA	51	intergenic (‑94/‑142)	ynjH/gdhA	DUF1496 family protein/glutamate dehydrogenase, NADP‑specific
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/28);  total (23/28)

GCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGG  >  NC_000913/1842083‑1842374                                                                                                                                                   |                                                                                                                                                  gCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGActc                                                                                                                                               >  1:653425/1‑151 (MQ=255)    aGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCg                                                                                                                                            >  1:689281/1‑151 (MQ=255)     gTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGt                                                                                                                                           >  1:503346/1‑151 (MQ=255)       gCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGtta                                                                                                                                         <  1:487772/151‑1 (MQ=255)       gCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGtt                                                                                                                                          >  1:586677/1‑150 (MQ=255)             aaTGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAAtt                                                                                                                                   >  1:105508/1‑151 (MQ=255)                ggAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGc                                                                                                                                <  1:72200/151‑1 (MQ=255)                  aaaTGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCtt                                                                                                                              >  1:298317/1‑151 (MQ=255)                   aaTGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCttt                                                                                                                             >  1:1539/1‑151 (MQ=255)                      gCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCt                                                                                                                          <  1:577707/151‑1 (MQ=255)                               acGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCAttt                                                                                                                 >  2:215782/1‑151 (MQ=255)                                 ggCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCAttttt                                                                                                               <  1:212026/151‑1 (MQ=255)                                     aaCAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCt                                                                                                           >  1:356916/1‑151 (MQ=255)                                           gCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTa                                                                                                     <  1:775692/151‑1 (MQ=255)                                             tCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTAcc                                                                                                   >  2:570933/1‑151 (MQ=255)                                              cGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCg                                                                                                  <  1:767425/151‑1 (MQ=255)                                               gACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGt                                                                                                 >  1:338478/1‑151 (MQ=255)                                                 cTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTc                                                                                                >  2:422608/1‑150 (MQ=255)                                                  tCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTcac                                                                                              >  1:310314/1‑151 (MQ=255)                                                         gAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTg                                                                                       <  2:579675/151‑1 (MQ=255)                                                            cTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATg                                                                                    >  2:894955/1‑151 (MQ=255)                                                                ttAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGtat                                                                                >  2:453104/1‑151 (MQ=255)                                                                  aaCGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGtata                                                                               <  2:498547/150‑1 (MQ=255)                                                                    cGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTc                                                                            <  1:366901/151‑1 (MQ=255)                                                                    cGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTc                                                                            <  2:270965/151‑1 (MQ=255)                                                                    cGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTc                                                                            >  2:781378/1‑151 (MQ=255)                                                                             ctctGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGc                                                                   >  1:609309/1‑151 (MQ=255)                                                                               ctGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTg                                                                    <  2:601403/148‑1 (MQ=255)                                                                                    aCTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGca                                                            <  2:342613/151‑1 (MQ=255)                                                                                        aTAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATg                                                        <  2:485467/151‑1 (MQ=255)                                                                                            cGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACAt                                                    <  2:195587/151‑1 (MQ=255)                                                                                              gTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATa                                                   >  2:711752/1‑150 (MQ=255)                                                                                                 gCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAaaca                                               <  1:395854/151‑1 (MQ=255)                                                                                                     ggTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACAt                                           <  1:500134/151‑1 (MQ=255)                                                                                                     ggTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACAt                                           <  2:601644/151‑1 (MQ=255)                                                                                                           aaaaCTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGcac                                     <  2:417173/151‑1 (MQ=255)                                                                                                            aaaCTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGcaca                                    <  1:94815/151‑1 (MQ=255)                                                                                                               cTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATc                                 >  2:56061/1‑151 (MQ=255)                                                                                                                   aGCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTAt                             <  2:653410/151‑1 (MQ=255)                                                                                                                    gCGTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTAtt                            <  1:122272/151‑1 (MQ=255)                                                                                                                      gTCTGAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTaa                          <  1:80616/151‑1 (MQ=255)                                                                                                                          gAGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAata                        <  2:105507/149‑1 (MQ=255)                                                                                                                           aGTTATCGCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAatatat                     <  1:126771/151‑1 (MQ=255)                                                                                                                                  gCATTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGtt              <  1:38444/151‑1 (MQ=255)                                                                                                                                    aTTTGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGtttt            <  1:195126/151‑1 (MQ=255)                                                                                                                                      ttGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTta           <  2:689264/150‑1 (MQ=255)                                                                                                                                       tGGTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTtat          >  2:343249/1‑150 (MQ=255)                                                                                                                                        ggTTATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTatat        >  2:496625/1‑151 (MQ=255)                                                                                                                                           tATGAGATGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCt      >  2:512249/1‑150 (MQ=255)                                                                                                                                              gagaTGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATgg  >  2:300164/1‑151 (MQ=255)                                                                                                                                              gagaTGACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATg   <  2:376184/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGG  >  NC_000913/1842083‑1842374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑