Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,856,690	T→G	E80A (GAG→GCG)	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,690	0	T	G	100.0%	214.4 / NA	55	E80A (GAG→GCG)	ydjH	putative kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/25);  total (30/25)

CAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCT  >  NC_000913/1856544‑1856826                                                                                                                                                   |                                                                                                                                         cAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCttt                                                                                                                                      <  2:37830/151‑1 (MQ=255)    aaTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCg                                                                                                                                   <  2:230800/151‑1 (MQ=255)     aTCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCgg                                                                                                                                  >  1:302239/1‑151 (MQ=255)          cATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATg                                                                                                                             >  1:727565/1‑151 (MQ=255)           aTCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATgg                                                                                                                            >  1:780712/1‑151 (MQ=255)              gTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCg                                                                                                                         >  1:836154/1‑151 (MQ=255)              gTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCg                                                                                                                         <  1:47024/151‑1 (MQ=255)                 aaTATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCgaga                                                                                                                      <  2:894339/151‑1 (MQ=255)                  atatTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCgaga                                                                                                                      >  1:886675/1‑150 (MQ=255)                    atTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAAt                                                                                                                    >  2:447013/1‑150 (MQ=255)                    atTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAAt                                                                                                                    <  1:709935/150‑1 (MQ=255)                       cAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAAt                                                                                                                >  2:520614/1‑151 (MQ=255)                        aaTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAAtt                                                                                                               >  1:644597/1‑151 (MQ=255)                        aaTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAAtt                                                                                                               >  2:214145/1‑151 (MQ=255)                            tCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGccc                                                                                                           >  2:766374/1‑151 (MQ=255)                             ccACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCt                                                                                                          >  1:557767/1‑151 (MQ=255)                                   aCTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCa                                                                                                    <  1:835495/151‑1 (MQ=255)                                      gCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCt                                                                                                 >  2:34161/1‑151 (MQ=255)                                              ggTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAAt                                                                                         >  1:125317/1‑151 (MQ=255)                                                 ttGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATAc                                                                                       <  2:134602/150‑1 (MQ=255)                                                   gtgACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGa                                                                                     <  1:717041/150‑1 (MQ=255)                                                        aaaCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAtt                                                                               >  2:708072/1‑151 (MQ=255)                                                         aaCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTa                                                                              <  2:397569/151‑1 (MQ=255)                                                           cGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAAt                                                                            >  1:334865/1‑151 (MQ=255)                                                            gTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATg                                                                           >  1:228453/1‑151 (MQ=255)                                                             tCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGc                                                                          >  1:830726/1‑151 (MQ=255)                                                                gCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCtgt                                                                       <  1:628545/151‑1 (MQ=255)                                                                    gCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCg                                                                    >  1:761792/1‑150 (MQ=255)                                                                           tccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGccc                                                             >  2:424813/1‑150 (MQ=255)                                                                            ccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGccc                                                             <  1:305855/149‑1 (MQ=255)                                                                                  ccAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACg                                                        >  2:209167/1‑148 (MQ=255)                                                                                   cAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACgaga                                                     >  2:838890/1‑150 (MQ=255)                                                                                   cAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGaa                                                    <  2:329869/151‑1 (MQ=255)                                                                                        cAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAaataat                                               <  2:491872/151‑1 (MQ=255)                                                                                         aaCGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAAtt                                              <  2:731428/151‑1 (MQ=255)                                                                                          aCGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAAttg                                             >  1:101121/1‑151 (MQ=255)                                                                                            gTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAAttgtt                                           <  1:277988/151‑1 (MQ=255)                                                                                               aaTAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGc                                         <  1:311850/150‑1 (MQ=255)                                                                                                  agagGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTc                                      >  1:260907/1‑150 (MQ=255)                                                                                                  agagGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTc                                      >  2:904386/1‑150 (MQ=255)                                                                                                     ggTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCAttt                                  <  2:267119/151‑1 (MQ=255)                                                                                                     ggTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCAttt                                  <  2:48867/151‑1 (MQ=255)                                                                                                       tATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTAt                                >  1:12346/1‑151 (MQ=255)                                                                                                        aTCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTAt                                <  1:520628/150‑1 (MQ=255)                                                                                                         tCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTAtt                               >  1:612948/1‑150 (MQ=255)                                                                                                           tATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGc                             >  2:78467/1‑150 (MQ=255)                                                                                                            aTGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGc                             <  1:88449/149‑1 (MQ=255)                                                                                                            aTGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCAt                           >  2:641917/1‑151 (MQ=255)                                                                                                              gCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCa                         <  1:225525/151‑1 (MQ=255)                                                                                                                             cAGGCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCAt           <  1:370867/150‑1 (MQ=255)                                                                                                                                gCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCg       >  2:465836/1‑151 (MQ=255)                                                                                                                                gCTTTGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCg       <  2:761774/151‑1 (MQ=255)                                                                                                                                  tttGAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGTCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGAt     >  2:791961/1‑151 (MQ=255)                                                                                                                                     gAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCt  <  2:334857/151‑1 (MQ=255)                                                                                                                                     gAATATCAATATTCGCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCt  <  1:447023/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                         CAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCT  >  NC_000913/1856544‑1856826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑