Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,696	T→G	I104M (ATT→ATG)	yeaI →	putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,696	0	T	G	100.0%	203.8 / NA	52	I104M (ATT→ATG)	yeaI	putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/23);  total (29/23)

TCAAAGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAG  >  NC_000913/1870549‑1870843                                                                                                                                                    |                                                                                                                                                    tCAAAGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCAtgttt                                                                                                                                                  >  1:642329/1‑151 (MQ=255) tCAAAGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCAtgttt                                                                                                                                                  >  2:788710/1‑151 (MQ=255) tCAAAGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCAtgttt                                                                                                                                                  >  1:224765/1‑151 (MQ=255)   aaaGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATgtttct                                                                                                                                                >  1:714507/1‑151 (MQ=255)    aaGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATgtttctt                                                                                                                                               >  1:876628/1‑151 (MQ=255)    aaGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATgtttctt                                                                                                                                               >  2:165058/1‑151 (MQ=255)            ttATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGt                                                                                                                                        <  2:900683/150‑1 (MQ=255)             tATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGtt                                                                                                                                       <  2:882577/150‑1 (MQ=255)               ttttCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAAt                                                                                                                                    >  2:412355/1‑151 (MQ=255)                 ttCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATg                                                                                                                                   <  2:708533/150‑1 (MQ=255)                 ttCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATg                                                                                                                                   >  1:108727/1‑150 (MQ=255)                  tCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGa                                                                                                                                  >  1:200044/1‑150 (MQ=255)                        tATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATAtt                                                                                                                            <  1:279579/150‑1 (MQ=255)                            gCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCAc                                                                                                                        <  2:594378/150‑1 (MQ=255)                               gACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTaac                                                                                                                    >  1:884523/1‑151 (MQ=255)                               gACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTa                                                                                                                      <  2:442313/149‑1 (MQ=255)                                aCGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTaac                                                                                                                    >  2:614944/1‑150 (MQ=255)                                      attttattttGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCATCATGTTTCTTTTCATGTTAATGAAATATTTCACTAAcaaacaaa                                                                                                             <  2:8445/151‑1 (MQ=255)                                         ttattttGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAAttt                                                                                                          >  2:303827/1‑151 (MQ=255)                                           attttGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAAtttt                                                                                                         <  2:186171/150‑1 (MQ=255)                                             tttGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTAc                                                                                                       <  1:393405/150‑1 (MQ=255)                                                gCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAac                                                                                                   >  2:803798/1‑151 (MQ=255)                                                    tactacCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTc                                                                                               >  1:2667/1‑151 (MQ=255)                                                       tacCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCAtt                                                                                            >  1:320038/1‑151 (MQ=255)                                                        acCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCAttt                                                                                           <  1:901937/151‑1 (MQ=255)                                                         ccTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCAtttt                                                                                          >  2:847122/1‑151 (MQ=255)                                                          cTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCAtttt                                                                                          >  1:670944/1‑150 (MQ=255)                                                            tatataACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTa                                                                                         >  2:912284/1‑149 (MQ=255)                                                            tatataACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTa                                                                                         >  2:348495/1‑149 (MQ=255)                                                             atataACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGc                                                                                      >  2:624917/1‑151 (MQ=255)                                                                taACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCtt                                                                                   >  2:163522/1‑151 (MQ=255)                                                                   cGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCa                                                                                <  1:797540/151‑1 (MQ=255)                                                                     aaaaCACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCAtt                                                                              <  2:768118/151‑1 (MQ=255)                                                                       aaCACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCAtttt                                                                            >  1:232243/1‑151 (MQ=255)                                                                        acacAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCAttttt                                                                           <  1:36761/151‑1 (MQ=255)                                                                                 tAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCt                                                                  >  2:354479/1‑151 (MQ=255)                                                                                  aaaaCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCtt                                                                 <  1:165058/151‑1 (MQ=255)                                                                                       tCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCt                                                             >  1:274144/1‑150 (MQ=255)                                                                                        cATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTa                                                            <  2:916471/150‑1 (MQ=255)                                                                                         aTCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTACTGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTAtt                                                          >  1:266896/1‑151 (MQ=255)                                                                                                  aTGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGaa                                                  >  2:701466/1‑150 (MQ=255)                                                                                                        tACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCAtt                                            <  1:617700/150‑1 (MQ=255)                                                                                                          ccTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGt                                          >  2:203240/1‑150 (MQ=255)                                                                                                             gATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAAtt                                      >  1:158791/1‑151 (MQ=255)                                                                                                                ggTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTAt                                    <  2:391540/150‑1 (MQ=255)                                                                                                                          gCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCaa                         <  2:836625/151‑1 (MQ=255)                                                                                                                          gCTTTGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCa                          >  1:216196/1‑150 (MQ=255)                                                                                                                            tttGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAAtt                       >  2:858934/1‑151 (MQ=255)                                                                                                                             ttGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAAtt                       >  2:26237/1‑150 (MQ=255)                                                                                                                              tGTTTTTCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTa                      <  2:25591/150‑1 (MQ=255)                                                                                                                                   ttCCTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGc                 <  2:383996/150‑1 (MQ=255)                                                                                                                                      cTTAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGt              >  1:518666/1‑150 (MQ=255)                                                                                                                                       ttAACGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTac            >  1:788695/1‑151 (MQ=255)                                                                                                                                           cGCCGTCATGTTTCTTTTCATGTTAATGAAATATTTAACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTg        <  1:223207/151‑1 (MQ=255)                                                                                                                                            gCCGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTg        >  2:218382/1‑150 (MQ=255)                                                                                                                                              cGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATc     <  2:624828/151‑1 (MQ=255)                                                                                                                                              cGTCATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATc     >  2:920107/1‑151 (MQ=255)                                                                                                                                                 cATGTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAg  <  2:884503/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TCAAAGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAG  >  NC_000913/1870549‑1870843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑