Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,641	T→G	L72R (CTT→CGT)	yoaD →	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,641	0	T	G	100.0%	180.2 / NA	50	L72R (CTT→CGT)	yoaD	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/26);  total (24/26)

GCACTCGTTACGCTCGCTTCGACCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCC  >  NC_000913/1898493‑1898783                                                                                                                                                     |                                                                                                                                               gcACTCGTTACGCTCGCTTCGACCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTc                                                                                                                                              <  2:72776/151‑1 (MQ=255)   aCTCGTTACGCTCGCTTCGACCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCg                                                                                                                                            >  1:403721/1‑151 (MQ=255)      cGTTACGCTCGCTTCGACCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCt                                                                                                                                          >  2:760212/1‑150 (MQ=255)      cGTTACGCTCGCTTCGACCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTg                                                                                                                                         <  2:566793/151‑1 (MQ=255)                gctTCGACCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGc                                                                                                                               <  2:119812/151‑1 (MQ=255)                      aCCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGct                                                                                                                         >  2:580946/1‑151 (MQ=255)                           gAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTc                                                                                                                    >  1:163502/1‑151 (MQ=255)                                   gATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACGGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCAttt                                                                                                            >  1:874136/1‑151 (MQ=255)                                    atttatttCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTg                                                                                                           >  2:538945/1‑151 (MQ=255)                                       tatttCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGcc                                                                                                         <  1:217341/150‑1 (MQ=255)                                              caGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGt                                                                                                  >  1:803497/1‑150 (MQ=255)                                                  gTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACa                                                                                             <  2:592775/151‑1 (MQ=255)                                                    aaCTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAgg                                                                                           <  2:473797/151‑1 (MQ=255)                                                    aaCTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAgg                                                                                           >  2:677227/1‑151 (MQ=255)                                                     aCTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggc                                                                                          <  1:704399/151‑1 (MQ=255)                                                       ttAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggcgg                                                                                        >  1:442376/1‑151 (MQ=255)                                                        tAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAggcgg                                                                                        <  1:868728/150‑1 (MQ=255)                                                         aaaTCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCGTTTGCCATTACGTAAACAGGCGGCa                                                                                      <  1:265913/151‑1 (MQ=255)                                                          aaTCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCa                                                                                      >  1:256748/1‑150 (MQ=255)                                                          aaTCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCa                                                                                      >  2:623364/1‑150 (MQ=255)                                                           aTCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCaa                                                                                     >  2:523930/1‑150 (MQ=255)                                                              aacaacGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAAc                                                                                  >  2:27366/1‑150 (MQ=255)                                                               acaacGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAATCAGGCGGCAAAACTc                                                                                <  2:276388/151‑1 (MQ=255)                                                                        agtaCAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACtgt                                                                        <  2:33459/150‑1 (MQ=255)                                                                        agtaCAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACtgt                                                                        <  1:427578/150‑1 (MQ=255)                                                                           aCAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCg                                                                     <  1:576579/150‑1 (MQ=255)                                                                           aCAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCg                                                                     >  2:614477/1‑150 (MQ=255)                                                                           aCAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGACCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGa                                                                    <  2:669940/151‑1 (MQ=255)                                                                            cAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGAt                                                                   >  1:856411/1‑151 (MQ=255)                                                                             aaTTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATc                                                                  >  2:758295/1‑151 (MQ=255)                                                                                 cGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCAt                                                               <  2:738396/150‑1 (MQ=255)                                                                                            cTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGc                                                    >  2:291684/1‑150 (MQ=255)                                                                                                     aaTTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGGGCAAGACGGcac                                          >  2:137193/1‑151 (MQ=255)                                                                                                      aTTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGcaca                                         <  1:452003/151‑1 (MQ=255)                                                                                                        tAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCacac                                        <  2:403712/150‑1 (MQ=255)                                                                                                         aGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACtt                                      <  2:226048/151‑1 (MQ=255)                                                                                                          gATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACttt                                     <  1:266006/151‑1 (MQ=255)                                                                                                          gATAAAGTACTGCTTCCCCTACAGGCAAGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACtt                                      <  1:575976/150‑1 (MQ=255)                                                                                                                  aCTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTcc                             >  1:138007/1‑151 (MQ=255)                                                                                                                     gCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCa                            <  1:898848/149‑1 (MQ=255)                                                                                                                       ttCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCAt                        >  1:262527/1‑151 (MQ=255)                                                                                                                        tCCCCTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCa                         <  1:889703/149‑1 (MQ=255)                                                                                                                         ccccTACAGGCAGGTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCAttt                      >  1:493471/1‑151 (MQ=255)                                                                                                                               caggcaggTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGtt                <  1:33127/151‑1 (MQ=255)                                                                                                                                  gcaggTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATc             <  2:407563/151‑1 (MQ=255)                                                                                                                                   caggTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCg            <  2:61186/151‑1 (MQ=255)                                                                                                                                     ggTAGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCa          <  1:689675/151‑1 (MQ=255)                                                                                                                                        aGCGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAAtg       >  1:742799/1‑151 (MQ=255)                                                                                                                                          cGAAGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAAtgtg     >  1:210067/1‑151 (MQ=255)                                                                                                                                             aGTCTTGCGTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGccc  >  1:902750/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                               GCACTCGTTACGCTCGCTTCGACCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTGTAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCTGTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCC  >  NC_000913/1898493‑1898783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑