Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,930,199	T→G	K64N (AAA→AAC)	yebF ←	extracellular Colicin M immunity family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,930,199	0	T	G	100.0%	203.3 / NA	52	K64N (AAA→AAC)	yebF	extracellular Colicin M immunity family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/28);  total (24/28)

CCGCCATTCCCGCTTTGCAGTCCACGCTGACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATTTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAGCGAAAACTGATGCGCAG  >  NC_000913/1930051‑1930346                                                                                                                                                     |                                                                                                                                                    ccGCCATTCCCGCTTTGCAGTCCACGCTGACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAAtgtt                                                                                                                                                   <  2:721016/151‑1 (MQ=255)  cGCCATTCCCGCTTTGCAGTCCACGCTGACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAAtgttt                                                                                                                                                  >  2:779357/1‑151 (MQ=255)      aTTCCCGCTTTGCAGTCCACGCTGACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGat                                                                                                                                              >  1:704036/1‑151 (MQ=255)                 gCAGTCCACGCTGACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCa                                                                                                                                    >  2:619658/1‑150 (MQ=255)                   aGTCCACGCTGACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAAcg                                                                                                                                 <  2:206075/151‑1 (MQ=255)                             gACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCAcgcg                                                                                                                        >  2:253457/1‑150 (MQ=255)                             gACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCAcgcg                                                                                                                        <  2:686376/150‑1 (MQ=255)                             gACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGa                                                                                                                       <  2:494177/151‑1 (MQ=255)                             gACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGa                                                                                                                       <  1:605629/151‑1 (MQ=255)                                 tGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGAtttt                                                                                                                   >  1:175381/1‑151 (MQ=255)                                     aaTGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTttgttg                                                                                                               >  2:469621/1‑151 (MQ=255)                                     aaTGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTttgtt                                                                                                                <  2:662470/150‑1 (MQ=255)                                     aaTGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTttgtt                                                                                                                <  2:921448/150‑1 (MQ=255)                                       tGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGAt                                                                                                             >  2:141689/1‑151 (MQ=255)                                        gAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATa                                                                                                            <  2:682819/151‑1 (MQ=255)                                         aaTATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATa                                                                                                            >  1:290839/1‑150 (MQ=255)                                            atCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCa                                                                                                        <  1:618041/151‑1 (MQ=255)                                                 cACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGttt                                                                                                   <  2:555713/151‑1 (MQ=255)                                                 cACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGttt                                                                                                   <  2:458664/151‑1 (MQ=255)                                                      ttACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACg                                                                                               <  1:672554/150‑1 (MQ=255)                                                       ttccACGCACGGTTAGCGGTACTGACCATTTATCATCTTTTCCCTGAATTGCCTGCAAACTGACCCAAGCCACTGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACg                                                                                               <  1:7086/147‑1 (MQ=255)                                                          cacgcacgGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTcgc                                                                                          <  1:908394/151‑1 (MQ=255)                                                            cgcacgGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCgg                                                                                        >  1:626562/1‑151 (MQ=255)                                                            cccacgGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTcgc                                                                                          <  2:601157/147‑1 (MQ=255)                                                             gcacgGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGc                                                                                       >  2:393752/1‑151 (MQ=255)                                                                 ggTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAAtt                                                                                   <  2:685340/151‑1 (MQ=255)                                                                   ttAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTcc                                                                                 >  2:517923/1‑151 (MQ=255)                                                                    tAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCg                                                                                >  1:560901/1‑151 (MQ=255)                                                                      gCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGgc                                                                              <  1:373212/151‑1 (MQ=255)                                                                        ggTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGgc                                                                              >  2:588239/1‑149 (MQ=255)                                                                              gACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATcc                                                                       <  2:738817/150‑1 (MQ=255)                                                                              gACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATcc                                                                       >  2:52838/1‑150 (MQ=255)                                                                              gACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATc                                                                        <  1:143490/149‑1 (MQ=255)                                                                                      atcatcTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTc                                                               >  1:917605/1‑150 (MQ=255)                                                                                         atcTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCacac                                                           >  1:274271/1‑151 (MQ=255)                                                                                          tcTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACt                                                          >  2:452995/1‑151 (MQ=255)                                                                                          tcTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACt                                                          >  2:481072/1‑151 (MQ=255)                                                                                              tACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTg                                                       <  1:437964/150‑1 (MQ=255)                                                                                                    gAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAg                                                 <  2:349896/150‑1 (MQ=255)                                                                                                             gCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACtt                                       >  1:525124/1‑151 (MQ=255)                                                                                                              cAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTg                                      >  1:520342/1‑151 (MQ=255)                                                                                                                 aCTGACCCAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTg                                   <  1:136789/151‑1 (MQ=255)                                                                                                                      cccAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTc                              <  1:670199/151‑1 (MQ=255)                                                                                                                        cAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCat                            >  1:104942/1‑151 (MQ=255)                                                                                                                        cAAGCCACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCat                            <  1:867870/151‑1 (MQ=255)                                                                                                                            ccACGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCattatt                        <  2:923061/151‑1 (MQ=255)                                                                                                                              aCGGGATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTgg                      <  1:790541/151‑1 (MQ=255)                                                                                                                                 ggATCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAg                   <  2:795486/151‑1 (MQ=255)                                                                                                                                   aTCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAGCg                 >  1:230221/1‑151 (MQ=255)                                                                                                                                    tCGGCCTGACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAGCg                 >  1:718118/1‑150 (MQ=255)                                                                                                                                           gACCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAGCGAAAACTGa         <  1:257432/151‑1 (MQ=255)                                                                                                                                            aCCGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAGCGAAAACTGAt        >  1:743674/1‑151 (MQ=255)                                                                                                                                              cGACAATGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAGCGAAAACTGATgc      >  2:101988/1‑151 (MQ=255)                                                                                                                                                  aaTGTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAGCGAAAACTGATGCgcag  <  1:765195/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CCGCCATTCCCGCTTTGCAGTCCACGCTGACCTGGTAATGAATATCGGCACTTTTACCACGCACGGTTAGCGGTACTGACCATTTATCATCTTTACCCTGAATGTCCTGCAAACTGACCCAAGCCACGGGATCGGCCTGACCGACAATTTTTTGATCATCTGCCCAACGCGCCACGCGATTTTGTTGATAATCACGTTTTACGCTCGCGGCAATTCCGGCAGCATCCAGATCTTCACACTTTGGGAAAGTGACCGACTTGCTGGTTTCATTATTGGCAGCGAAAACTGATGCGCAG  >  NC_000913/1930051‑1930346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑