Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	141,869	T→G	S147A (TCG→GCG)	hpt →	hypoxanthine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	141,869	0	T	G	100.0%	243.5 / NA	65	S147A (TCG→GCG)	hpt	hypoxanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/27);  total (38/27)

GTTGAAGATATCATCGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCGGCATGGCATTTTTATTTGT  >  NC_000913/141722‑142015                                                                                                                                                    |                                                                                                                                                   gttgAAGATATCATCGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTcgcg                                                                                                                                                  >  2:523979/1‑150 (MQ=255)   tgAAGATATCATCGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATc                                                                                                                                               >  2:603149/1‑151 (MQ=255)     aaGATATCATCGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATccc                                                                                                                                             <  2:585897/151‑1 (MQ=255)      aGATATCATCGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCg                                                                                                                                            >  2:88282/1‑151 (MQ=255)          atcatcGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATg                                                                                                                                        >  2:878874/1‑151 (MQ=255)          atcatcGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATg                                                                                                                                        >  1:792825/1‑151 (MQ=255)           tcatcGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATg                                                                                                                                        >  1:648094/1‑150 (MQ=255)             atcGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGt                                                                                                                                     >  1:190680/1‑151 (MQ=255)               cGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGttt                                                                                                                                   <  2:358819/151‑1 (MQ=255)                  cTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTtgtg                                                                                                                                >  1:787123/1‑151 (MQ=255)                    cGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTgtggt                                                                                                                              <  2:535574/151‑1 (MQ=255)                    cGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTgtggt                                                                                                                              <  2:543560/151‑1 (MQ=255)                     ggggAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTgtggtg                                                                                                                             >  1:308372/1‑151 (MQ=255)                     ggggAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTgtggtg                                                                                                                             >  1:68259/1‑151 (MQ=255)                         aaTACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGtt                                                                                                                         <  1:583101/151‑1 (MQ=255)                          aTACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTa                                                                                                                        <  2:383222/151‑1 (MQ=255)                            acacTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTAc                                                                                                                       <  1:217892/150‑1 (MQ=255)                            acacTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACg                                                                                                                      >  2:810573/1‑151 (MQ=255)                                tGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCAt                                                                                                                  >  2:117943/1‑151 (MQ=255)                                 gTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCAtt                                                                                                                 >  2:406803/1‑151 (MQ=255)                                 gTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCAt                                                                                                                  <  2:11056/150‑1 (MQ=255)                                            gTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGcac                                                                                                       >  1:446918/1‑150 (MQ=255)                                              gagaTCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGc                                                                                                    <  2:647703/151‑1 (MQ=255)                                               agaTCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGGGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCg                                                                                                   >  2:776128/1‑151 (MQ=255)                                                 aTCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGtt                                                                                                 >  1:531733/1‑151 (MQ=255)                                                     tAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGt                                                                                                  <  2:710980/146‑1 (MQ=255)                                                        gCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCa                                                                                          <  1:89804/151‑1 (MQ=255)                                                                       aGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGCAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGTTTACGCACAGCGGTACCTTCATCTGCCGTGTATCgg                                                                           >  2:651280/1‑151 (MQ=255)                                                                       aGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCgg                                                                           <  2:891790/151‑1 (MQ=255)                                                                          cGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCa                                                                         <  2:43608/150‑1 (MQ=255)                                                                          cGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCa                                                                         <  2:620715/150‑1 (MQ=255)                                                                             tGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAg                                                                      >  2:540231/1‑150 (MQ=255)                                                                                 gATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGAtt                                                                 <  1:603164/151‑1 (MQ=255)                                                                                 gATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGAtt                                                                 >  2:158944/1‑151 (MQ=255)                                                                                     tGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTctgc                                                             >  2:580677/1‑151 (MQ=255)                                                                                            tgctgGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACg                                                       >  1:709536/1‑150 (MQ=255)                                                                                            tgctgGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACg                                                       >  2:913822/1‑150 (MQ=255)                                                                                             gctgGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACg                                                       <  1:305402/149‑1 (MQ=255)                                                                                              ctgGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGt                                                    >  2:549885/1‑151 (MQ=255)                                                                                                ggATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGt                                                    <  1:885525/149‑1 (MQ=255)                                                                                                  aTAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAgt                                                <  1:522058/151‑1 (MQ=255)                                                                                                   tAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAgtg                                               >  1:84896/1‑151 (MQ=255)                                                                                                    aaaCCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAgtg                                               <  2:161361/150‑1 (MQ=255)                                                                                                      aCCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGtgtg                                             >  1:650785/1‑150 (MQ=255)                                                                                                      aCCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGtgtg                                             >  1:38357/1‑150 (MQ=255)                                                                                                       ccgtcccgtcGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGaa                                           <  2:282909/151‑1 (MQ=255)                                                                                                       ccgtcccgtcGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGa                                            >  1:897711/1‑150 (MQ=255)                                                                                                            ccgtcGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGtt                                        <  1:155221/149‑1 (MQ=255)                                                                                                              gtcgtGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCg                                    >  1:168129/1‑151 (MQ=255)                                                                                                               tcgtGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCgg                                   >  1:878346/1‑151 (MQ=255)                                                                                                               tcgtGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCgg                                   >  1:139467/1‑151 (MQ=255)                                                                                                                cgtGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGa                                  <  1:82572/151‑1 (MQ=255)                                                                                                                  tgaagtgaaCGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGAtg                                >  2:458693/1‑151 (MQ=255)                                                                                                                  tgaagggaaCGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCg                                    >  2:347417/1‑147 (MQ=255)                                                                                                                      gtgaaCGTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATgtgt                             <  2:373389/150‑1 (MQ=255)                                                                                                                            gTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATcc                      >  2:152330/1‑151 (MQ=255)                                                                                                                            gTCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATcc                      <  2:682503/151‑1 (MQ=255)                                                                                                                             tCCCGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCg                     >  2:411125/1‑151 (MQ=255)                                                                                                                                cGGTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCggc                   >  1:471697/1‑150 (MQ=255)                                                                                                                                  gTAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCggcatg                <  2:789249/151‑1 (MQ=255)                                                                                                                                   tAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCggcatgg               >  2:72529/1‑151 (MQ=255)                                                                                                                                   tAGAATTTATCGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCggcatgg               >  1:87568/1‑151 (MQ=255)                                                                                                                                             cGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCGGCATGGCATTTTTAtt     <  2:377036/151‑1 (MQ=255)                                                                                                                                             cGGTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCGGCATGGCATTTTTAt      <  2:307142/150‑1 (MQ=255)                                                                                                                                              ggTTTCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCGGCATGGCATTTTTAttt    >  1:912155/1‑151 (MQ=255)                                                                                                                                                tttCGCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCGGCATGGCATTTTTATTtgt  >  2:522335/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GTTGAAGATATCATCGACTCGGGGAATACACTGTCGAAAGTGCGTGAGATCTTAAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTACCGTCATCTGCCGTATATCGGCAAAGTGATTCTGCTGGACGAGTAAGTGTGAAGTTGCCGGATGTGTTGCATCCGGCATGGCATTTTTATTTGT  >  NC_000913/141722‑142015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑