| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,013,101 | T→G | intergenic (‑87/‑128) | fliE ← / → fliF | flagellar basal‑body component/flagellar basal‑body MS‑ring and collar protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,013,101 | 0 | T | G | 98.0% | 182.3 / ‑4.0 | 49 | intergenic (‑87/‑128) | fliE/fliF | flagellar basal‑body component/flagellar basal‑body MS‑ring and collar protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (20/28); minor base A (1/0); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01 | |||||||||||
GCACTCATCGCCGTAGCCTGTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTTATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGCGCGATGAATGCGACTGCAGCCC > NC_000913/2012953‑2013247 | gcacTCATCGCCGTAGCCTGTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGAt > 2:33489/1‑151 (MQ=255) cATCGCCGTAGCCTGTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCg > 2:115376/1‑151 (MQ=255) gCCGTAGCCTGTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGAt > 2:397084/1‑150 (MQ=255) gTAGCCTGTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑aa < 1:314004/151‑1 (MQ=255) cTGTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑aaaaaa > 1:492361/1‑150 (MQ=255) gTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAAc < 2:720472/150‑1 (MQ=255) gTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACg > 1:708829/1‑151 (MQ=255) aaCTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGa < 1:460468/150‑1 (MQ=255) ggCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAg < 2:666498/149‑1 (MQ=255) gCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGcac > 2:16428/1‑151 (MQ=255) aTAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACaa < 2:417149/149‑1 (MQ=255) aTAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAaat < 2:64793/151‑1 (MQ=255) aCCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAaataat > 2:840432/1‑151 (MQ=255) cAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCg > 2:672926/1‑151 (MQ=255) cAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCg < 1:592049/151‑1 (MQ=255) tCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTc > 1:373341/1‑150 (MQ=255) ccccTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCa < 1:190267/150‑1 (MQ=255) aTCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCg < 1:384893/151‑1 (MQ=255) gCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGt < 1:803764/151‑1 (MQ=255) gACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGttt < 1:922567/150‑1 (MQ=255) gACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGttt < 1:269630/150‑1 (MQ=255) gACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGttt < 1:274796/150‑1 (MQ=255) aCATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGc > 1:99160/1‑151 (MQ=255) cATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCt < 1:282250/151‑1 (MQ=255) cATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCt > 2:554967/1‑151 (MQ=255) cGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGAc < 2:165702/148‑1 (MQ=255) cccGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCggg < 2:745301/148‑1 (MQ=255) ccGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGgagtg < 2:815717/151‑1 (MQ=255) gATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGgagtgagt > 1:760610/1‑151 (MQ=255) aTAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTc < 1:703448/151‑1 (MQ=255) gCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTg < 2:747468/151‑1 (MQ=255) aaGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGATTGAGTCTTGTTCCACTTTGcc > 1:227683/1‑150 (MQ=255) aCCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAAc < 1:397093/150‑1 (MQ=255) ccAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGc < 2:778845/151‑1 (MQ=255) aGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCg < 1:518631/151‑1 (MQ=255) tAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAGAAAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGt > 1:843092/1‑151 (MQ=255) aTAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATc > 2:497312/1‑151 (MQ=255) aaGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATc > 1:866288/1‑149 (MQ=255) aCAAAGGCGCTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCa < 2:302770/151‑1 (MQ=255) gcgcTAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTTTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGt < 2:856984/151‑1 (MQ=255) tAAATAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTgcgc > 2:100403/1‑151 (MQ=255) aaaTAGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTgcg < 2:704321/149‑1 (MQ=255) aaaTAGCAACAAAAAAACGGGTTAATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGcgcg > 1:36031/1‑151 (MQ=255) aGCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGCGCGATGa < 1:143039/151‑1 (MQ=255) gCAACAAAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGCGCGATGaa < 1:440771/151‑1 (MQ=255) caaAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGCGCGATGAATGCg > 1:657641/1‑151 (MQ=255) caaAAAAACGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGCGCGATGAATGCg < 2:795941/151‑1 (MQ=255) aaCGGGTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGCGCGATGAATGCGACTGc > 1:760043/1‑150 (MQ=255) ggTTGATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGCGCGATGAATGCGACTGCAGccc > 2:480393/1‑151 (MQ=255) | GCACTCATCGCCGTAGCCTGTAACTGGCTGATAACCCCTTCAATCCCCTGTATCGCTGACATTCTCGTCTCCCGGATAATTTCTGGTAGCAAAGCCTACCAGTAAGTCAATAAGACAAAGGCGCTAAATAGCAACAAAAAAACGGGTTTATTGGCGGATAG‑AAAAAAACGAAAGCACAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCATAATCAGCCACGAGGTGCGCGATGAATGCGACTGCAGCCC > NC_000913/2012953‑2013247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |