Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,016,025	A→C	E53A (GAG→GCG)	fliH →	negative regulator of FliI ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,016,025	0	A	C	100.0%	190.7 / NA	53	E53A (GAG→GCG)	fliH	negative regulator of FliI ATPase activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/27);  total (26/27)

ATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGT  >  NC_000913/2015878‑2016172                                                                                                                                                    |                                                                                                                                                    aTCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATgcgc                                                                                                                                                   >  2:734613/1‑150 (MQ=255)     gCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAgg                                                                                                                                               <  1:738254/150‑1 (MQ=255)      ccGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGtt                                                                                                                                             <  1:735691/151‑1 (MQ=255)        gTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTAt                                                                                                                                           >  2:832591/1‑151 (MQ=255)          ggAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATc                                                                                                                                          >  2:425690/1‑150 (MQ=255)          ggAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATc                                                                                                                                          <  1:206362/150‑1 (MQ=255)          ggAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATc                                                                                                                                          <  1:826832/150‑1 (MQ=255)                ccTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCggg                                                                                                                                   >  2:279193/1‑151 (MQ=255)                ccTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCggg                                                                                                                                   <  1:919098/151‑1 (MQ=255)                   ggACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTAt                                                                                                                                >  1:611710/1‑151 (MQ=255)                         cGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGa                                                                                                                          <  1:810431/151‑1 (MQ=255)                          ggACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGa                                                                                                                          >  1:402013/1‑150 (MQ=255)                                   cgcgCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGt                                                                                                                      <  1:694185/145‑1 (MQ=255)                                             caGGCAGAGTGTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATa                                                                                                      <  2:795647/151‑1 (MQ=255)                                                    agTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAggg                                                                                               <  2:96079/151‑1 (MQ=255)                                                     gTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGc                                                                                              <  1:901096/151‑1 (MQ=255)                                                         gtgCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTAt                                                                                           <  2:155005/150‑1 (MQ=255)                                                               aTAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCaggaag                                                                                    <  1:822977/151‑1 (MQ=255)                                                               aTAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCATTAAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGCCGCCAGCATGGTCATAAGCAGGGCTTTCa                                                                                         >  2:607708/1‑146 (MQ=255)                                                                    cGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTg                                                                               >  1:671548/1‑151 (MQ=255)                                                                    cGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTg                                                                               >  1:223369/1‑151 (MQ=255)                                                                         cGGAAGAAACCATCATTGAAGAGGCTGAACCTAGCCTTAAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCa                                                                          <  2:69076/151‑1 (MQ=255)                                                                          ggAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGccc                                                                           >  2:567453/1‑149 (MQ=255)                                                                          ggAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAg                                                                         <  1:356557/151‑1 (MQ=255)                                                                              gaaACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCCGGAAGGACTGGCCCAgggg                                                                      >  2:815337/1‑150 (MQ=255)                                                                                   catcatTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCAATGCGCAAGGTTATCAGGCGGGGATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTgg                                                                  >  2:261151/1‑149 (MQ=255)                                                                                         tGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAgg                                                           <  2:788224/150‑1 (MQ=255)                                                                                          gAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGt                                                          <  1:673534/150‑1 (MQ=255)                                                                                          gAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGt                                                          <  2:885900/150‑1 (MQ=255)                                                                                          gAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTc                                                         <  2:515329/151‑1 (MQ=255)                                                                                                     aCCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                              <  2:804521/151‑1 (MQ=255)                                                                                                              tGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGtctc                                       >  1:865719/1‑149 (MQ=255)                                                                                                               gAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaac                                    >  2:440514/1‑151 (MQ=255)                                                                                                                agcagcAACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaac                                    >  1:296325/1‑150 (MQ=255)                                                                                                                 gcagcaACTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaaca                                   <  2:453587/150‑1 (MQ=255)                                                                                                                       aCTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGcc                             >  2:268283/1‑150 (MQ=255)                                                                                                                       aCTGGCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCa                            <  1:629485/151‑1 (MQ=255)                                                                                                                       aCTGGCGCAACTGCAAATGCAGGCACATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGcc                             >  2:187945/1‑150 (MQ=255)                                                                                                                          ggCGCAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAAtt                         <  2:722046/151‑1 (MQ=255)                                                                                                                            cgcAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTc                        >  1:647328/1‑150 (MQ=255)                                                                                                                            cgcAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCa                       <  2:708559/151‑1 (MQ=255)                                                                                                                             gcAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCAt                      <  1:159158/151‑1 (MQ=255)                                                                                                                             gcAACTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCAt                      <  2:402004/151‑1 (MQ=255)                                                                                                                               aaCTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGc                    >  2:349112/1‑151 (MQ=255)                                                                                                                                aCTGCAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGcc                   <  1:815227/151‑1 (MQ=255)                                                                                                                                    cAAATGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCgg                >  2:633112/1‑150 (MQ=255)                                                                                                                                     aaaTGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGAt              <  1:143750/151‑1 (MQ=255)                                                                                                                                     aaaTGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGAt              >  2:851220/1‑151 (MQ=255)                                                                                                                                        tGCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATgc            >  2:801129/1‑150 (MQ=255)                                                                                                                                         gCAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATgc            >  2:619867/1‑149 (MQ=255)                                                                                                                                          cAGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATgcagc         >  1:911282/1‑151 (MQ=255)                                                                                                                                           aGGCCCATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATgcag          >  2:405087/1‑149 (MQ=255)                                                                                                                                              cccATGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACt     >  2:588805/1‑151 (MQ=255)                                                                                                                                                 aTGCGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGt  >  1:862049/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATCTGCCGTGGAAAACCTGGACGCCGGACGATCTCGCGCCACCACAGGCAGAGTTTGTGCCCATAGTCGAGCCGGAAGAAACCATCATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGT  >  NC_000913/2015878‑2016172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑