Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,107,808	T→G	R174S (AGA→AGC)	glf ←	UDP‑galactopyranose mutase, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,107,808	0	T	G	100.0%	249.6 / NA	64	R174S (AGA→AGC)	glf	UDP‑galactopyranose mutase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/31);  total (33/31)

TTTACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGT  >  NC_000913/2107661‑2107949                                                                                                                                                    |                                                                                                                                              tttACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAgctc                                                                                                                                            >  2:596626/1‑151 (MQ=255)  ttACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAgctc                                                                                                                                            <  2:787356/150‑1 (MQ=255)     cTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTg                                                                                                                                        >  2:107526/1‑151 (MQ=255)       cGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTggg                                                                                                                                      >  2:23856/1‑151 (MQ=255)         cTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGAt                                                                                                                                    >  2:881624/1‑151 (MQ=255)            gagaATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCg                                                                                                                                 <  2:298922/151‑1 (MQ=255)             agaATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGc                                                                                                                                <  1:676062/151‑1 (MQ=255)              gaATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCt                                                                                                                               >  1:137733/1‑151 (MQ=255)               aaTCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCtt                                                                                                                              <  1:819070/151‑1 (MQ=255)               aaTCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCtt                                                                                                                              <  1:737027/151‑1 (MQ=255)                     tGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTtaataat                                                                                                                        >  2:418290/1‑151 (MQ=255)                        cTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTtaataataa                                                                                                                      >  2:460634/1‑150 (MQ=255)                        cTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTtaataataa                                                                                                                      >  1:836732/1‑150 (MQ=255)                              aaaaaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAgg                                                                                                               >  2:90090/1‑151 (MQ=255)                               aaaaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGc                                                                                                              <  1:692246/151‑1 (MQ=255)                               aaaaTCAATGCCTAATTTTACGTCCAAACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGc                                                                                                              >  2:219619/1‑151 (MQ=255)                                 aaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCa                                                                                                             >  2:570455/1‑150 (MQ=255)                                 aaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCa                                                                                                             >  2:280766/1‑150 (MQ=255)                                 aaTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCa                                                                                                             >  1:695027/1‑150 (MQ=255)                                  aTCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAAt                                                                                                           >  2:664030/1‑151 (MQ=255)                                     aaTGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCt                                                                                                        <  1:897388/151‑1 (MQ=255)                                        gCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCtttt                                                                                                     <  2:651628/151‑1 (MQ=255)                                        gCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCttt                                                                                                      <  2:623337/150‑1 (MQ=255)                                            aaTTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGc                                                                                                   <  1:766163/149‑1 (MQ=255)                                            aaTTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCAc                                                                                                 <  2:858658/151‑1 (MQ=255)                                             aTTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCAct                                                                                                <  2:828837/151‑1 (MQ=255)                                               tttACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCActt                                                                                               <  1:182235/150‑1 (MQ=255)                                                 tACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCActtct                                                                                             <  2:768943/150‑1 (MQ=255)                                                   cGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTcc                                                                                          <  1:913336/151‑1 (MQ=255)                                                      ccACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTcccc                                                                                        <  1:176671/150‑1 (MQ=255)                                                         cacCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACt                                                                                     <  2:503140/150‑1 (MQ=255)                                                         cacCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTg                                                                                    >  1:914865/1‑151 (MQ=255)                                                          acCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGc                                                                                   <  2:225193/151‑1 (MQ=255)                                                          acCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGc                                                                                   <  1:198819/151‑1 (MQ=255)                                                               cAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTtctc                                                                              <  1:681934/151‑1 (MQ=255)                                                                aaGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTcc                                                                             <  1:789612/151‑1 (MQ=255)                                                                  gCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCg                                                                            >  1:694976/1‑150 (MQ=255)                                                                      tttttCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGtata                                                                        >  1:29112/1‑150 (MQ=255)                                                                        tttCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAcc                                                                     >  1:621287/1‑151 (MQ=255)                                                                        tttCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTACCCACTGCTTCTCCGTATAAcc                                                                     >  2:249821/1‑151 (MQ=255)                                                                           cAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCtt                                                                  <  2:700366/151‑1 (MQ=255)                                                                             aTAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTa                                                                <  1:377726/151‑1 (MQ=255)                                                                               aaGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTAt                                                               <  2:416790/150‑1 (MQ=255)                                                                                        gtAGCCTCCCACCGGAATACCGTGAGAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTAGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCTCTTCTCCCCCACTGCTTCTCCGTATAACCCTTTACCAATGCTTg                                                      <  2:522580/150‑1 (MQ=255)                                                                                          aGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGta                                                   <  2:24963/151‑1 (MQ=255)                                                                                          aGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGt                                                    >  1:195280/1‑150 (MQ=255)                                                                                             cTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGtata                                                 >  1:98181/1‑150 (MQ=255)                                                                                               cccACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATaa                                                <  1:1802/149‑1 (MQ=255)                                                                                                            cTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTa                                  >  1:19521/1‑150 (MQ=255)                                                                                                             ttGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAAt                                >  1:379609/1‑151 (MQ=255)                                                                                                              tGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAAt                                <  1:3528/150‑1 (MQ=255)                                                                                                              tGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAAt                                <  2:190018/150‑1 (MQ=255)                                                                                                                   gCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATc                          >  1:333545/1‑151 (MQ=255)                                                                                                                   gCGATCGGAAAAATAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATc                          >  1:490346/1‑151 (MQ=255)                                                                                                                           aaaaaTAATTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGctc                  >  2:582761/1‑151 (MQ=255)                                                                                                                              aataatTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGctcctc               >  1:334221/1‑151 (MQ=255)                                                                                                                              aataatTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGctcctc               >  1:248782/1‑151 (MQ=255)                                                                                                                                taatTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCtcctcc              >  1:140571/1‑150 (MQ=255)                                                                                                                                 aatTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCa             <  1:317502/150‑1 (MQ=255)                                                                                                                                  atTGTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCaaa           <  2:599945/151‑1 (MQ=255)                                                                                                                                     gTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAAttt        >  1:166119/1‑151 (MQ=255)                                                                                                                                     gTTATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAAtt         <  1:237714/150‑1 (MQ=255)                                                                                                                                       tATCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTc      >  1:747162/1‑151 (MQ=255)                                                                                                                                        aTCAAACGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTc      >  1:180983/1‑150 (MQ=255)                                                                                                                                           aaaCGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGt  >  1:899142/1‑151 (MQ=255)                                                                                                                                            aaCGTAAAGCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGt  >  2:450663/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                              TTTACTCGCTAGAGAATCTTTGTCTTTCAAAAAATCAATGCCTAATTTTACGTCCACACCTTCAAGCATTTTTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAACCCTTTATCAATGCTTGGTATAAGTCCTCCCCAACTAATGAAATCGCCTGCTCCTCCAAATTTTCAGGT  >  NC_000913/2107661‑2107949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑