Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,206	A→C	F182V (TTT→GTT)	ompC ←	outer membrane porin protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,206	0	A	C	100.0%	205.8 / NA	54	F182V (TTT→GTT)	ompC	outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/24);  total (30/24)

CAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTC  >  NC_000913/2312059‑2312350                                                                                                                                                    |                                                                                                                                                 cagcGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGcc                                                                                                                                               >  1:221402/1‑151 (MQ=255)     ggTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAACCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCt                                                                                                                                              <  2:335138/148‑1 (MQ=255)            tGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATg                                                                                                                                    >  1:631253/1‑151 (MQ=255)             gAGCATCAGTACGTTTGGAGCGGGAGATCGCACCACCGATACCGAACCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATgg                                                                                                                                   <  2:57421/151‑1 (MQ=255)                cATCAGTACGTTTGGAGCTGGAGATCGCACCCCCGATCCCGAACCCTTCGTAATCATAGGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGt                                                                                                                                 <  1:603269/150‑1 (MQ=255)                  tCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGc                                                                                                                              >  2:248027/1‑151 (MQ=255)                      tACGTTTGGAGCTGGAGATCGCACCCCCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGt                                                                                                                           <  1:127026/150‑1 (MQ=255)                       aCGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGttt                                                                                                                         >  2:707714/1‑151 (MQ=255)                       aCGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGttt                                                                                                                         >  2:505235/1‑151 (MQ=255)                        cGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGtttt                                                                                                                        >  2:618677/1‑151 (MQ=255)                         gTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGttttt                                                                                                                       >  1:492021/1‑151 (MQ=255)                         gTTTGGAGCGGGAGATCGCCCCACCGATACCGAACCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGttt                                                                                                                         <  1:820465/149‑1 (MQ=255)                            tGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTAcc                                                                                                                    >  2:298957/1‑151 (MQ=255)                                 cTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGt                                                                                                               >  2:908176/1‑151 (MQ=255)                                  tGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGt                                                                                                               >  2:824788/1‑150 (MQ=255)                                   ggAGATCGCACCACCGATACCGAACCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTa                                                                                                              <  1:680975/150‑1 (MQ=255)                                       aTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGa                                                                                                          >  1:703593/1‑150 (MQ=255)                                             ccaccGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCa                                                                                                    >  2:920715/1‑150 (MQ=255)                                                ccGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg                                                                                                 >  1:453798/1‑150 (MQ=255)                                                 cGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt                                                                                               >  1:305311/1‑151 (MQ=255)                                                  gATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTc                                                                                              >  1:526601/1‑151 (MQ=255)                                                  gATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTc                                                                                              >  2:423813/1‑151 (MQ=255)                                                     aCCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg                                                                                           >  2:194514/1‑151 (MQ=255)                                                      ccGAACCTTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAg                                                                                            <  2:539895/149‑1 (MQ=255)                                                        gAAACCTTGGTATTCATAAGGGAGAGACCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa                                                                                                         <  2:492767/134‑1 (MQ=255)                                                        gAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCg                                                                                        >  1:158890/1‑151 (MQ=255)                                                              ttCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAcc                                                                                  >  1:34960/1‑151 (MQ=255)                                                                 gTAATCATAGGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                 <  1:618564/149‑1 (MQ=255)                                                                 gTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAcc                                                                                  >  2:507216/1‑148 (MQ=255)                                                                      cATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCga                                                                           >  1:49249/1‑150 (MQ=255)                                                                                aGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtg                                                                >  2:453095/1‑151 (MQ=255)                                                                                  aaCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGtt                                                              <  1:77998/151‑1 (MQ=255)                                                                                   aCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa                                                             <  2:597211/151‑1 (MQ=255)                                                                                       ccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGt                                                         >  1:628689/1‑151 (MQ=255)                                                                                        cgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTa                                                        <  2:719105/151‑1 (MQ=255)                                                                                         gACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGt                                                         >  1:44702/1‑149 (MQ=255)                                                                                                    cgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc                                            <  1:507230/151‑1 (MQ=255)                                                                                                     gtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg                                           <  2:650474/151‑1 (MQ=255)                                                                                                        ttGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt                                         <  2:56063/150‑1 (MQ=255)                                                                                                         tGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTa                                        <  1:160324/150‑1 (MQ=255)                                                                                                          gACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAcc                                      <  2:492008/151‑1 (MQ=255)                                                                                                               aGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgct                                 >  1:618893/1‑151 (MQ=255)                                                                                                               aGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgct                                 <  2:120639/151‑1 (MQ=255)                                                                                                                  gCGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgc                               <  2:359056/150‑1 (MQ=255)                                                                                                                   cGTCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGctgctg                             <  2:142543/151‑1 (MQ=255)                                                                                                                     tCACGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCtgctgc                            >  2:10831/1‑150 (MQ=255)                                                                                                                        cGACCGTTGTTAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGa                        <  2:366352/151‑1 (MQ=255)                                                                                                                               tgttAGTTACGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt                  <  1:95634/150‑1 (MQ=255)                                                                                                                                 ttagttaCGCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTc                 >  2:375183/1‑149 (MQ=255)                                                                                                                                         gCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGt         >  2:37616/1‑149 (MQ=255)                                                                                                                                         gCCACTAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAg       <  1:824806/151‑1 (MQ=255)                                                                                                                                              tAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGt         >  2:534497/1‑144 (MQ=255)                                                                                                                                              tAGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGt         <  1:534512/144‑1 (MQ=255)                                                                                                                                               aGTAACGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTc  >  1:362859/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTC  >  NC_000913/2312059‑2312350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑