Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,348,730	T→G	C73G (TGT→GGT)	yfaE →	ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,348,730	0	T	G	100.0%	209.0 / NA	54	C73G (TGT→GGT)	yfaE	ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/33);  total (21/33)

GGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTG  >  NC_000913/2348582‑2348874                                                                                                                                                     |                                                                                                                                                 ggcggcGCTGGAATCCCACAATGTGGCGGTTGGGTAACAAAGTCGGGAAGGGTAATTCGGCGACTGTCGCAAAAGCCGGGGTGCAGGTCAAGTTGGCTGGATTTCCCGACCGTTTACCTTTATTTAGCCGGGGGGAATTTTGCCCtgttgt                                                                                                                                                >  1:602380/1‑151 (MQ=255)       gcTGGAATCCCACAATGTGGCGTTTGAGTACCAGTTTCTCGAAGGTTACTGCGGCTCCTGTCTCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCgg                                                                                                                                          <  2:37791/151‑1 (MQ=255)                   caATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCCCACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGa                                                                                                                               <  2:791695/150‑1 (MQ=255)                        tgGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTg                                                                                                                          >  2:119845/1‑150 (MQ=255)                         ggCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGa                                                                                                                         <  2:157154/150‑1 (MQ=255)                         ggCGGTTGAGTACCAGTGCCGCGAAGGTTACTGCGGCGCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGatat                                                                                                                            <  2:283818/147‑1 (MQ=255)                          gCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGaaa                                                                                                                       <  2:73157/151‑1 (MQ=255)                            ggTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATc                                                                                                                     >  2:132773/1‑151 (MQ=255)                             gTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCg                                                                                                                    >  2:486714/1‑151 (MQ=255)                              ttGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCg                                                                                                                    >  1:279759/1‑150 (MQ=255)                               tGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCga                                                                                                                   <  1:50131/150‑1 (MQ=255)                                gAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCgag                                                                                                                  <  1:772003/150‑1 (MQ=255)                                  gTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGAtg                                                                                                               <  1:244057/151‑1 (MQ=255)                                   tACCAGTGTCGCGAAGGTTGCTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGAtg                                                                                                               <  2:854055/150‑1 (MQ=255)                                   tACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGAtgt                                                                                                              >  1:417500/1‑151 (MQ=255)                                     ccAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGAtgtg                                                                                                             <  2:756374/150‑1 (MQ=255)                                      cAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGa                                                                                                            >  1:589333/1‑150 (MQ=255)                                        gtgtCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAAtt                                                                                                         <  2:352782/151‑1 (MQ=255)                                            cgcgAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGtt                                                                                                     <  1:501698/151‑1 (MQ=255)                                            cgcgAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGt                                                                                                      <  2:668329/150‑1 (MQ=255)                                               gAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGt                                                                                                   >  1:554478/1‑150 (MQ=255)                                                aaGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAg                                                                                                 <  2:353573/151‑1 (MQ=255)                                                         gCGGTTCCTTGCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCATTTATTCAGCCGGGGGAAATTTTGGCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCGGAt                                                                                         <  2:43314/150‑1 (MQ=255)                                                         gCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGa                                                                                          <  2:538312/149‑1 (MQ=255)                                                         gCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGAt                                                                                         <  1:132410/150‑1 (MQ=255)                                                               ccTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGc                                                                                  <  1:164009/151‑1 (MQ=255)                                                                  gTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCtt                                                                                <  2:871202/150‑1 (MQ=255)                                                                                gTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTgg                                                                 <  1:801149/151‑1 (MQ=255)                                                                                 ttGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                >  1:201346/1‑151 (MQ=255)                                                                                  tGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGcc                                                               >  2:35334/1‑151 (MQ=255)                                                                                   gCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGcc                                                               <  1:787178/150‑1 (MQ=255)                                                                                   gCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGcc                                                               <  1:623825/150‑1 (MQ=255)                                                                                   gCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCt                                                              <  2:570690/151‑1 (MQ=255)                                                                                      gctCAAGTTGACTGGATTTCCGAACCGTTAGCCTTTATTCAGCCTGGGTAAATTTTGCCCTGTGGTTGCCGTGCAAAAGCCGATATTGAAATCGAGATGTGAATTGGTTGTATTGCCAGATACAACGCTTATGCGTCTTATCTAGCCTACa                                                           <  2:127909/149‑1 (MQ=255)                                                                                       gTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACaa                                                          <  1:237186/151‑1 (MQ=255)                                                                                        tCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAAc                                                         >  1:565485/1‑151 (MQ=255)                                                                                         cAAGTTTACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGTTTATGCGTCTTATCTGGCCTACAACg                                                        <  2:641623/151‑1 (MQ=255)                                                                                           aGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGAt                                                      >  1:419932/1‑151 (MQ=255)                                                                                            gTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGAtt                                                     >  2:548123/1‑151 (MQ=255)                                                                                            gTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGAt                                                      <  2:142436/150‑1 (MQ=255)                                                                                                     aTTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgt                                            <  2:856129/151‑1 (MQ=255)                                                                                                      ttGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgt                                            >  1:285143/1‑150 (MQ=255)                                                                                                        gCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCgtag                                          <  2:636461/150‑1 (MQ=255)                                                                                                          cGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTaa                                       <  1:72270/151‑1 (MQ=255)                                                                                                              ccGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACg                                   >  1:758496/1‑151 (MQ=255)                                                                                                               cGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGc                                  >  2:206866/1‑151 (MQ=255)                                                                                                               cGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGc                                  >  1:50537/1‑151 (MQ=255)                                                                                                                  tAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCttt                               >  1:28757/1‑151 (MQ=255)                                                                                                                       tttATTCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCt                          >  1:455710/1‑151 (MQ=255)                                                                                                                            tCAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTcc                     >  2:82113/1‑151 (MQ=255)                                                                                                                             cAGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTcc                     <  1:119846/150‑1 (MQ=255)                                                                                                                              aGCCGGGGGAAATTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTccc                    >  1:591380/1‑150 (MQ=255)                                                                                                                                       aaaTTTTGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCttt          <  1:839354/151‑1 (MQ=255)                                                                                                                                            ttGCCCTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgggg      >  1:75157/1‑150 (MQ=255)                                                                                                                                               cccTGTGGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTg  <  1:42528/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTG  >  NC_000913/2348582‑2348874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑