Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,356,017	T→G	F166C (TTT→TGT)	glpC →	anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,356,017	0	T	G	100.0%	288.8 / NA	75	F166C (TTT→TGT)	glpC	anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/39);  total (36/39)

CGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAA  >  NC_000913/2355870‑2356161                                                                                                                                                    |                                                                                                                                                 cGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCa                                                                                                                                               <  2:736062/151‑1 (MQ=255)      aaaCCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGgct                                                                                                                                          <  2:880028/151‑1 (MQ=255)            gTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCg                                                                                                                                    >  1:901667/1‑151 (MQ=255)               cGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTa                                                                                                                                 <  2:183167/151‑1 (MQ=255)                    gctgctTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTAc                                                                                                                            <  2:93807/151‑1 (MQ=255)                     ctgctTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTAc                                                                                                                            >  1:720120/1‑150 (MQ=255)                       gctTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACa                                                                                                                           <  1:875756/149‑1 (MQ=255)                       gctTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAAc                                                                                                                         <  1:663958/151‑1 (MQ=255)                          tGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCAt                                                                                                                      >  1:454123/1‑151 (MQ=255)                          tGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCAt                                                                                                                      >  1:489799/1‑151 (MQ=255)                              gcggcgTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCg                                                                                                                   <  1:896861/150‑1 (MQ=255)                              gcggcgTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGc                                                                                                                  <  2:775956/151‑1 (MQ=255)                                  cgTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGtt                                                                                                              <  2:192730/151‑1 (MQ=255)                                  cgTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCAGCAGtt                                                                                                              <  2:89746/151‑1 (MQ=255)                                     tAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAg                                                                                                            <  1:161672/150‑1 (MQ=255)                                     tAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAg                                                                                                            <  1:760762/150‑1 (MQ=255)                                        aaaTCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTaa                                                                                                        <  2:201647/151‑1 (MQ=255)                                            cGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGa                                                                                                     <  2:452121/150‑1 (MQ=255)                                              atcatcGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGAtt                                                                                                   <  2:528625/150‑1 (MQ=255)                                               tcatcGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGAttt                                                                                                  >  2:128489/1‑150 (MQ=255)                                                 atcGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCATGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATttaat                                                                                               >  2:817851/1‑151 (MQ=255)                                                 atcGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATttaat                                                                                               <  1:369911/151‑1 (MQ=255)                                                 atcGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATttaat                                                                                               >  1:855681/1‑151 (MQ=255)                                                   cgccgcACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATttaatta                                                                                             <  2:864072/151‑1 (MQ=255)                                                   cgccgcACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATttaatt                                                                                              >  1:699240/1‑150 (MQ=255)                                                      cgcACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAg                                                                                          <  1:212564/151‑1 (MQ=255)                                                         aCGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGc                                                                                       >  2:301751/1‑151 (MQ=255)                                                           gCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTc                                                                                     >  1:693653/1‑151 (MQ=255)                                                             tACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTc                                                                                     >  2:487910/1‑149 (MQ=255)                                                              aCCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCaa                                                                                   <  1:95168/150‑1 (MQ=255)                                                                cGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGc                                                                                >  2:407935/1‑151 (MQ=255)                                                                 gAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGc                                                                                >  2:13660/1‑150 (MQ=255)                                                                   aaTACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAAt                                                                             <  1:565358/151‑1 (MQ=255)                                                                   aaTACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAAt                                                                             >  1:400542/1‑151 (MQ=255)                                                                   aaTACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAAt                                                                             <  2:514701/151‑1 (MQ=255)                                                                      aCTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATggg                                                                          >  1:773179/1‑151 (MQ=255)                                                                      aCTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATggg                                                                          >  1:309197/1‑151 (MQ=255)                                                                      aCTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATggg                                                                          >  2:800671/1‑151 (MQ=255)                                                                         ccTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTa                                                                        <  2:722210/150‑1 (MQ=255)                                                                         ccTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTAc                                                                       <  2:11980/151‑1 (MQ=255)                                                                         ccTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTAc                                                                       >  1:104094/1‑151 (MQ=255)                                                                          cTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTAcc                                                                      >  1:31752/1‑151 (MQ=255)                                                                           ttCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCg                                                                     >  2:282837/1‑151 (MQ=255)                                                                            tCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCgg                                                                    >  1:636974/1‑151 (MQ=255)                                                                             cGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGgt                                                                   <  2:534077/151‑1 (MQ=255)                                                                                cACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGgtgt                                                                 >  1:629388/1‑150 (MQ=255)                                                                                 aCGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTAc                                                               >  2:631958/1‑151 (MQ=255)                                                                                 aCGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTAc                                                               <  2:96224/151‑1 (MQ=255)                                                                                   gTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACa                                                              <  2:165417/150‑1 (MQ=255)                                                                                     tCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACt                                                           >  2:904793/1‑151 (MQ=255)                                                                                      ccGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTg                                                          <  1:652039/151‑1 (MQ=255)                                                                                        gTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCt                                                        >  2:617186/1‑151 (MQ=255)                                                                                          cGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCa                                                      <  2:368387/151‑1 (MQ=255)                                                                                           gCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAg                                                     <  1:61471/151‑1 (MQ=255)                                                                                             tGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGc                                                    >  1:562887/1‑150 (MQ=255)                                                                                              ggTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCa                                                   <  2:404999/150‑1 (MQ=255)                                                                                                 aTCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGa                                               <  2:715765/151‑1 (MQ=255)                                                                                                     cAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGaaaaa                                           <  2:239017/151‑1 (MQ=255)                                                                                                     cAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGaaaa                                            >  2:777845/1‑150 (MQ=255)                                                                                                      aGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAAt                                          >  2:307063/1‑151 (MQ=255)                                                                                                      aGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAAt                                          >  1:647075/1‑151 (MQ=255)                                                                                                             cggcTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTgcgg                                   >  2:596748/1‑151 (MQ=255)                                                                                                             cggcTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTgcgg                                   >  1:70819/1‑151 (MQ=255)                                                                                                                  cAGCAAGCACAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTa                               <  1:458379/150‑1 (MQ=255)                                                                                                                   agcaagcaCAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTa                               <  1:641462/149‑1 (MQ=255)                                                                                                                   agcaagcaCAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTAcc                             <  1:850656/151‑1 (MQ=255)                                                                                                                     caagcaCAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGc                           >  2:735201/1‑151 (MQ=255)                                                                                                                     caagcaCAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGc                           >  2:202425/1‑151 (MQ=255)                                                                                                                        gcaCAATATAAAGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTa                         >  1:736257/1‑150 (MQ=255)                                                                                                                                 aaaGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAAc                <  2:451542/150‑1 (MQ=255)                                                                                                                                 aaaGACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACg               >  2:207675/1‑151 (MQ=255)                                                                                                                                    gACCAGGTCGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCt            >  2:704702/1‑151 (MQ=255)                                                                                                                                            cGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGa     <  2:908355/150‑1 (MQ=255)                                                                                                                                            cGCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGAt    <  1:407944/151‑1 (MQ=255)                                                                                                                                             gCTTTCTGTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATa   <  2:844167/151‑1 (MQ=255)                                                                                                                                                ttctgtcACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATaa  >  1:699005/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAA  >  NC_000913/2355870‑2356161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑