Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,376,074	A→C	L249R (CTG→CGG)	menB ←	dihydroxynaphthoic acid synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,376,074	0	A	C	100.0%	144.6 / NA	45	L249R (CTG→CGG)	menB	dihydroxynaphthoic acid synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/14);  total (31/14)

TGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGTACCACGGTGTTC  >  NC_000913/2375926‑2376217                                                                                                                                                     |                                                                                                                                                tGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCccgcccgc                                                                                                                                               >  2:10624/1‑151 (MQ=255)  ggggATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCTTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCccgcccgc                                                                                                                                               <  2:588484/150‑1 (MQ=255)            cAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGccc                                                                                                                                    >  2:408586/1‑151 (MQ=255)                    tcccTGCGCGCTACGCATTACGGTTCCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGCCCTCCCGGCCCTTCTCCCGTCATGTAGAACAGCATGGTGGCTTGCCCCGCCCGCTCCTGCAGCCCCGCCTGc                                                                                                                             <  2:675027/148‑1 (MQ=255)                    taCCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGcc                                                                                                                            >  1:514010/1‑151 (MQ=255)                      ccTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCg                                                                                                                          >  2:588552/1‑151 (MQ=255)                            cgcTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTcac                                                                                                                      >  1:763682/1‑149 (MQ=255)                               tACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCg                                                                                                                 >  1:113305/1‑151 (MQ=255)                                 cGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGc                                                                                                               >  1:449276/1‑151 (MQ=255)                                     ttACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTc                                                                                                           >  2:383195/1‑151 (MQ=255)                                      tACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTc                                                                                                           <  1:734032/150‑1 (MQ=255)                                      tACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTc                                                                                                           >  1:724757/1‑150 (MQ=255)                                           aTTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGc                                                                                                      >  2:541014/1‑150 (MQ=255)                                           aTTCCGTTTGAATTTGCTGAAGTCACGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTCCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGc                                                                                                      >  1:898306/1‑150 (MQ=255)                                            ttCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGc                                                                                                      >  1:303846/1‑149 (MQ=255)                                             tCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGc                                                                                                   >  2:924411/1‑151 (MQ=255)                                                      aTTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGgc                                                                                           >  1:304390/1‑150 (MQ=255)                                                           cTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGgcagcgc                                                                                      >  1:162964/1‑150 (MQ=255)                                                             gAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGCCCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGgcagcgca                                                                                     <  2:261774/149‑1 (MQ=255)                                                             gAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGgcagcgcagc                                                                                   >  1:68906/1‑151 (MQ=255)                                                               aGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCagcgcggcgc                                                                                 >  2:222574/1‑151 (MQ=255)                                                                  cAGGCTGACGTTTCTGGTTGAAGGCGTTGCGCCCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCACGCATCGCAGCGCCa                                                                               >  2:209835/1‑150 (MQ=255)                                                                      cTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCgg                                                                           <  1:410088/150‑1 (MQ=255)                                                                       tGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGc                                                                         >  1:524587/1‑151 (MQ=255)                                                                        gACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCt                                                                        >  1:783341/1‑151 (MQ=255)                                                                         aCGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGCCCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTg                                                                       <  2:203338/151‑1 (MQ=255)                                                                             ttCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGtttt                                                                   >  2:278475/1‑151 (MQ=255)                                                                              tCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGtttt                                                                   <  2:25985/150‑1 (MQ=255)                                                                               cTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTgc                                                                 >  1:99224/1‑151 (MQ=255)                                                                                  gTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTgcagc                                                              >  1:70156/1‑151 (MQ=255)                                                                                    tGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCAt                                                            >  1:764407/1‑151 (MQ=255)                                                                                        ggCGTTGCGCCCTTCCTGCCCTTCTCCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTcg                                                        <  2:189571/151‑1 (MQ=255)                                                                                               cgcccTTCCTGCCCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCAc                                                  <  1:114760/147‑1 (MQ=255)                                                                                                   cTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAacg                                             >  2:516479/1‑151 (MQ=255)                                                                                                      ccTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCAGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAacggac                                          >  1:480620/1‑151 (MQ=255)                                                                                                        tGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAacggacgg                                        >  2:326319/1‑151 (MQ=255)                                                                                                           ccTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGttt                                     >  2:778810/1‑151 (MQ=255)                                                                                                           ccTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGttt                                     >  2:362102/1‑151 (MQ=255)                                                                                                           ccTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGtt                                      <  1:11116/150‑1 (MQ=255)                                                                                                            cttcttCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGttt                                     >  1:628969/1‑150 (MQ=255)                                                                                                                         gTAGAACAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATc                        <  1:512592/150‑1 (MQ=255)                                                                                                                             accAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCa                   <  1:761689/149‑1 (MQ=255)                                                                                                                             aaCAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCa                   <  1:588567/151‑1 (MQ=255)                                                                                                                               cAGCATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAg                  <  1:819073/150‑1 (MQ=255)                                                                                                                                  cATGGTGGCGTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGt              >  2:523006/1‑151 (MQ=255)                                                                                                                                           gTTGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGTACCACGgtg     >  2:388694/1‑151 (MQ=255)                                                                                                                                            ttGCCCGCCCGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGTACCACGgt      <  1:58962/149‑1 (MQ=255)                                                                                                                                               cccgcccgCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGTACCACGGTGTTc  <  1:582739/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                TGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATGTAGAACAGCATGGTGGCGTTGCCCGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGTACCACGGTGTTC  >  NC_000913/2375926‑2376217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑