Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,380,295	A→C	C117G (TGT→GGT)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,380,295	0	A	C	100.0%	182.2 / NA	48	C117G (TGT→GGT)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/16);  total (32/16)

TGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCC  >  NC_000913/2380150‑2380442                                                                                                                                                  |                                                                                                                                                    tgttgtTCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTc                                                                                                                                                >  2:798825/1‑151 (MQ=255)  gttgttCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCg                                                                                                                                               <  1:715840/151‑1 (MQ=255)     gttCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCa                                                                                                                                            <  2:563029/151‑1 (MQ=255)          cgcgTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTcc                                                                                                                                        <  2:621344/150‑1 (MQ=255)            cgTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAgg                                                                                                                                     <  1:631294/151‑1 (MQ=255)             gtggtgGTTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGc                                                                                                                                    <  1:4525/151‑1 (MQ=255)               ggtggtTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCg                                                                                                                                   <  1:724016/150‑1 (MQ=255)                 tggtTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCgggg                                                                                                                                >  1:428650/1‑151 (MQ=255)                    ttAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTa                                                                                                                              >  2:844547/1‑150 (MQ=255)                      aaaTGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTaaa                                                                                                                            >  1:839212/1‑150 (MQ=255)                          gTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTaa                                                                                                                       <  2:642773/151‑1 (MQ=255)                              cccAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTg                                                                                                                   >  2:848757/1‑151 (MQ=255)                               ccAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGc                                                                                                                  >  1:314394/1‑151 (MQ=255)                               ccAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGc                                                                                                                  >  2:634237/1‑151 (MQ=255)                                     aaGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCTTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGc                                                                                                             <  1:159813/150‑1 (MQ=255)                                     aaGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCg                                                                                                            >  1:212692/1‑151 (MQ=255)                                             gATACTCTCCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCa                                                                                                    <  1:225995/151‑1 (MQ=255)                                               tACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCaa                                                                                                   >  1:310415/1‑150 (MQ=255)                                                aCTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCaaa                                                                                                  <  2:444972/150‑1 (MQ=255)                                                aCTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCaaa                                                                                                  >  2:802577/1‑150 (MQ=255)                                                aCTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGGAAATTGCCCTGCGACGGGTCaaa                                                                                                  >  2:689238/1‑150 (MQ=255)                                                 cTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCaaa                                                                                                  <  1:315504/149‑1 (MQ=255)                                                            ggCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGcccc                                                                                     >  1:351404/1‑151 (MQ=255)                                                                  aaaTTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATg                                                                               >  1:38668/1‑151 (MQ=255)                                                                     ttCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGt                                                                            >  2:161587/1‑151 (MQ=255)                                                                      tCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGt                                                                            >  1:804000/1‑150 (MQ=255)                                                                       cTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAACCCCCAAATGCGTa                                                                           >  1:729993/1‑150 (MQ=255)                                                                        ttttGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTa                                                                           >  1:379447/1‑149 (MQ=255)                                                                              cTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCg                                                                   >  2:304548/1‑151 (MQ=255)                                                                               tGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCgt                                                                  >  1:920454/1‑151 (MQ=255)                                                                                      cATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCgg                                                           >  1:247912/1‑151 (MQ=255)                                                                                         cGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGt                                                         <  1:26624/150‑1 (MQ=255)                                                                                            gCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTg                                                     >  1:484875/1‑151 (MQ=255)                                                                                                   ggAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaaga                                              <  2:321810/151‑1 (MQ=255)                                                                                                    gAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaaga                                              >  1:559287/1‑150 (MQ=255)                                                                                                             cTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTtgtg                                    >  2:903802/1‑151 (MQ=255)                                                                                                                  tAGCGTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTgg                               >  1:26109/1‑151 (MQ=255)                                                                                                                      gTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTcc                            >  1:48008/1‑150 (MQ=255)                                                                                                                      gTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCa                           <  1:798843/151‑1 (MQ=255)                                                                                                                      gTCAGCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCa                           <  1:610551/151‑1 (MQ=255)                                                                                                                          gCCGCAGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAacg                        >  2:291819/1‑150 (MQ=255)                                                                                                                               aGCGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTa                   >  1:581008/1‑150 (MQ=255)                                                                                                                                 cGTGGCTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTaaa                 >  1:278972/1‑150 (MQ=255)                                                                                                                                      cTTTACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACggg            >  2:337984/1‑150 (MQ=255)                                                                                                                                        ttACCGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTa          >  1:554484/1‑150 (MQ=255)                                                                                                                                            cGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATcgc     <  2:244464/151‑1 (MQ=255)                                                                                                                                            cGCCACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATcg      >  2:895371/1‑150 (MQ=255)                                                                                                                                              ccACCGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCgcgc   >  2:382581/1‑151 (MQ=255)                                                                                                                                               caccGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGcc  <  2:212689/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TGTTGTTCTCGCGTGGTGGTTAAATGTAACCCAGGCAAGGGCTTGATACTCACCAGTGTGGCGATAAATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCC  >  NC_000913/2380150‑2380442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑