Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,492,875 A→C F127C (TTT→TGT)  frc ← formyl‑CoA transferase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,492,8750AC98.5% 248.5 / ‑3.0 67F127C (TTT→TGT) frcformyl‑CoA transferase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (24/42);  total (25/42)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01

AATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCT  >  NC_000913/2492729‑2493021
                                                                                                                                                  |                                                                                                                                                  
aaTGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACAccc                                                                                                                                                >  2:137440/1‑151 (MQ=255)
   gCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCttt                                                                                                                                             <  1:499167/151‑1 (MQ=255)
   gCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCttt                                                                                                                                             <  1:464464/151‑1 (MQ=255)
   gCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCtt                                                                                                                                              <  2:151304/150‑1 (MQ=255)
    cATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTg                                                                                                                                            <  2:806090/151‑1 (MQ=255)
    cATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTg                                                                                                                                            >  1:311787/1‑151 (MQ=255)
            tgtTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAAcc                                                                                                                                    >  1:391322/1‑151 (MQ=255)
             gtTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAAcc                                                                                                                                    <  2:45255/150‑1 (MQ=255)
                  tGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAAt                                                                                                                              >  1:520150/1‑151 (MQ=255)
                   gTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATc                                                                                                                             <  2:437007/151‑1 (MQ=255)
                       cccAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGAc                                                                                                                         <  1:827102/151‑1 (MQ=255)
                           aCGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCATACGTgg                                                                                                                     <  2:470488/151‑1 (MQ=255)
                           aCGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTgg                                                                                                                     <  1:717943/151‑1 (MQ=255)
                               tGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGgattg                                                                                                                 >  2:560800/1‑151 (MQ=255)
                                  aGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGgattgatt                                                                                                              <  2:354983/151‑1 (MQ=255)
                                   gcgcTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGAttt                                                                                                             <  1:373735/151‑1 (MQ=255)
                                        tACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTg                                                                                                         <  2:340809/150‑1 (MQ=255)
                                           cAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAata                                                                                                     <  1:326926/151‑1 (MQ=255)
                                           cAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAata                                                                                                     <  2:839796/151‑1 (MQ=255)
                                           cAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAat                                                                                                      >  1:10108/1‑150 (MQ=255)
                                            aGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAatat                                                                                                    <  2:788643/151‑1 (MQ=255)
                                                  ggACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTccc                                                                                              <  2:754431/151‑1 (MQ=255)
                                                   gACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCa                                                                                             <  1:801542/151‑1 (MQ=255)
                                                    aCCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAg                                                                                            <  2:305114/151‑1 (MQ=255)
                                                      cATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGt                                                                                          <  1:778052/151‑1 (MQ=255)
                                                      cATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGt                                                                                          <  1:115954/151‑1 (MQ=255)
                                                         cccAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGa                                                                                        >  2:377463/1‑150 (MQ=255)
                                                           cAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAg                                                                                      <  1:105522/150‑1 (MQ=255)
                                                                 cccGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCAtgt                                                                               <  2:799166/151‑1 (MQ=255)
                                                                  ccGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCAtgtg                                                                              <  1:214523/151‑1 (MQ=255)
                                                                      aGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATc                                                                           >  1:496921/1‑150 (MQ=255)
                                                                         ggATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATg                                                                       <  1:673780/151‑1 (MQ=255)
                                                                           aTGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGc                                                                     <  1:620362/151‑1 (MQ=255)
                                                                            tGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGcc                                                                    >  2:314047/1‑151 (MQ=255)
                                                                               cgcgCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCt                                                                 <  2:50825/151‑1 (MQ=255)
                                                                                gcgcCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGGTTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTg                                                                >  1:731004/1‑151 (MQ=255)
                                                                                 cgcCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTgg                                                               <  2:221051/151‑1 (MQ=255)
                                                                                  gcCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTgg                                                               >  1:100056/1‑150 (MQ=255)
                                                                                   ccACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGAt                                                             <  1:805840/151‑1 (MQ=255)
                                                                                      cccGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGATCACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGa                                                           <  2:816445/150‑1 (MQ=255)
                                                                                       ccGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGaaa                                                         <  2:192461/151‑1 (MQ=255)
                                                                                              cTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCg                                                  >  1:509189/1‑151 (MQ=255)
                                                                                                gAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCg                                                  <  2:651539/149‑1 (MQ=255)
                                                                                                gAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGAc                                                <  1:848376/151‑1 (MQ=255)
                                                                                                    aaCGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAg                                            <  1:137441/151‑1 (MQ=255)
                                                                                                     aCGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGa                                           <  1:630681/151‑1 (MQ=255)
                                                                                                      cGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGat                                          <  2:258352/151‑1 (MQ=255)
                                                                                                       gTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGata                                         <  2:157054/151‑1 (MQ=255)
                                                                                                       gTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGat                                          >  1:257488/1‑150 (MQ=255)
                                                                                                             aTAGGCTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCt                                   >  2:449109/1‑151 (MQ=255)
                                                                                                                  cTTTTACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTcgcg                              >  1:712398/1‑151 (MQ=255)
                                                                                                                    tttACATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCgg                             >  1:570642/1‑150 (MQ=255)
                                                                                                                       aCATTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCa                         <  1:781005/151‑1 (MQ=255)
                                                                                                                         aTTCACATAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGc                       >  1:535104/1‑151 (MQ=255)
                                                                                                                            cacaTAAGGCGAACACTCATCTCCCCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCCTGAATATGCTCCCAGGTGGAGCCCATGTGATCAATGGCCCCTCGATGAAAGTGCGCGACTAAGATATCAGCTTCGCGGATCAGCtt                     <  2:300316/150‑1 (MQ=255)
                                                                                                                             acaTAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCtttt                   <  1:836975/151‑1 (MQ=255)
                                                                                                                             acaTAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCACGACTAAGATATCAGCTTCGCGGATCAGCtttt                   >  1:104157/1‑151 (MQ=255)
                                                                                                                               aTAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTcc                 >  2:497548/1‑151 (MQ=255)
                                                                                                                               aTAAGGCGAACACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTcc                 >  2:148292/1‑151 (MQ=255)
                                                                                                                                 aaGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCAt               >  1:842602/1‑151 (MQ=255)
                                                                                                                                       aaCACTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGACAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACctc         >  2:50755/1‑151 (MQ=255)
                                                                                                                                         cacTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCtt       <  2:156223/151‑1 (MQ=255)
                                                                                                                                           cTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTg     >  1:319790/1‑151 (MQ=255)
                                                                                                                                           cTCATCACACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTg     >  1:97509/1‑151 (MQ=255)
                                                                                                                                             catcaCACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGAAATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTg     <  1:333907/149‑1 (MQ=255)
                                                                                                                                              atcaCACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCt  >  2:33435/1‑151 (MQ=255)
                                                                                                                                              atcaCACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCt  <  2:520136/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTAGTGGATGCCGCGCCACCCGCTGCCTGAGCAACGTTTTCATAGGCTTTTACATTCACATAAGGCGAACACTCATCAAACCCTTTGATCGAACCAAAAATCAGACGTGGATTGATTTCTTGAATATGCTCCCAGGTGAAGCCCATGTGATCAATGGCCCCTGGATGAAAGTTCTCGACTAAGATATCAGCTTCGCGGATCAGCTTTTCCATTACCTCTTTGCCT  >  NC_000913/2492729‑2493021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: