Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,517,660	A→C	I58S (ATC→AGC)	yfeA ←	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,517,660	0	A	C	100.0%	212.8 / NA	54	I58S (ATC→AGC)	yfeA	putative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/29);  total (25/29)

TGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTT  >  NC_000913/2517513‑2517807                                                                                                                                                    |                                                                                                                                                    tGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTa                                                                                                                                                   >  2:818170/1‑150 (MQ=255)   ccAGCGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAAc                                                                                                                                                >  1:179231/1‑151 (MQ=255)    cAGCGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAAc                                                                                                                                                >  1:579821/1‑150 (MQ=255)       cGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACa                                                                                                                                               >  1:137505/1‑148 (MQ=255)       cGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACa                                                                                                                                               >  1:93976/1‑148 (MQ=255)          aaTATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGAtt                                                                                                                                          <  2:584283/150‑1 (MQ=255)           atatTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTg                                                                                                                                         >  2:519220/1‑150 (MQ=255)                  gcAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCAtt                                                                                                                                 <  1:192381/151‑1 (MQ=255)                     ggCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACg                                                                                                                              <  1:337534/151‑1 (MQ=255)                       cacacaACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGca                                                                                                                            <  1:281248/151‑1 (MQ=255)                       cacacaACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGc                                                                                                                             >  2:447592/1‑150 (MQ=255)                            aacaCGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTg                                                                                                                       <  1:587400/151‑1 (MQ=255)                              cacGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGAGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTg                                                                                                                       >  2:868980/1‑149 (MQ=255)                                    aaaCGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCAc                                                                                                               <  1:533877/151‑1 (MQ=255)                                          cTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGcc                                                                                                            >  1:322055/1‑148 (MQ=255)                                                   gAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGAtata                                                                                                 >  2:648903/1‑150 (MQ=255)                                                   gAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGAtata                                                                                                 >  1:557236/1‑150 (MQ=255)                                                       aTGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTgg                                                                                            >  1:517473/1‑151 (MQ=255)                                                          aCCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAAt                                                                                         >  2:251728/1‑151 (MQ=255)                                                           ccGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATg                                                                                        <  1:455141/151‑1 (MQ=255)                                                           ccGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATg                                                                                        >  2:372949/1‑151 (MQ=255)                                                            cGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATgg                                                                                       >  2:801034/1‑151 (MQ=255)                                                            cGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATg                                                                                        <  1:656681/150‑1 (MQ=255)                                                                    gTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATg                                                                                 <  2:322047/149‑1 (MQ=255)                                                                      aaaaaaGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCaa                                                                             <  2:905815/151‑1 (MQ=255)                                                                      aaaaaaGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCa                                                                              >  1:340593/1‑150 (MQ=255)                                                                       aaaaaGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAg                                                                            <  2:69638/151‑1 (MQ=255)                                                                       aaaaaGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAg                                                                            <  2:372715/151‑1 (MQ=255)                                                                           agagagATGAAAGGTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGc                                                                        >  2:859309/1‑151 (MQ=255)                                                                                gaTGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAAt                                                                   <  2:844524/151‑1 (MQ=255)                                                                                 aTGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAAt                                                                   >  2:577031/1‑150 (MQ=255)                                                                                   gAAAGTTGCACAGATGGGTGCAAAACATCCCGCATAAAACGGGAACGACGCCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATaa                                                                 <  1:589312/150‑1 (MQ=255)                                                                                          ccACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGAt                                                         <  1:921418/151‑1 (MQ=255)                                                                                              aGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCt                                                     >  2:277160/1‑151 (MQ=255)                                                                                              aGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCt                                                     <  1:5344/151‑1 (MQ=255)                                                                                              aGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCt                                                     >  2:547163/1‑151 (MQ=255)                                                                                                    gTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATa                                                >  2:846200/1‑150 (MQ=255)                                                                                                      gCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAg                                              <  1:447602/150‑1 (MQ=255)                                                                                                        aaaaCATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTAc                                           <  1:480970/151‑1 (MQ=255)                                                                                                         aaaCATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTAcc                                          >  1:685304/1‑151 (MQ=255)                                                                                                           aCATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCAc                                        <  2:627580/151‑1 (MQ=255)                                                                                                             aTCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACgg                                      <  1:125779/151‑1 (MQ=255)                                                                                                                  gCATAAAACGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGc                                 <  1:251733/151‑1 (MQ=255)                                                                                                                      aaaaCGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTa                              <  2:719126/150‑1 (MQ=255)                                                                                                                       aaaCGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTaaa                            <  1:856020/151‑1 (MQ=255)                                                                                                                        aaCGGGAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAAc                           <  1:764970/151‑1 (MQ=255)                                                                                                                             gAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAg                      <  2:550151/151‑1 (MQ=255)                                                                                                                             gAACGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAg                      <  1:231320/151‑1 (MQ=255)                                                                                                                               aCGACACCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAgtg                    <  1:77570/151‑1 (MQ=255)                                                                                                                                  acacCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGa                 <  1:72372/151‑1 (MQ=255)                                                                                                                                    acCGCGCCAGCCAAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAat               >  2:672143/1‑151 (MQ=255)                                                                                                                                          ccagccaAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTg         >  1:556848/1‑151 (MQ=255)                                                                                                                                          ccagccaAAGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTg         >  1:758040/1‑151 (MQ=255)                                                                                                                                                 aaGCTAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATAtttt  <  1:841251/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TGCCAGCGTAATATTGCGCAGGCACACAACACGACAAACGTCTGGCTACCGAGCATGACCGCAGTCTGTAAAAAAGAGAGATGAAAGTTCCACAGATTGGTGCAAAACATCCCGCATAAAACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGCTAGTGTGAATATCTTGATATTTT  >  NC_000913/2517513‑2517807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑