Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,487,1630CT45.8% 49.0 / 27.7 48intergenic (+200/‑72)hrpA/ydcFputative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base C (6/20);  new base T (22/0);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGGCGC  >  NC_000913/1487020‑1487310
                                                                                                                                                |                                                                                                                                                      
tattaATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGAAATACA‑AATTTCTCCttt                                                                                                                                                 >  2:251637/1‑151 (MQ=255)
    aaTTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAGAAAAAAAGAATTTCTTCCttttg                                                                                                                                               >  2:109266/1‑148 (MQ=255)
          aaaaCGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGc                                                                                                                                         <  1:561459/149‑1 (MQ=255)
            aaCGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCg                                                                                                                                     <  1:259038/151‑1 (MQ=255)
            aaCGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCg                                                                                                                                     <  1:8984/151‑1 (MQ=255)
               ggTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGAAAAAAA‑ATTTTCTCCttttcgaggggt                                                                                                                                        >  1:622456/1‑136 (MQ=255)
                    gggTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCAc                                                                                                                             <  2:91402/151‑1 (MQ=255)
                       tAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACAAACA‑AATTTCTCCTTTTTGCGGGGTCCGCCtctctc                                                                                                                             >  1:12434/1‑146 (MQ=255)
                       tAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGAAATAAG‑ATTTTCTCCTTTTTGAGGGCTCCGCCtctctc                                                                                                                             >  2:179976/1‑146 (MQ=255)
                        aaaaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACtatt                                                                                                                         <  2:852053/151‑1 (MQ=255)
                          aaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACtattat                                                                                                                       <  1:764251/151‑1 (MQ=255)
                               cAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCa                                                                                                                   <  2:138672/150‑1 (MQ=255)
                                   aaTTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAG‑AATTTCTCCCTTTTGAGGGGTCCCCCTCTTACTATTATTTTACGc                                                                                                                >  2:431003/1‑149 (MQ=255)
                                    aTTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGACAAACA‑ATTTTCTCCTTTTCTAGGGGTCCGCTTCTTACTATTAtttctccc                                                                                                                >  2:195818/1‑144 (MQ=255)
                                     ttCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAG‑AATTTCTCCTTTTAGAGGGGTCCCCCTCTCCCTATTTTTTTACGc                                                                                                                >  2:876352/1‑147 (MQ=255)
                                     ttCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGACAAAAA‑ATTTTCTCCTTTTTCAGTGGTTCCCTTCTCCCTATTTTTTCACGCa                                                                                                               >  1:66581/1‑148 (MQ=255)
                                     ttCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGCTAAAGACAAAAG‑AATTTCTCCTTTTAGAGAGCTCCCCTTCTCCCtattttttccccccgt                                                                                                             >  2:486681/1‑138 (MQ=255)
                                       cAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑t                                                                                                          <  2:631091/150‑1 (MQ=255)
                                            aaaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTaa                                                                                                    <  2:978421/151‑1 (MQ=255)
                                            aaaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGACAAACG‑ATTTTCTCCTTTTAGAGGGGTCCGCCTCTCCCTATTTTTTCACGCAGTA‑TTCtt                                                                                                      >  2:715130/1‑149 (MQ=255)
                                            aaaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAATAAG‑ATTTTCTCCCTTCATGGGGCTCCCCTTCTCCCTATTATCTCCCCCAggaatttt                                                                                                       >  2:546004/1‑142 (MQ=255)
                                             aaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAACA‑ATTTTCTCCTTTCTGAGTGGTCCGCTTTTCCCTATTATTTCACCCAGTA‑TTCtt                                                                                                      >  2:797724/1‑148 (MQ=255)
                                                        ctgctgCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGACAAAAG‑ATTTTCTCCTTTTCGAGAGCTCCGCTTCTCCCTATTATTTTCCGCCGTA‑Ttttttatgggaaaata                                                                                          >  2:180989/1‑137 (MQ=255)
                                                        ctgctgCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGAt                                                                                           <  1:365782/148‑1 (MQ=255)
                                                        ctgctgCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATa                                                                                          <  1:552062/149‑1 (MQ=255)
                                                            tgcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATAAA‑ATTTTCTCCTTTTTGAGTGCTGCCCTTTTCTCCTTTTTATCAAACAGGA‑TTTTTTAGGGAAAGAAAagga                                                                                      >  2:526300/1‑149 (MQ=255)
                                                               aaGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGACAAAAG‑ATTTTCTCCTTTCTGAGGGCTCCGCCTTTCCCTATTATCTCACGCAGCA‑TTCTTTAGGGGACGATAAGGA‑GGaa                                                                                 >  2:656666/1‑150 (MQ=255)
                                                                 gATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCa                                                                              <  2:102238/151‑1 (MQ=255)
                                                                     aataatGTTTATCTAAAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTTGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCa                                                                              <  1:959458/147‑1 (MQ=255)
                                                                       taatGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGAAAAAAC‑ATTTTCTCCTTTTAGAGGGCTCTGGTTTTCCCCATTATATCACCCCGGA‑TTTTTAAggggaaggtaa                                                                                         >  2:350914/1‑127 (MQ=255)
                                                                        aatGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATAAC‑ATTTTCTCCTTTTAAAGGGGTCCGCCTCTCACTATTTTCTCCCCCAGTATTTTTTAAGGGAA‑GATAAGGA‑GGAAACATGaa                                                                          >  2:575910/1‑148 (MQ=255)
                                                                         atGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAATACA‑AATTTTTCCTTTTTTTGTGGTCCCCCTTTCCCTATTTTTTTACGCCGGA‑TTTTTTAGGGGACGGTAAGGA‑GGAAACCTGAAA‑Att                                                                     >  1:174907/1‑151 (MQ=255)
                                                                               aTCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTAcccc                                                                <  2:344254/150‑1 (MQ=255)
                                                                                   aCAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGttt                                                            <  1:524435/150‑1 (MQ=255)
                                                                                    cAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTcc                                                          <  1:369341/151‑1 (MQ=255)
                                                                                         tttCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAC‑AATTCCTCCCTTCTGAGGGCCCCCCCTCTCACTATTATCTCACCCCGGA‑TTCTTTAAGGAACGAAAAAGA‑GGAAACAAGAAA‑ATTAcccccctttccacgc                                                     >  2:854672/1‑140 (MQ=255)
                                                                                               tAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTc                                                 <  2:688201/149‑1 (MQ=255)
                                                                                                aaaaGATATGTCAGGCTTGCGGAGTGGCGGTTTAGGAAAAAAA‑AATTTCTCCTTTTAGAGGGCTCCCCTTTTTTCTTTTTTTTTACCCCGGA‑TTTTTTAAGGAAAGATAAAGAGGGAACACTAAAA‑ATAACCCCGTTTccacccctt                                                   >  2:951577/1‑140 (MQ=255)
                                                                                                aaaaGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAACG‑ATTTTCTCCTTTTACAGGGGTCCCCTTCTCACTATTTTTTCCCGCAGGA‑TTCTTTAGGGGACGATAAAGA‑GGGAACATGAAC‑ATTAACCCCCTTTCGCCGCCTTTGcc                                              >  2:179179/1‑151 (MQ=255)
                                                                                                   aGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAG‑AATTTCTTCTTTTAGAGGGGTCCCCTTTTCACTTTTTTTTTTCCCCGTA‑TTTTTTAGGGGAAGATAAAGA‑GGAACCATGAACAATTCCCCCTTTTcctcgccttctc                                                >  1:25270/1‑137 (MQ=255)
                                                                                                      tatGTCAGGCTTGCGGAGTGGCGGTTTAGGACAAACA‑CCTTTTTCCTTTCATAGGGCTTCGCCTCTCCCCATTTTCTCTCCCCGTG‑TTTTTTAGGGAACGATAAAGA‑GGAACCATGAAC‑ACTTTCCCGTTTTCTGCGCTTTTGCCg                                             >  2:636325/1‑146 (MQ=255)
                                                                                                            aGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGa                                  <  2:672242/151‑1 (MQ=255)
                                                                                                                               tAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCgg                <  1:167825/150‑1 (MQ=255)
                                                                                                                                     cATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGtgg         >  1:336397/1‑151 (MQ=255)
                                                                                                                                        aCG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGtggctg      >  1:389015/1‑151 (MQ=255)
                                                                                                                                        aCG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGtggct       <  1:545993/150‑1 (MQ=255)
                                                                                                                                         cG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGtggctgg     >  2:919302/1‑151 (MQ=255)
                                                                                                                                              ttCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGgcgc  >  1:732626/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                      
TATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACG‑ATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTA‑TTCTTAAGGGAACGATAAGGA‑GGAACCATGAAC‑ATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGGCGC  >  NC_000913/1487020‑1487310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑