Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	43.9%	107.2 / 18.5	66	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (10/27);  new base G (29/0);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 1.49e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434733‑2435030                                                                                                                                                       |                                                                                                                                                      cAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGt                                                                                                                                                       <  2:44388/150‑1 (MQ=255) cAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTa                                                                                                                                                      <  2:693821/151‑1 (MQ=255)      ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATcc                                                                                                                                                  <  1:172045/150‑1 (MQ=255)           tttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAATCATAC‑CGGGCAGGGGAATCCAcc                                                                                                                                               >  2:628806/1‑148 (MQ=255)            ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGATTTGGATGAACCTAA‑CAGTCCGGGGAATCCa                                                                                                                                                 >  2:924956/1‑145 (MQ=255)              gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAATCATAC‑CGGTCAGGGGAATCCACCGCGAcc                                                                                                                                         >  2:415722/1‑151 (MQ=255)               ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCa                                                                                                                                        <  1:839640/151‑1 (MQ=255)                  aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGAt                                                                                                                                     <  1:107712/151‑1 (MQ=255)                   tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGGCAGGGGAATCCACCCCGACCAGAt                                                                                                                                     >  1:688253/1‑150 (MQ=255)                        tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAC‑CCGTCCGGGGAAACCACCGCGACCAGATACAGcc                                                                                                                               >  1:827751/1‑151 (MQ=255)                         gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGccc                                                                                                                              <  1:82328/151‑1 (MQ=255)                           aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAGCATAA‑CGGGCAGGGGAAACCACCCCGCCCAGGTAAAGccc                                                                                                                              >  1:688240/1‑149 (MQ=255)                           aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGGAATCCACCGCGACCAGATACAGCCCtt                                                                                                                            >  2:373257/1‑151 (MQ=255)                              cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTcc                                                                                                                          <  1:1013141/150‑1 (MQ=255)                              cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTcc                                                                                                                          <  2:137976/150‑1 (MQ=255)                                 cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAC‑CGGGCAGGGGAATCCACCGCGACCCGATAAAGCCCTTCCGCtt                                                                                                                      >  1:1050458/1‑151 (MQ=255)                                      ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTg                                                                                                                   <  2:978276/149‑1 (MQ=255)                                       tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTg                                                                                                                   <  1:269831/148‑1 (MQ=255)                                         aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAC‑CCGGCAGGGGAATCCACCCCGGCCAGATACAGCCCCTCCGCtttt                                                                                                                    >  1:821692/1‑145 (MQ=255)                                         aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGtt                                                                                                              <  1:721873/151‑1 (MQ=255)                                            cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCg                                                                                                                >  2:248600/1‑146 (MQ=255)                                                    aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGGCAGGGGAATCCACCGCGACCCGATACAGCCCCTCCGCTTTTGCCGTTGCTg‑ccccc                                                                                                    >  2:155083/1‑149 (MQ=255)                                                       ttGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGGAATCCACCCCGACCCGATACAGCCCTTCCCCTTTTTCCGTTGCTG‑CCGCCAGCg                                                                                                >  2:970905/1‑150 (MQ=255)                                                             aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTc                                                                                         <  2:245406/151‑1 (MQ=255)                                                               aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAC‑CGGGCAGGGGAATCCACCCGGGCCAGATAAAGCCCCTTCGCTTTTGGCGTTGCTG‑CCCCCCGCGtttttttc                                                                                        >  1:206362/1‑144 (MQ=255)                                                               aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAAATCAATA‑CAGTGCGGGGAATACACCGCCGCCAGACACAGCCCTTCCGCCTTTGCCGTTTCTGCCCCCCAGC‑TTCTGTCCt                                                                                       >  2:99783/1‑151 (MQ=255)                                                                 ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAC‑CGGGCAGGGGAAACCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTT‑CCCCCCGCGTTTTGTCCttt                                                                                     >  1:897020/1‑151 (MQ=255)                                                                 ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCttt                                                                                     <  2:224578/151‑1 (MQ=255)                                                                    gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTgcc                                                                                  <  2:407129/151‑1 (MQ=255)                                                                    gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTG‑CCGCCAGCGTTCTGTCCCTTgcc                                                                                  >  1:439880/1‑151 (MQ=255)                                                                    gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGACGATCATAC‑CCGTCAGGGGAATCCACCGCGACCAGATAAAGCCCCTCCCCCTTTTCCGCTGCTG‑CCCCCAACGTTCCGTCCTTTtcc                                                                                  >  2:117959/1‑151 (MQ=255)                                                                        tGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAC‑CCGGCAGGGGAAACCACCCCGGCCAGATACAGCCCCTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGGTCTGTTCTTTtccgcc                                                                               >  1:438118/1‑150 (MQ=255)                                                                         gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAC‑CCGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTtccgcc                                                                               >  2:824120/1‑149 (MQ=255)                                                                           tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTTGAAGATCATAA‑CCGTCAGGGGAATCCACCGCGGGCAGATACAGGCCTTTCCCTTTTGCCGTTGCTG‑CCGCCCGCGTTCTGTCCTTTtccgcc                                                                               >  1:326855/1‑147 (MQ=255)                                                                              gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAC‑CGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTTCCGCCCGCaa                                                                          >  1:674669/1‑149 (MQ=255)                                                                                 cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTg                                                                     <  1:411779/151‑1 (MQ=255)                                                                                 cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGGAATCCACCGCGACCAGAAACAGCCCCTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActct                                                                      >  2:509431/1‑150 (MQ=255)                                                                                  attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGGCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCCCTTTTGCCGTTGCTG‑CCCCCCGCGTTCTGTCCTTTTCCGCCCGCa                                                                           >  2:846489/1‑144 (MQ=255)                                                                                    tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCCTACTCGGT‑AGGGTAATCCATCGCGACCCGATAAAGCCCTTCCGTTTTTGACCGTGCTG‑CCTCCAGCGGTCTTTCCTTTGCCGCCAGCACCtcttct                                                                   >  2:525077/1‑150 (MQ=255)                                                                                     attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTAt                                                                 <  2:434691/151‑1 (MQ=255)                                                                                           gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGCAGATCATAC‑CCGGCAAGGGAATCCACCCCGACCAGGTACAGCCCCTCCGCTTTTGCCCCTTCTG‑CCGCCAGCGCTTTGTTCCTTTCCGCCCGCAAATCTGCTATcc                                                               >  2:828229/1‑147 (MQ=255)                                                                                           gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCCCTTTTGCCGTTGCTG‑CCCCCCGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATcc                                                               >  2:210630/1‑147 (MQ=255)                                                                                             tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctc                                                          <  1:517793/150‑1 (MQ=255)                                                                                                tCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCCGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTTTCCAGCTCTcc                                                       >  2:322438/1‑150 (MQ=255)                                                                                                 ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCg                                                      <  2:369227/150‑1 (MQ=255)                                                                                                 ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCg                                                      <  2:201828/150‑1 (MQ=255)                                                                                                  cGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGc                                                    <  1:190013/151‑1 (MQ=255)                                                                                                         aaTAGCGGGCCCATTGGCGGTTTTTGGAGATCATAC‑CGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTTCTG‑CCCCCAGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTATCCAGCTCTCCGGCTgggt                                               >  2:374393/1‑147 (MQ=255)                                                                                                         aaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgt                                             <  2:368505/151‑1 (MQ=255)                                                                                                            aGCGGGCCCATTGGCGGTTTTTGAAGATCATAC‑CGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCTTTTTCCGTTTCTG‑CCGCCAGCGTTCTGTCCTTTTCCCCCCGCAACTCTGCTATCCAGCTCTCCCGCTGGTTGTggg                                          >  1:669698/1‑151 (MQ=255)                                                                                                            aGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg                                           <  2:821709/150‑1 (MQ=255)                                                                                                             gCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGc                                         <  1:665000/151‑1 (MQ=255)                                                                                                                 gCCCATTGGCGGTTTTGGAAGATCATAC‑CGGGCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCTTTTGCCGGTGCTG‑CCGCCAGCGGTCTGGCCTTTGCCGCCCGCAACTCTGCTATCCAGCTCTCCCGCTGGTTGTGGGCAcc                                      >  1:41384/1‑150 (MQ=255)                                                                                                                    cATTGGCGGTTTTTGGAGATCAAAC‑CGGTCAGGGTAATCCACCGCGACCCGATACAGCCCCTCCGCTTTTTCCGTTGCTG‑CCGCCCGCGTTCTGTCCTTTGCCGCCCGCAACTCCGCTATCCAGCTCTCCGGCTGGTTTTGGGCAcc                                      >  1:584633/1‑147 (MQ=255)                                                                                                                        ggCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                               <  1:146541/150‑1 (MQ=255)                                                                                                                         gCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt                             >  2:399714/1‑151 (MQ=255)                                                                                                                            gTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                            >  1:290212/1‑149 (MQ=255)                                                                                                                                tGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                      >  1:106194/1‑151 (MQ=255)                                                                                                                                 ggAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                      <  2:307487/150‑1 (MQ=255)                                                                                                                                    aGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                  <  1:628794/151‑1 (MQ=255)                                                                                                                                       tCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACa                >  2:867879/1‑150 (MQ=255)                                                                                                                                        cATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACaa               <  2:603961/150‑1 (MQ=255)                                                                                                                                        cATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat              >  2:310776/1‑151 (MQ=255)                                                                                                                                            c‑cGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc          >  2:792733/1‑151 (MQ=255)                                                                                                                                                 tCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg       >  1:140096/1‑150 (MQ=255)                                                                                                                                                   aGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc    >  1:643430/1‑151 (MQ=255)                                                                                                                                                     ggTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat  <  1:209926/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      CAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAC‑CGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTG‑CCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434733‑2435030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑