New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3399175 =144 (1.140)3 (0.030) 3/280 25.5 2.2% coding (804/1104 nt) mreC cell wall structural complex MreBCD transmembrane component MreC
?NC_000913 = 3399171 134 (1.130)coding (808/1104 nt) mreC cell wall structural complex MreBCD transmembrane component MreC
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

TTGGTGATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3399332‑3399175
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgctgctgTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATCG  <  NC_000913/3399171‑3399024
                                                                                                                                                                                                                                                                                                                  
TTGGTGATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCT                                                                                                                                                             >  1:445554/1‑151
TTGGTGATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCT                                                                                                                                                             >  1:1503406/1‑151
 TGGTGATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCT                                                                                                                                                             >  1:42389/1‑150
    TGATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTG                                                                                                                                                         >  1:392100/1‑151
     GATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTTCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGC                                                                                                                                                        >  1:614777/1‑151
     GATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGC                                                                                                                                                        <  1:1293389/151‑1
     GATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGC                                                                                                                                                        >  1:1425561/1‑151
     GATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTG                                                                                                                                                         <  1:1967662/150‑1
     GATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTG                                                                                                                                                         <  2:1373432/150‑1
      ATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTG                                                                                                                                                         >  2:1609379/1‑149
                                                GCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGA                                                                                                             <  1:1274120/151‑1
                                                  TATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGG                                                                                                              >  2:1327518/1‑148
                                                                                                                                      CAACGTTTGCGTTATCTGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTAC                       >  2:1744466/1‑151
                                                                                                                                                     CTGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGG        >  1:1581284/1‑151
                                                                                                                                                      TGCTGCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGG       >  1:1965515/1‑151
                                                                                                                                                          GCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATC   >  2:1712003/1‑151
                                                                                                                                                          GCTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGAT    <  2:1430132/150‑1
                                                                                                                                                           CTGTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATCG  >  2:78376/1‑151
                                                                                                                                                                                                                                                                                                                  
TTGGTGATGTGCTGGTGACTTCCGGTCTGGGCGGTCGTTTCCCGGAAGGCTATCCGGTCGCGGTTGTCTCTTCCGTAAAACTCGATACCCAGCGCGCTTATACTGTGATTCAGGCGCGTCCGACTGCAGGGCTGCAACGTTTGCGTTATCTGCTGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3399332‑3399175
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgctgctgTGGGGGGCAGATCGTAACGGCGCTAACCCGATGACGCCGGAAGAGGTGCATCGTGTTGCTAATGAACGTCTGATGCAGATGATGCCGCAGGTATTGCCTTCGCCAGACGCGATGGGGCCAAAGTTACCTGAACCGGCAACGGGGATCG  <  NC_000913/3399171‑3399024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.