Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913213,3410AG41.9% 166.3 / 28.9 105G337G (GGA→GGGtilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (12/49);  new base G (44/0);  total (56/49)
Fisher's exact test for biased strand distribution p-value = 8.63e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCG  >  NC_000913/213192‑213491
                                                                                                                                                      |                                                                                                                                                         
gATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGaa                                                                                                                                                          <  1:82695/151‑1 (MQ=255)
     cTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAgtg                                                                                                                                                       >  2:412151/1‑149 (MQ=255)
           cTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGGACAGCtt                                                                                                                                               >  1:1933922/1‑151 (MQ=255)
           cTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTAGCTTCAACCGCTGGAATTACCGGCGGTGCAGGGGAGGGTaacggct                                                                                                                                               >  2:1548211/1‑145 (MQ=255)
            tGTTTACGCTTGGGCGCGTTTGAAATCCGACGCCATCAGTCGCACCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAACCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACa                                                                                                                                                   <  1:350178/146‑1 (MQ=255)
             gTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCtt                                                                                                                                               >  1:1168788/1‑149 (MQ=255)
                tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGGAGGGGACAGCTTaa                                                                                                                                             >  1:1424891/1‑148 (MQ=255)
                 aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAATGCg                                                                                                                                         >  1:216102/1‑151 (MQ=255)
                 aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCg                                                                                                                                         >  1:610589/1‑151 (MQ=255)
                 aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGc                                                                                                                                          <  1:1463515/150‑1 (MQ=255)
                  cGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGAGTACAGCCTaaatcgg                                                                                                                                        >  2:1711846/1‑146 (MQ=255)
                   gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATTCggg                                                                                                                                       >  2:1926108/1‑151 (MQ=255)
                   gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCggg                                                                                                                                       <  1:495582/151‑1 (MQ=255)
                       gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGc                                                                                                                                   <  2:280217/151‑1 (MQ=255)
                       gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGc                                                                                                                                   <  2:1490527/151‑1 (MQ=255)
                         gcgcGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAACTTAATGTgggggggg                                                                                                                                  >  1:1309656/1‑148 (MQ=255)
                             gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTAAAGCTTAATGCGGGGGGGGGTAtt                                                                                                                             >  2:1325862/1‑151 (MQ=255)
                                   aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTTATGCGGGAGGCGGTATTCGccc                                                                                                                        >  1:764919/1‑150 (MQ=255)
                                        gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGTGGAAAGCTTAATACGGGAGGCGGTATTTTCCCTCcgc                                                                                                                   >  2:528921/1‑150 (MQ=255)
                                         aCGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGCGGGGGGCGGTATTCGCCCTcccc                                                                                                                   >  1:1464422/1‑147 (MQ=255)
                                          cGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGTACAACTTAATGACGGAGGCGGTATTCTCCCTCcgcggg                                                                                                                >  2:1067564/1‑149 (MQ=255)
                                          cGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTg                                                                                                                <  1:1334177/151‑1 (MQ=255)
                                           gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGc                                                                                                               <  2:1695658/151‑1 (MQ=255)
                                             tATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGGAGTGTAAAGCTTAATGCGGGGGGGGGTATTTGCCCTCCCCGTg                                                                                                                >  1:900636/1‑148 (MQ=255)
                                              aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAg                                                                                                             <  2:1295614/150‑1 (MQ=255)
                                                 aGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCCTAATTCGGGAGGGGATATTTGCCCCCCCCCTGCAGAc                                                                                                           >  1:1689114/1‑149 (MQ=255)
                                                   tCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTTGGGAGAGTACAGCTTAATGCGGGAGGCGCTATTCTCCCTCCGCGTGCAg                                                                                                             >  1:185338/1‑145 (MQ=255)
                                                    cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGc                                                                                                      <  2:455986/151‑1 (MQ=255)
                                                     gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGGTTAATGCGGGGGGGGGTATTCGCCCTCCCCGGGCAGACGaa                                                                                                        >  1:1338324/1‑148 (MQ=255)
                                                     gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCg                                                                                                    <  2:1184502/151‑1 (MQ=255)
                                                     gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCg                                                                                                    <  2:1864872/151‑1 (MQ=255)
                                                       aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATGCGGGGGGGGATATTCGCCCTCCCCGGGCAGACGAAGCggg                                                                                                  >  2:1401873/1‑150 (MQ=255)
                                                       aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTTATTCGGGGGGCGGTATTCGCCCTCCCCGGGCAGACGAAGCgg                                                                                                   >  1:1820410/1‑150 (MQ=255)
                                                       aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAACTTAATTCGGGGGGGGGTATTTGCCCCCCCCCGGCAGACGAAGCgg                                                                                                   >  2:1929551/1‑150 (MQ=255)
                                                       aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGt                                                                                                  <  2:1651543/151‑1 (MQ=255)
                                                        aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTc                                                                                                 <  1:1908351/151‑1 (MQ=255)
                                                         cTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGCGGTATTTTCCCTCCGCGCGCAGACGAAACgg                                                                                                   >  1:708864/1‑148 (MQ=255)
                                                         cTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGCGGAATTCGCCCCCCGCGTGCAGACGAAACgg                                                                                                   >  1:960181/1‑148 (MQ=255)
                                                           gtggtgGATTAAATCCGTCGCCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc                                                                                              <  1:856504/151‑1 (MQ=255)
                                                           gtggcgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAAGGGATAACCTTAAGTGGGGAGGGGTTATTCCCCTCCCCTTGcgaacgaaaggggtta                                                                                                >  1:37505/1‑142 (MQ=255)
                                                             ggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgcgt                                                                                           <  1:1794669/151‑1 (MQ=255)
                                                              gtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgcgtg                                                                                          <  1:1641460/151‑1 (MQ=255)
                                                               tggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGTGGAAAGGCTAATACGGGAGGCGGTATTCGCCCTCCCCGTGCAGACGAAGCGGGCAgcg                                                                                            >  2:639540/1‑148 (MQ=255)
                                                                ggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgcgtgcg                                                                                        <  2:523629/151‑1 (MQ=255)
                                                                 gATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAATGCGGGGGGGGGTATTCGCCCTCCCCGCGGAGACGGAGCGGGCAgcgggcg                                                                                        >  1:214994/1‑150 (MQ=255)
                                                                     aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCa                                                                                   <  1:619001/151‑1 (MQ=255)
                                                                     aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCa                                                                                   <  2:1214829/151‑1 (MQ=255)
                                                                       aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAGCTTAATGCGGGGGGGGGTATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGCGGTTTaa                                                                                  >  2:1130615/1‑150 (MQ=255)
                                                                       aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCaaa                                                                                 <  2:1922617/151‑1 (MQ=255)
                                                                         ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAgc                                                                               <  2:192159/151‑1 (MQ=255)
                                                                         ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAgc                                                                               <  2:1859791/151‑1 (MQ=255)
                                                                         ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAgc                                                                               <  2:467904/151‑1 (MQ=255)
                                                                            tCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGAAAGCTTAAATCGGGGGGGGGTATTTGCCCCCCCCCGGGAGGCGAAGGGGGCAGCGGGCGGTTTaa                                                                                  >  1:1695356/1‑145 (MQ=255)
                                                                            tCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGGTTTTTGCGGGGGGGGAGATTTGCCCCCCGCGGGCCGACGAAACGGGCAGGGGGCGGTTTAAAGAGcc                                                                            >  1:555888/1‑151 (MQ=255)
                                                                             cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCa                                                                           <  2:21335/151‑1 (MQ=255)
                                                                             cACCGGGCAAAGCGAAAACATTGTGCCGCGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCa                                                                           <  2:282236/151‑1 (MQ=255)
                                                                              aCCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAAACGCTGGAATTACCGGCGGGGGGGGGGAGGGTACAGCCTAAATTGGGGGGGGGTATTTGCCCCCCCCGCGCCGGAGAAACGGGCAGCGGGGGGTTCaaaaccgc                                                                            >  1:610263/1‑144 (MQ=255)
                                                                                cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCGGGAGGCGATATTTGCCCTCCGCGGGCAGACGAAGCGGGCAGCGGGGGGTTCAAAACGCCAgg                                                                        >  2:790301/1‑150 (MQ=255)
                                                                                   gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCCTAATGCGGGGGGGGGGATTTTCCCCCCCCCCGCAGAAGAAGAGGGGCGCGGGCGGTTCCAAACGCCAGGagt                                                                     >  1:272219/1‑148 (MQ=255)
                                                                                    cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAAAGCGGGAGGCGGTATTTGCCCCCCCCCGGCAGACGAAGCGGGCAGCGTGCGGTTCCAAACGCCAGGAtt                                                                     >  2:1322709/1‑149 (MQ=255)
                                                                                     aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCCTAATTTGGGGGGGGGTATTCGCCCTCCGCGCGGAGACGAAGCGGGCAGCGGGCGTTTCAAAACGCCAgggatgc                                                                   >  1:1820020/1‑145 (MQ=255)
                                                                                     aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgct                                                                  <  2:764933/151‑1 (MQ=255)
                                                                                       aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTTATGCGGGGGGCGGTATTTCCCCCCCCCGTGCCGACGAAGCGGGCAGCGTGCGGTTCAAAACGCCAGGGTtgct                                                                  >  1:1587540/1‑149 (MQ=255)
                                                                                         cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTATGCGGGGGGGGGTATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGGGCGGTTTAAAACGcc                                                                            >  2:100520/1‑138 (MQ=255)
                                                                                          gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAGCTTTAAAGGGGGGGGGGTATTCCCCCTCCCCCTGCCGACGAAACGGGGAGCGGGGGGTTTCaaacgcccgggtt                                                                       >  1:1680350/1‑133 (MQ=255)
                                                                                           aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGATAATTGCCCCCCGCGTGCCGACGAAGCGGGCAGCGGGCGGTTCAAAACGCCCGGGTTGGTGCat                                                              >  1:82450/1‑149 (MQ=255)
                                                                                           aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCatat                                                            <  1:1416462/151‑1 (MQ=255)
                                                                                           aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCatat                                                            <  2:738975/151‑1 (MQ=255)
                                                                                                aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGAGTACAGCTTAATGCGGGAGGGGAGATTCGCCCTCCGCGTGGAGAAGAAGCGGGCAGCGTGCGTTTCAAAACGCCAGGATTGCTGGCTAtttttg                                                       >  2:1328318/1‑147 (MQ=255)
                                                                                                   gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc                                                    <  1:1125184/151‑1 (MQ=255)
                                                                                                     gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGt                                                  <  1:1095067/151‑1 (MQ=255)
                                                                                                       cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTaa                                                <  1:1066006/151‑1 (MQ=255)
                                                                                                        gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAATGCGGGGGGCGATATTCGCCCTCCGCGTGCAGACGAAACGGGCAGCGGGCGGTTCCAAACGCCAGGATTGCTGCATATTTTCggggggaa                                                >  2:810084/1‑145 (MQ=255)
                                                                                                        gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAc                                               <  1:1459421/151‑1 (MQ=255)
                                                                                                         tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGAAGTGTACAACTTCATGCGGGGGGCGGGATTCGGCCTCCCCGCGTGAACGAAACGGGTCAGGGGCGCTTCCAAAAGCCAGGATTGCTGCATATTGTCGGGCgcta                                                >  2:1640877/1‑146 (MQ=255)
                                                                                                           gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc                                            <  1:1873461/151‑1 (MQ=255)
                                                                                                            cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTAAGCGGGGGGGGGGATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGCGGGCGGTTCAAAACGCCCGGGTTGCTGCATATTTTCGGGCGGAAcgg                                             >  1:1575974/1‑149 (MQ=255)
                                                                                                                cGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCGGGAGGCGATATTCTCCCTCCCCGGGCAGACGAAGCGGGCAGCGTGGGGTTCAAAACGCCAGGATTGCTGCATATTGTCGGGGGGAAaggcgg                                          >  1:418551/1‑148 (MQ=255)
                                                                                                                cGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAAGGTGGAGCTTAATTTGGGGGGCGACATTCGCCCTCCCCGCGCAGACGAAGCGGGCCACGTGGGGTTTAAAGCGCCGGGAATTTTGGATAATGTTGGGCggtaaggggggt                                        >  1:1370130/1‑139 (MQ=255)
                                                                                                                cGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACg                                       <  2:219214/151‑1 (MQ=255)
                                                                                                                 gTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAACTTAATGCGGGGGGGGGTATTCGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGCGTTTTAAAGCGCCAGGGTTGCTGCCTATTGTCggggggaaacggggacg                                       >  2:1893056/1‑136 (MQ=255)
                                                                                                                  tGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGAGGCGGTATTTGCCCCCCGCCTGCAGACGAAGCGGGCAGCGGGCGTTTCAAAACGCCCGGGTTGCTGCATATTGTCGGGCGTAAAGGCGGACg                                       >  2:1487140/1‑149 (MQ=255)
                                                                                                                      ttCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGGTATTCGCCCTCCCCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTTCATATTTTCGGGCGTAACGGCGGACGTAAGCt                                 >  2:860357/1‑151 (MQ=255)
                                                                                                                       tCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGc                                  <  2:580390/149‑1 (MQ=255)
                                                                                                                       tCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCt                                 <  2:334841/150‑1 (MQ=255)
                                                                                                                        cAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTa                                <  1:1067546/150‑1 (MQ=255)
                                                                                                                          aCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAg                             <  2:1168809/151‑1 (MQ=255)
                                                                                                                           ccGCTGGAATTACCGGCGGGGCTGGGAAGAGTACAGCTTAATGCGGGGGGCGATATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGCGTTTTAAAGCGGCAGGGATTGTGCGTATTGTTGGGCGGAACGGCGGGCGGAAACTaaa                              >  2:759238/1‑149 (MQ=255)
                                                                                                                             gCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGaaa                          <  1:248621/151‑1 (MQ=255)
                                                                                                                             gCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGaaa                          <  1:1195824/151‑1 (MQ=255)
                                                                                                                                  aaTTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTg                     <  1:1677586/151‑1 (MQ=255)
                                                                                                                                      aCCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCaa                 <  2:865587/151‑1 (MQ=255)
                                                                                                                                       ccGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCa                  >  1:840001/1‑149 (MQ=255)
                                                                                                                                        cggcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTgg                    >  2:1011382/1‑146 (MQ=255)
                                                                                                                                          gcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc             <  1:647612/151‑1 (MQ=255)
                                                                                                                                              gggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg          >  1:71295/1‑151 (MQ=255)
                                                                                                                                                 cTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg       >  1:93101/1‑151 (MQ=255)
                                                                                                                                                   gggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGt      <  2:1021500/150‑1 (MQ=255)
                                                                                                                                                   gggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTg     >  1:684096/1‑151 (MQ=255)
                                                                                                                                                    ggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgc    <  1:1670609/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                         
GATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCG  >  NC_000913/213192‑213491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: