Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,133,3020TG40.6% 197.5 / 16.2 101H219P (CAC→CCC) yghSputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (6/54);  new base G (41/0);  total (47/54)
Fisher's exact test for biased strand distribution p-value = 3.17e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGGC  >  NC_000913/3133152‑3133451
                                                                                                                                                      |                                                                                                                                                     
gAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgt                                                                                                                                                       <  1:85685/151‑1 (MQ=255)
    gTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTc                                                                                                                                                    <  2:795155/150‑1 (MQ=255)
     tAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgggtt                                                                                                                                                    >  1:1337858/1‑148 (MQ=255)
             cTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCAccc                                                                                                                                            >  2:1020831/1‑149 (MQ=255)
             cTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATcc                                                                                                                                            >  2:1564705/1‑149 (MQ=255)
                cggcgCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTc                                                                                                                                       <  1:415376/151‑1 (MQ=255)
                  gcgcGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCaa                                                                                                                                     <  1:615280/151‑1 (MQ=255)
                   cgcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAg                                                                                                                                    <  1:205942/151‑1 (MQ=255)
                    gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAgg                                                                                                                                   <  2:924596/151‑1 (MQ=255)
                    gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGt                                                                                                                                         >  1:1649063/1‑145 (MQ=255)
                    gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCaa                                                                                                                                     >  2:459106/1‑149 (MQ=255)
                      ggTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAggttaaag                                                                                                                                   >  2:1304695/1‑142 (MQ=255)
                      ggTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGAt                                                                                                                                 <  2:458954/151‑1 (MQ=255)
                      ggTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCGTTCAAgg                                                                                                                                   >  2:1123557/1‑149 (MQ=255)
                       gTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCCGCCATCCCGCTCTAgggt                                                                                                                                 >  1:1012409/1‑148 (MQ=255)
                         tGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTTAAGGATCtt                                                                                                                              >  2:1956870/1‑151 (MQ=255)
                          gTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGGCGCCCATCCAGTTCAAGGAGCtt                                                                                                                              >  2:246284/1‑150 (MQ=255)
                           tACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGATCttt                                                                                                                             >  2:1543123/1‑150 (MQ=255)
                           tACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAAGaatttt                                                                                                                             >  2:1836243/1‑145 (MQ=255)
                             cTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTCAAGGAACtt                                                                                                                              >  2:1069922/1‑147 (MQ=255)
                               aTTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcg                                                                                                                          <  2:1669249/149‑1 (MQ=255)
                                 tAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAAGATCTTTTCGcc                                                                                                                        >  2:88664/1‑149 (MQ=255)
                                  aaCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCAtt                                                                                                                     <  1:1399007/151‑1 (MQ=255)
                                   aCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTg                                                                                                                    <  1:154802/151‑1 (MQ=255)
                                     agtttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGaa                                                                                                                  <  2:1691092/151‑1 (MQ=255)
                                      gtttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAAt                                                                                                                 <  2:1864950/151‑1 (MQ=255)
                                      gtttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTTAAGGATTCTTTCGCCCTTTaa                                                                                                                  >  2:1182500/1‑150 (MQ=255)
                                         tgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGt                                                                                                               <  2:1323742/150‑1 (MQ=255)
                                         tgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGCCCCTCCAGTTCCAGGATCTTTTCGCCCTTTAATGt                                                                                                               >  1:477189/1‑150 (MQ=255)
                                           agtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGCCCATCCCGTTCAAGGATCTTTTCGCCCTTGAATGTc                                                                                                              >  2:1879285/1‑149 (MQ=255)
                                            gtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAAGATCTTTGCGCCATTTAATGTCAg                                                                                                            >  2:721412/1‑150 (MQ=255)
                                            gtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATGTTTGCGCCATTTTATGttcgt                                                                                                           >  2:73516/1‑147 (MQ=255)
                                               tgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCCTCCCGATCAAGGGTCTTTTCGGCAATGAATGTCAGtt                                                                                                          >  1:1360525/1‑149 (MQ=255)
                                                 tAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGGTCAAAGATCTTTTCGCCCTTGTATGTCAGTTgggg                                                                                                      >  1:1394287/1‑151 (MQ=255)
                                                 tAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGCCCCTCCAGGTCAAGGATCTTTGCGGCCTTGAATGTCAGTTgcgg                                                                                                      >  2:1955096/1‑151 (MQ=255)
                                                  aGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTgcggc                                                                                                     <  1:211102/151‑1 (MQ=255)
                                                  aGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTgcgg                                                                                                      <  1:1122499/150‑1 (MQ=255)
                                                  aGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCTAGGAACTTTGCGCCATTGAATGTTCATTgcgg                                                                                                      >  2:1171642/1‑150 (MQ=255)
                                                           aTTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGGCCATCCAGTTTCAGGATCTTTTCGCCCTTGGATGTCAGGTGCGGCGGAACgg                                                                                              >  1:607976/1‑149 (MQ=255)
                                                              cGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAt                                                                                          <  1:1927222/150‑1 (MQ=255)
                                                                ttAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAttt                                                                                        <  1:21923/150‑1 (MQ=255)
                                                                 tAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAtttt                                                                                       <  1:978403/150‑1 (MQ=255)
                                                                  aaaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAGTTTAAGGATCTTTGCGCCATTGTATGTCAGTTGCGGCGGAAACGCGGtttttt                                                                                     >  1:1204492/1‑151 (MQ=255)
                                                                   aagcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAttttt                                                                                      <  1:1727151/146‑1 (MQ=255)
                                                                        tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGGGGGGTAACGGCGGTTTTTTTCttt                                                                                >  1:725866/1‑148 (MQ=255)
                                                                         ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAAGATCTTTGCGCCATTGAATGTCAGTTGGGGGGTAACGGCGATTTTTTTCttt                                                                                >  1:224029/1‑147 (MQ=255)
                                                                          cccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAAc                                                                             <  2:883223/151‑1 (MQ=255)
                                                                           ccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGATCTTTTCGCCATTGAATGTCAGTTGCGGCGGAACGGCGGTTTTTTTCTGtta                                                                              >  2:1378470/1‑147 (MQ=255)
                                                                              aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCt                                                                          <  1:1609627/150‑1 (MQ=255)
                                                                              aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTg                                                                         <  1:9948/151‑1 (MQ=255)
                                                                              aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCACCCCGTTCAAGGAGCTTTTCGCCCCTTTATTGCAGGTGTGGCGTGACGGCGGGTTTTTTCTTTTACg                                                                            >  1:1005628/1‑148 (MQ=255)
                                                                               tCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTg                                                                         <  1:388659/150‑1 (MQ=255)
                                                                                 cATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCa                                                                      <  1:1671223/151‑1 (MQ=255)
                                                                                    tttGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCCAGGATCTTTGCGCCATTGAATGTTAGTTGCGGCGTAACGGCGGTTTTTTTCTGTTAAGCTGcc                                                                       >  1:521136/1‑147 (MQ=255)
                                                                                     ttGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAGGATCTTTTCGCCCTTTAATGTCAGTTGCGGGGGAACGGCGGTTTTTTTCTTTAACGCTGCCAGt                                                                    >  2:1387874/1‑149 (MQ=255)
                                                                                        gTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtggtg                                                               <  1:1947740/151‑1 (MQ=255)
                                                                                         tGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGGAACGGCGGTTTTTTCCTGTAACGCCGCCAGTtggtgg                                                              >  2:1008277/1‑151 (MQ=255)
                                                                                            tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAAGTTAAGGATCTTTTCGGCATTTAATGTTAGTTGCGGCGGAAAGGCGGTTTTTTTCCGTAAAGCCGCCCACtggtgg                                                              >  1:1078890/1‑148 (MQ=255)
                                                                                            tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCCGATTAAGGATCTTTTGGCCATTGAATGTCAGTTGCGGGGGAACCGCGGTTTTTTTCTTTAAAACCGCCAATtgggggg                                                             >  1:1577292/1‑148 (MQ=255)
                                                                                             cAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCa                                                           <  1:1548865/150‑1 (MQ=255)
                                                                                             cAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAg                                                          <  1:1069571/151‑1 (MQ=255)
                                                                                              aGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAgg                                                         <  2:1102434/151‑1 (MQ=255)
                                                                                              aGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAgg                                                         <  2:1401097/151‑1 (MQ=255)
                                                                                                 aaaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGACCATCCCGTGCAAGGGACTTTGCGCCATTGAATAACCGTTGGGGCGTAAAGGCGGATTTTTTCTGTAACGCCGCCGGGtggtggggtgggtt                                                      >  2:1411903/1‑143 (MQ=255)
                                                                                                  aaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTa                                                     <  2:1421785/151‑1 (MQ=255)
                                                                                                  aaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTa                                                     <  1:1878489/151‑1 (MQ=255)
                                                                                                   aGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTGAAGGAACTTTTCGCCACTTAATGTTAGTTTGGGCGTAACGGAGATTTTTTTCTGTAAAGCTGACCGTTGGTGGTCAGGgtt                                                      >  2:1107163/1‑149 (MQ=255)
                                                                                                         gcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCaa                                              <  1:1604483/151‑1 (MQ=255)
                                                                                                           gTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCaaa                                             <  1:1731518/150‑1 (MQ=255)
                                                                                                             gAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAAcgc                                          <  1:1166525/151‑1 (MQ=255)
                                                                                                             gAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAAcg                                           <  2:1003220/150‑1 (MQ=255)
                                                                                                             gAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAAcg                                           <  1:216423/150‑1 (MQ=255)
                                                                                                             gAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAAcg                                           <  1:464748/150‑1 (MQ=255)
                                                                                                              aaTTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAAcgcg                                         <  1:1936706/151‑1 (MQ=255)
                                                                                                               aTTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCTCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAAcgcg                                         <  2:924725/150‑1 (MQ=255)
                                                                                                                ttGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCACCAGTTCAAGGATCTTTTGGCCATTGGATGGCAGTTGCGGCGTAAAGGGGGGTTTTTTCTGTTACGCTGGCCGGTGGGGGGCAGGGTTTCGCGCaaa                                             >  1:776172/1‑145 (MQ=255)
                                                                                                                ttGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTTCGGCGGAACGGCGATTTTTTTCTGTAACGCTGGCCGTTGGGGGGGCGGGTTACGCGCAAACGCgg                                        >  1:281995/1‑150 (MQ=255)
                                                                                                                 tGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTTAATGTCAGTTGCGGCGGAACGGCGGTTTTTTTCTGTAACGCTGCCCGGTGGGGGGCAGGGTTACGCGCCAACGCgg                                        >  1:463007/1‑149 (MQ=255)
                                                                                                                    gcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGt                                   <  2:1251456/151‑1 (MQ=255)
                                                                                                                    gcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGt                                   <  2:627794/151‑1 (MQ=255)
                                                                                                                     cgTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGAAAACGCGGTTTGtt                                  <  2:1558854/151‑1 (MQ=255)
                                                                                                                      gTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGCCCCAATCAGTTCCAGGGTCTTTGCGCCACTGGATGGCGGATGGGGGGGAACCGGGGCTTTTTTCTGTAACGCTTTCATTTCGTGGTCTGGCTTACCAGCAAAAGCGGgttgttt                                 >  1:1673282/1‑150 (MQ=255)
                                                                                                                        aGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtcat                               <  1:102752/151‑1 (MQ=255)
                                                                                                                           gACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtcatca                             <  2:873032/150‑1 (MQ=255)
                                                                                                                           gACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCaa                            <  1:1214410/151‑1 (MQ=255)
                                                                                                                            aCGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAGTTCAAGGATCTTTTCGCCATTGAATGTCAGTTGCGGCGGAACGGCGGTTTTTTCCTGTAAAGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGGTTGTTCATCaa                            >  2:745421/1‑150 (MQ=255)
                                                                                                                            aCGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAAt                           <  1:349855/151‑1 (MQ=255)
                                                                                                                             cGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAGGATCTTTTCGCCATTGAATGTTAGTTGCGGCGGAACGGCGGTTTTTTTCTGTTAAGCTGCCAGGTGGGGGGCCGGGTTACGCGCCAAAGCGGGTttttttt                               >  2:1026500/1‑143 (MQ=255)
                                                                                                                              gCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGc                         <  2:1971764/151‑1 (MQ=255)
                                                                                                                                 tGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCa                       <  1:321660/150‑1 (MQ=255)
                                                                                                                                  gCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCaa                      <  1:1911320/150‑1 (MQ=255)
                                                                                                                                     gAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACg                  <  1:552646/151‑1 (MQ=255)
                                                                                                                                      aaTTTCATCGGCGGGGGGGTGCCCCCCCACTTCAAAGATCTTTTCGCCATTGGATGTCAGTTGCGGCGTAACGGCGCTTTTTTTCCGTAACGCTGCCCGTTGGTGGGGCGGTTTTCGCGCAAACGCGGGTTGTTTATTAATGCCAacac                   >  1:915255/1‑149 (MQ=255)
                                                                                                                                          tCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATc              <  2:255878/150‑1 (MQ=255)
                                                                                                                                           cATCGGCGGGGGGTCGCCCATCCAGTTCAAAGATCTTTGCGCCATTGAATGTCAGTTTCGGCGTAACGGCGGTTTTTTCCTTTAACGCTGCCAGTTGGGGGGCAGGGTTTAGCGCAAACGCGGTTTGTTCATCAATGCCAAAAAGAATca             >  1:1571683/1‑150 (MQ=255)
                                                                                                                                            aTCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATcaac           <  2:565780/151‑1 (MQ=255)
                                                                                                                                              cggcggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATcaaca          <  1:404707/150‑1 (MQ=255)
                                                                                                                                                gcggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAAt        >  2:627724/1‑150 (MQ=255)
                                                                                                                                                  ggggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACg     >  1:1606249/1‑151 (MQ=255)
                                                                                                                                                     gtgtCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGGc  >  2:879711/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGGC  >  NC_000913/3133152‑3133451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: