Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913266,3490CA41.7% 225.4 / 53.3 115V69F (GTT→TTT) yafYlipoprotein, inner membrane; degP regulator; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base C (16/51);  new base A (48/0);  total (64/51)
Fisher's exact test for biased strand distribution p-value = 8.71e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGAT  >  NC_000913/266203‑266499
                                                                                                                                                  |                                                                                                                                                       
gTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGc                                                                                                                                                     <  1:1858128/151‑1 (MQ=255)
     tCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAAGTAAAAACTGCAAgg                                                                                                                                                 >  2:879540/1‑150 (MQ=255)
      cACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGt                                                                                                                                                <  1:1897077/150‑1 (MQ=255)
        cctcctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTgg                                                                                                                                              <  1:820035/150‑1 (MQ=255)
          tcctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACCGCAAgggggg                                                                                                                                             >  2:526966/1‑149 (MQ=255)
          tcctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACTGCAAgggggg                                                                                                                                             >  2:1625578/1‑149 (MQ=255)
           cctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGAc                                                                                                                                           <  1:1517611/150‑1 (MQ=255)
              ggTATGGCAGTTTGATGTTATCGGCAATACACGGCGCATTCATCAGTGGCTGGACGGTTTTCTCCAGCATAGCCACGAGATAGTAGTAACTGTCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGGCCgg                                                                                                                                        <  1:966635/150‑1 (MQ=255)
                 aTTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATa                                                                                                                                     <  2:847798/150‑1 (MQ=255)
                   tCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAag                                                                                                                                   <  2:1229832/150‑1 (MQ=255)
                   tCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAAGGGGGGCCGGATaa                                                                                                                                    >  1:940973/1‑149 (MQ=255)
                    ccAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAagt‑a                                                                                                                                <  1:213585/151‑1 (MQ=255)
                    ccAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGCACGTTAAACCTGAAAGGGGGGGCCGGGGAagt                                                                                                                                  >  2:879797/1‑150 (MQ=255)
                     cAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTGAGTAAAAACCTCAAGGTGGGAACGGGTaa                                                                                                                                    >  1:377425/1‑147 (MQ=255)
                     cAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAGGTAAAACACGCAAGGTGGGagccgcgaa                                                                                                                                    >  1:569235/1‑139 (MQ=255)
                         ttGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGCGGGAAAAACGCAAGGGGGGGCCgggaaa                                                                                                                                    >  1:99030/1‑139 (MQ=255)
                         ttGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGAGTAAAAACCGCAAAGGGGGGCCCGATaa                                                                                                                                    >  1:74075/1‑143 (MQ=255)
                           gATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAAc                                                                                                                          <  2:682884/150‑1 (MQ=255)
                            aTGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACCGCCAAGGGGGGCCGGAAAAAT‑AGTCaaa                                                                                                                          >  2:1080571/1‑148 (MQ=255)
                              gTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAACCTGCCAGGGGGGGCCGGGTAAGA‑AGTCAAACtg                                                                                                                       >  2:1342060/1‑150 (MQ=255)
                              gTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAAGTAAAAACTGCAAAGGGGGGCCGGGTAAAG‑AGTCAACCcgt                                                                                                                      >  2:937871/1‑151 (MQ=255)
                                tATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCtgtg                                                                                                                     <  1:1476924/150‑1 (MQ=255)
                                tATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACTGCAAGGGGGGACCGGGTAAAA‑AGTTAACCcgtgg                                                                                                                    >  2:1210192/1‑150 (MQ=255)
                                 aTCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACTGCAAGGGGGGGCCGGGTAAGT‑AGTCAAACcgtg                                                                                                                     >  2:1880181/1‑149 (MQ=255)
                                  tCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGAc                                                                                                                   <  1:1241291/150‑1 (MQ=255)
                                   cGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGAcc                                                                                                                  <  1:961841/150‑1 (MQ=255)
                                     gCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCAtt                                                                                                               <  1:1616129/151‑1 (MQ=255)
                                      cAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAGGGGGGGGCGGGTAAGT‑AGTCAAACCGTGACCAtt                                                                                                               >  1:899023/1‑150 (MQ=255)
                                                  cATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGAcatc                                                                                                  <  2:1194898/151‑1 (MQ=255)
                                                     tcatcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGAcatcat                                                                                                <  1:666140/150‑1 (MQ=255)
                                                     tcatcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACTGCCAGGGGGGGGCGGGGAAAA‑AGTGAACCTCTGACCACTTTTTCCTGAcatcat                                                                                                >  1:1618219/1‑150 (MQ=255)
                                                      catcaGTTGCGTGACGGTTTTTGCCTGCATATCCACGAGGTAGTAGTAACTGCCAACCTCGCTGCCATCTGGCGACTGGCTTGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCGGTGACCATTCGTCGCTGACATCATcc                                                                                              <  1:1318503/151‑1 (MQ=255)
                                                         caGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCtg                                                                                            <  1:1325748/150‑1 (MQ=255)
                                                          aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCtggt                                                                                          <  1:1081908/151‑1 (MQ=255)
                                                          aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCtggt                                                                                          <  1:400527/151‑1 (MQ=255)
                                                          aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAAGTAAAACCCTCAAGGGGGGGGCGGGAAAAG‑AATCAAACTTTGGGCATTCTTTTCTGGcatcaatct                                                                                             >  2:1641134/1‑144 (MQ=255)
                                                          aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAAGGAAAAACTGCAAGGGGGGGCCGGGTAAAG‑AGGCAAACCGTGAACATTCTTCGCTGACATCATCCtcg                                                                                           >  2:67780/1‑148 (MQ=18)
                                                           gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACCGCAAAGGGGGGCCGGATAAGA‑AGTCAACCTGTGGCCATTCTTCGCTGACATCATCCtggtg                                                                                         >  2:1223843/1‑151 (MQ=18)
                                                            tGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAAGGGGGGCCGGGTAAAG‑AGGCAAACTGTGACCATTCTTCGCTGACATCATCCtggggg                                                                                        >  2:1534884/1‑151 (MQ=18)
                                                              gCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCt                                                                                      <  2:870863/151‑1 (MQ=255)
                                                               cTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACCGCAAGGTGGGACCGGAGAAGT‑AGTCAACCCGTGACCATTCTTTGCTGACATCATCCTGGGGGcgg                                                                                     >  1:1652151/1‑149 (MQ=255)
                                                                tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACTGCAAGGTGGGGCCGGATAAGT‑AGTCAACCTGTGACCCTTCTTCCCTGACATCATCCtggtgg                                                                                        >  1:1268465/1‑147 (MQ=255)
                                                                 ggACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGa                                                                                    <  1:1544262/150‑1 (MQ=255)
                                                                 ggACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGAc                                                                                   <  2:1337922/151‑1 (MQ=255)
                                                                 ggACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACTGCAAGGGGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGGTGAc                                                                                   >  2:932507/1‑151 (MQ=255)
                                                                 ggACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGGAAAAACTGCCAGGTGGGGCCGGGTAAAG‑AGTCAACCTGTGACCATTCTTTGCTGACATCATCCTGGGGGCTGAc                                                                                   >  2:1801635/1‑151 (MQ=255)
                                                                   aCGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGGGGGGCCCGGTAAAT‑AGTCAACCCGTGACCCTTCTTCTCTGACATCATCCCGGGGGCTGGccc                                                                                 >  1:23112/1‑150 (MQ=255)
                                                                    cGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAAGTAAAAACTGCAAGGTGGGACCGGATAAGTAAGTC‑ACCAGTGACCATTCTTTGCTGACATCATCCtggtgggcgaac                                                                                  >  1:495288/1‑143 (MQ=255)
                                                                           ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCGCATCACt                                                                          <  2:1573336/150‑1 (MQ=255)
                                                                              cTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACTGAAAAACTGCAAGGGGGGGACCGAGAAAG‑AGTCAAACCGTGACCAATCTTTGCCGAACTCCACCTTGTGGGTGAcc                                                                                  >  2:731332/1‑139 (MQ=255)
                                                                              cTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCCAGGGGGGGACGGGTAAGT‑AGTTAACCTGTGAGCATTCTTCGCTGACATCATCCTCGTGGCTGCCCACATCACTgga                                                                       >  1:1396966/1‑148 (MQ=21)
                                                                                gCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACCGCAAGGGGGGGCCGGGTAAGG‑AGTCAAACTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACCGaaa                                                                      >  2:1866002/1‑149 (MQ=21)
                                                                                gCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACTGCAAGGGGGGGCCGGGTAAAT‑AGTCAAACTGTGACCATTCTTTCCTGACATCATCCTGGGGGGTGAACCCAACACTTaaa                                                                      >  1:1405639/1‑149 (MQ=255)
                                                                                    atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACCGCAAAGGGGGGCCGGAGAAGT‑AGTCAACCCGTGGACCTTCTTCGCTGACATTATCCTGGTGGGCGACCACATCACTGAAAagccg                                                                 >  1:656588/1‑148 (MQ=255)
                                                                                     tCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAAGGAAAAAATCCAAGGGGGGGCCGGATAAGT‑AAGCAACCCGGGACCATTCTTCGCTGAAAGCATCCTGGTGGGTGACCACATCACTTAAAAGCgg                                                                 >  1:424051/1‑149 (MQ=255)
                                                                                      ccACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTgg                                                              <  2:1681344/151‑1 (MQ=255)
                                                                                      ccACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAACCTGCAAGGGGGGGCCGGATAAGT‑AGTCAACCTGTGAACATTCTTCGCCGACATTATCCCGGTGGCTGACCACATCACTGAAACGCGgggg                                                              >  2:167760/1‑151 (MQ=255)
                                                                                        aCGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTgggg                                                            <  1:1598275/151‑1 (MQ=255)
                                                                                         cGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGt                                                           <  2:1944610/151‑1 (MQ=255)
                                                                                          gAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTg                                                          <  1:1782307/151‑1 (MQ=255)
                                                                                           aGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACCGCAAGGGGGGGCCGGGTAAAT‑AGTCAACCTGTGACCATTCTTTGCTGACATCATCCTGGTGGCTGACCACATCACCTAAACGCGgggggggg                                                          >  2:595753/1‑148 (MQ=255)
                                                                                             gtagtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAgg                                                       <  1:1648022/151‑1 (MQ=255)
                                                                                               agtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGt                                                      <  1:275322/150‑1 (MQ=255)
                                                                                               agtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTc                                                     <  1:723258/151‑1 (MQ=255)
                                                                                                gtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAGGGGGGACCGGGTAAAT‑AGTCAAACTGTGACCATTCTTCGCTGACATCATCCTGGGGGGTGACCACATCACTGAAAAGCGGTggggggggggcc                                                    >  2:1688589/1‑143 (MQ=18)
                                                                                                  agtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCtt                                                  <  1:331346/151‑1 (MQ=255)
                                                                                                  agtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAGGGGGGACCGGATAAGA‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGGGGGGGGAGGGCCtt                                                  >  2:918478/1‑151 (MQ=18)
                                                                                                   gtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTa                                                 <  2:679933/151‑1 (MQ=255)
                                                                                                   gtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTa                                                 <  1:885797/151‑1 (MQ=255)
                                                                                                    taACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGGAAAAACTGCAAGGGGGGGCCGGGAAAGG‑AGTCAACCTGTGGCCCTTCTTCGCTGACAACATCCTGGTGGCTGAACACAACACTGAAAAGCGGTGGGGGGGGGTCCtt                                                  >  2:1202981/1‑149 (MQ=255)
                                                                                                     aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTaaa                                               <  2:835614/151‑1 (MQ=255)
                                                                                                      aCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAAt                                              <  2:1756667/151‑1 (MQ=255)
                                                                                                      aCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACCGCCAGGGGGGACCGGATAAAG‑AGTCAACCTGTGACCATTTTTCGCTGACATCATCCTGGTGGGTGACCACATCACTGAAACGCGGGGGGGGGGGGGCCTTtaa                                               >  1:914944/1‑150 (MQ=255)
                                                                                                       cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGGAAAAACCGCAAGGGGGGGCCGGGTAAGT‑AATCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGGTGACCACATCACTTAAACGCGGGGGGGGGGGGGCCTTTAAtt                                             >  1:1439542/1‑151 (MQ=18)
                                                                                                       cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAACCTGCAAGGGGGGGGCGGATAAAT‑AGTCAAACTGTGACCCTTCTTCGCTGACATCATCCTGGTGGGTGACCACATCACTGAAAAGCGGGGGGGGGGGGGTCTTAAAtt                                             >  2:1461598/1‑151 (MQ=18)
                                                                                                        tGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGTCGTAAAAACCCCAAAGGGGGACCGGAGAAAA‑AGTCAACCTGTGACCAATTTTCGCTGACATCATCCCGGTGGGTGACCACAACACCGAAACGCGGTGGGGGGAGGGGCTTAAAttt                                            >  1:1080494/1‑151 (MQ=255)
                                                                                                         gCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACCGCAAGGGGGGGCCGGGTAAGT‑AGTCAAACTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGGGGGGGGGGGGCCTTAAAttt                                            >  2:950377/1‑150 (MQ=18)
                                                                                                           cATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACCGCAAGGGGGGGCCGGGTAAGT‑AGTCAACCTGTGACCCTTCTTCGCTGACATCATCCTGGGGGCTGACCACATCACCGAAAAGCGGGGGGGGGGGGGCCTTTAAttt                                            >  2:224959/1‑148 (MQ=18)
                                                                                                           cATCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAGGGGGGGCCGGGTAAAG‑AGTCAACCTCTGACCACTCTTCGCTGACATCCTCCCGGTGGGTGACCCCATCACTGAAACGCGGGGGGGGGGGGGCCTTAAAATTTCt                                         >  1:1788004/1‑151 (MQ=255)
                                                                                                            aTCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAAACTGCAAGGTGGGGCCGGGGAAAG‑AGGCAAACTGTGACCAATCTTCCCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGGGGGGGGGGGGCCTTTAAttt                                            >  1:556399/1‑147 (MQ=255)
                                                                                                            aTCCTCGCAGCCATCGGGCGACTGGCGGGGACGTTAAAACCGCAAGGTGGGGCCCGGGAAGG‑AGTCAACCCGTGGCCCTTCTTCGCTTACCTCATCCTGGTGGGTGACCACCACACCGAAACACGGGGGGGGGGGGGCCTTTAAttt                                            >  1:48065/1‑147 (MQ=255)
                                                                                                            aTCCTCGCAGCCATCGGGCGACTGGCGGGGACGTAAAAACTGCAAGGTGGGGCCGGATAAGG‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGGGGCTGACCCCATCACTGAAACGCGGGGGGGGGGGGTCCTTTAATTTACt                                         >  1:1753149/1‑150 (MQ=18)
                                                                                                                 cGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATgg                                    <  2:866374/150‑1 (MQ=255)
                                                                                                                    aGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCtt                                <  2:1107059/151‑1 (MQ=255)
                                                                                                                      ccATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCa                              <  1:1411695/151‑1 (MQ=255)
                                                                                                                       cATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAg                             <  1:320243/151‑1 (MQ=255)
                                                                                                                          cGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtc                          <  1:292131/151‑1 (MQ=255)
                                                                                                                          cGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtc                          <  1:451155/151‑1 (MQ=255)
                                                                                                                          cGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtc                          <  2:1934686/151‑1 (MQ=255)
                                                                                                                              cGACTGGCGGGTACGTAAAAACTACAAAGGGGGGCCCGAAAAAG‑AGTCAAACCGTGGCCATTCTTTCCTGACATCATCCCGGTGGCGGGCCCCAACACTGAAAAGCGGGGGGGGGGGGGCCTTTAATTTATTGTTGGCCTTTAgg                            >  1:67035/1‑145 (MQ=255)
                                                                                                                                   ggCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCt                 >  2:1843347/1‑151 (MQ=255)
                                                                                                                                          aCGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCg          <  2:1517079/151‑1 (MQ=255)
                                                                                                                                          aCGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCg          >  1:1864625/1‑151 (MQ=255)
                                                                                                                                           cGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGc         >  1:672946/1‑151 (MQ=255)
                                                                                                                                             tAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAt       >  2:1612618/1‑151 (MQ=255)
                                                                                                                                             tAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAt       >  2:93301/1‑151 (MQ=255)
                                                                                                                                              aaaaCCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAt       <  2:1052042/150‑1 (MQ=255)
                                                                                                                                              aaaaCCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATg      >  2:170638/1‑151 (MQ=255)
                                                                                                                                              aaaaCCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATg      <  2:1286326/151‑1 (MQ=255)
                                                                                                                                                  ccTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGAt  >  2:1366323/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                       
GTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGT‑AGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGAT  >  NC_000913/266203‑266499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑