Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC41.0% 228.5 / 26.6 118V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (60/9);  new base C (0/48);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 4.81e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427
                                                                                                                                                      |                                                                                                                                                       
cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑c                                                                                                                                                       >  2:100214/1‑151 (MQ=255)
  gACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑ccc                                                                                                                                                     >  1:751269/1‑151 (MQ=255)
    cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑ccccc                                                                                                                                                   >  1:178266/1‑151 (MQ=255)
    cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑ccccc                                                                                                                                                   <  1:1960536/151‑1 (MQ=255)
    cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑ccccc                                                                                                                                                   >  1:1951952/1‑151 (MQ=255)
      cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGc                                                                                                                                                 >  2:276434/1‑151 (MQ=255)
       aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGcc                                                                                                                                                <  2:852203/151‑1 (MQ=255)
       aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGcc                                                                                                                                                <  1:1666312/151‑1 (MQ=255)
       aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGcc                                                                                                                                                <  1:297945/151‑1 (MQ=255)
         agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCat                                                                                                                                              >  2:124709/1‑151 (MQ=255)
             tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTc                                                                                                                                          >  1:1513190/1‑151 (MQ=255)
              aaCACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTc                                                                                                                                          >  2:96724/1‑150 (MQ=255)
                cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACt                                                                                                                                       >  1:1412360/1‑151 (MQ=255)
                          ggttccccgggcgaaaaaTAAAGCAAATTTTTTGTCCGCCCTTCCCAACCAGTCCCCCCCCCAAACCATGCGGGGAGGGCCCCCGGGAAAAAAAAAATTCGGGAAGGAAAGATTGGCGGGT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                             <  1:875228/136‑1 (MQ=255)
                              gCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         >  2:1219315/1‑151 (MQ=255)
                               cccGGGCTGAAAATACAGCAAATTTGTTGCCACCCAGTCCCACCCAGTCCCCCCCCCGACCCATGCCGCGATGGTCGCGGGGAACAAAATAATACTGGAAGCGAAGTTTGCCGGGT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                             <  2:686146/146‑1 (MQ=255)
                               cccAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                        >  1:577239/1‑151 (MQ=255)
                                ccgggcgaaaaaaACAGCAAATTTGTTGCCAGCCAGTCCCAACAAGTCCCCCCCCCGAACCAGGACGCGAGGGTCGCCGGGAAAAAAATAATACTGGAAGCGAAGTTTGCGGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         <  1:1903994/140‑1 (MQ=255)
                                  aGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                                     >  2:1377909/1‑151 (MQ=255)
                                    gCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                   >  2:278917/1‑151 (MQ=255)
                                    gCTGAAAATACGGCGAATTTGTTGTCAGCCATTCCCACCAAGTCCCCCCCCCGAACCATGACGGGAGGTTCGCCGGAAACAAAATAATACGGGAAGCAAAGATTGCC‑GGA‑TTACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                   <  2:305162/151‑1 (MQ=255)
                                       gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                                >  1:418572/1‑151 (MQ=255)
                                         aaaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                              >  2:1892458/1‑151 (MQ=255)
                                             aCAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                          >  2:1756434/1‑151 (MQ=255)
                                             aCAGCAAAATTGTTGTCAGCCATTTCCACCCAGTCCCCCCCCCGACCCAGGCCGCGGGGGGCGCCGGGAACAAAATAATACTGGAAGCGAAGATTGCGGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                          <  2:1275752/151‑1 (MQ=255)
                                              cAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                          >  1:1549780/1‑150 (MQ=255)
                                               aGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  2:1594295/1‑151 (MQ=255)
                                               aGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  2:1812654/1‑151 (MQ=255)
                                                  gAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  2:788643/1‑148 (MQ=255)
                                                  aaaaTTGTTGCCACCCTTTTCCAACCAGTCCCCCCCCCGACCCATGACGCGAGGGCCGCGGGAAAAAAAAAAATACTGGAAGGAAAGATGGCGGGAA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagca                                                                                                         <  2:1723604/146‑1 (MQ=255)
                                                   aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                    >  1:393876/1‑151 (MQ=255)
                                                   aaaTTGTTGTCACCAAGTCCCAACCAGCCCCGCCCCCGAACCATGACGCGATGGTCGCGGGAAACAAAATAATACTGGAGGCAAAGTTTGCGGGAA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        <  1:1236139/147‑1 (MQ=255)
                                                   aaaTTGTTGCCAGCCATTCCCAACCAGTCCCGCCCCCAACCCATGCCGGGAGGGCCGCGGGAAAAAAAATAATACTGGAAGCGAAGTTTGCGGGGT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagca                                                                                                         <  1:666550/146‑1 (MQ=255)
                                                   aaaTTGTTGCCAGCCAGTCCCACCCAGTCCCGCCCCCGAACCAGGACGCGATGGTCGCCGGAAAAAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                    <  2:527433/151‑1 (MQ=255)
                                                      tttttgtCAGCCATTTCCAACCGGTCCCCCCCCCGAACCATGGCGCGATGGCCGCCGGAAACAAAATAATACTGGAAGCAAAGTTTGCGGGAT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                  <  1:1016025/147‑1 (MQ=255)
                                                      ttgttgTCACCCGCTCCCAACCAGTCCCCCCCCCGAACCATGCCGGGATGGCCGCCGGAAACAGAATAATATTGGAAGCAATTATTGCGGGTA‑TCC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGa                                                                                                     <  2:744723/147‑1 (MQ=255)
                                                        gttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                                >  2:563557/1‑150 (MQ=255)
                                                           gtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                                                                            >  2:578949/1‑151 (MQ=255)
                                                               gCCAGTCCCAACCAGCCCCCCCCCCAAACTAGGGCGCATTGTCCGCCGGTAAAAAAAAAATACTGAAAGCAAAGATTGCCGGTA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                           <  2:1214009/148‑1 (MQ=255)
                                                                ccattcccACCCAGCCCCCCCCCCGACCCATGACGGGAGGGTCGCGGGGAACAAAATAATACGGGAAGGGAAGATTGCGGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                         <  2:1782656/143‑1 (MQ=255)
                                                                ccAGTCCCAACCAGTACCCCCCCCAACCAAGGACGGGAGGGTCGCGGGGAAAAGAATAATACTGGAAGAGAAGATGGCCGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                         <  2:1662618/149‑1 (MQ=255)
                                                                    tcccAACCAGTCCCCCCCCCGAACCATGACGCGATGGTCGCCGGGAACAAAATAATACTGGAAGCGAAGATTGCCGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGa                                                                                    <  2:958174/148‑1 (MQ=255)
                                                                        aaCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                               >  2:886463/1‑151 (MQ=255)
                                                                        aaCCAGCCCCCCCCCCGACCCATGACGGGTTGGTCGCCGGAAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                                   <  2:908101/147‑1 (MQ=255)
                                                                         aCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                              >  1:105684/1‑151 (MQ=255)
                                                                          ccagccccccccccGACCCATGGCGCGATGGTCGCCGGAAACAAAAAAATACGGGAAGCGAAGTTTGCCGGGT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                               <  2:433758/140‑1 (MQ=255)
                                                                          ccagccccGCCCCCGACCCAGGCCGCGATGGCCGCCGGAAACAAAATAATTCTGGAAGCAAAGATTGCGGGAA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                              <  1:855113/144‑1 (MQ=255)
                                                                             gtcccccccccGACCCATGACGCGATGGTCGCCGAGAACAAAATAATACTGGAAGCGAAGTTTGCCGGGA‑TCC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                                                                             <  1:656481/142‑1 (MQ=255)
                                                                             gtcccGCCCCCGAACCATGACGCGGTGGTCGCGGGAAACAAAATAATACTGGAGGCGAAGATTGCCGGGT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                                <  2:962597/142‑1 (MQ=255)
                                                                              tACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                          >  2:1822763/1‑150 (MQ=255)
                                                                                 cccccccGACCCATGACGGGATGGTCGCCGAAAAAAGAATAATACTGGAGGCGAAGATTGCCGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                      <  2:750257/149‑1 (MQ=255)
                                                                                 cGCCCCCAAACCATGACGCGATGGTCGCGGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGTA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                      <  1:165738/151‑1 (MQ=255)
                                                                                     cccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                                  >  1:125560/1‑151 (MQ=255)
                                                                                      ccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                                  >  2:202051/1‑150 (MQ=255)
                                                                                        gAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                >  1:619544/1‑150 (MQ=255)
                                                                                           ccATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                             >  1:923477/1‑150 (MQ=255)
                                                                                             aTGACGCGATGGCCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                          <  2:1495940/151‑1 (MQ=255)
                                                                                              ggACGCGAGGGCCGCCGGAAAAAAAATAATACTGGAAGCGAAGATTGCCGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcgg                                                         <  1:612822/150‑1 (MQ=255)
                                                                                               gACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                        >  2:1621343/1‑151 (MQ=255)
                                                                                                aCGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                       >  1:153/1‑151 (MQ=255)
                                                                                                 cgcgATGGCCGCGGGAAACAAAAAAATACTGGAGGCGAAGATGGCCGGGT‑TCC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcgg                                                         <  2:1338833/148‑1 (MQ=255)
                                                                                                  gcgtggccccccGGAAAAAAAATAATA‑TTGAAACAAAAATTGGCGGTATTTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                           <  1:1136023/135‑1 (MQ=255)
                                                                                                       ggTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTACAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                                 >  2:168839/1‑150 (MQ=255)
                                                                                                       ggTCGCCGGAAACAAAATAATACTGAAAGCGAAGATTGCCGGGT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                                 <  1:1814079/150‑1 (MQ=255)
                                                                                                       ggTCCCCGGGAACAGAATAATACTGGAAGCGAGGATTGCCGGGT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCa                                                <  2:1548846/151‑1 (MQ=255)
                                                                                                          cGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACg                                              <  2:1128264/150‑1 (MQ=255)
                                                                                                              gggAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                          >  2:1965705/1‑150 (MQ=255)
                                                                                                              gggAAAAAAATAATCCGGGAAGCGAAGATTGCGGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                         <  2:1951988/151‑1 (MQ=255)
                                                                                                              ggaaaaaGAATAATACTGGAAGCGAAGATGGCGGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                           <  2:1898510/143‑1 (MQ=255)
                                                                                                               gaaaaaaaataatAGGGGAAACGAAGTTTGCCGGTT‑TTT‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                        <  1:253044/144‑1 (MQ=255)
                                                                                                                gAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                        >  1:1538910/1‑150 (MQ=255)
                                                                                                                 aaCAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                       >  1:1782861/1‑150 (MQ=255)
                                                                                                                 aaCAAAAAAATACTGGAAGCGAAGATTGCCGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                      <  1:770828/151‑1 (MQ=255)
                                                                                                                  aaaaaataatACGGGAAGCGAAGATTGCGGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                                     <  1:616202/147‑1 (MQ=255)
                                                                                                                  aCAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                      >  1:1710717/1‑150 (MQ=255)
                                                                                                                  aCAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                      >  1:1200577/1‑150 (MQ=255)
                                                                                                                   cAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                    >  2:1128055/1‑151 (MQ=255)
                                                                                                                      aataatACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  >  2:850283/1‑150 (MQ=255)
                                                                                                                      aataatACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  >  1:887173/1‑150 (MQ=255)
                                                                                                                         aatccggaaaGCAAAGTTTGCGGGGA‑TCC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                              <  1:174821/143‑1 (MQ=255)
                                                                                                                           taCTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                             >  2:1533844/1‑150 (MQ=255)
                                                                                                                           gtcTGGAAGCGAAAGTTCCCGGAT‑TCC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTGCCACAATGCGGCGTTGACCACGCTCAACAAATaca                                 <  1:1742804/144‑1 (MQ=255)
                                                                                                                            acgggAAGCGAAGTTGGCCGGAA‑TCC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                            <  1:1876390/147‑1 (MQ=255)
                                                                                                                              gggAAGCGAATTGTGCGGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                         <  1:1122914/150‑1 (MQ=255)
                                                                                                                               ggAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                        >  1:1206334/1‑151 (MQ=255)
                                                                                                                                 aggCGAGGATTGCCGGAA‑TCC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                        <  1:400489/147‑1 (MQ=255)
                                                                                                                                   gCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     >  2:482179/1‑150 (MQ=255)
                                                                                                                                    cGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCAc                   >  2:1425220/1‑151 (MQ=255)
                                                                                                                                      aaGATTGCGGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg                 <  2:1850854/151‑1 (MQ=255)
                                                                                                                                      aaGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg                 >  1:1820228/1‑151 (MQ=255)
                                                                                                                                       aGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc                >  1:1024862/1‑151 (MQ=255)
                                                                                                                                       aGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc                >  2:1415496/1‑151 (MQ=255)
                                                                                                                                        gtttGCCGGGT‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc                <  1:1338425/148‑1 (MQ=255)
                                                                                                                                          ttGCGGGGA‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa              <  2:663461/150‑1 (MQ=255)
                                                                                                                                               ggta‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         <  1:955332/147‑1 (MQ=255)
                                                                                                                                               gggA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         >  2:1038071/1‑150 (MQ=255)
                                                                                                                                               ggaa‑TTC‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaaa             <  1:1813952/143‑1 (MQ=255)
                                                                                                                                                 gA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat      >  1:413414/1‑151 (MQ=255)
                                                                                                                                                      a‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  2:1145630/1‑151 (MQ=255)
                                                                                                                                                      a‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  1:767293/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTA‑CCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑