Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,1760TC44.4% 113.8 / 60.3 100E304G (GAA→GGA) ftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (43/12);  new base C (0/44);  total (43/57)
Fisher's exact test for biased strand distribution p-value = 3.74e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGG  >  NC_000913/3601026‑3601326
                                                                                                                                                         |                                                                                                                                                       
tattatTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACtt                                                                                                                                                         >  2:477477/1‑151 (MQ=255)
 attattCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTc                                                                                                                                                        >  2:1151164/1‑151 (MQ=255)
 attattCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTc                                                                                                                                                        >  2:1402100/1‑151 (MQ=255)
   tattCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTcc                                                                                                                                                       <  1:1368048/150‑1 (MQ=255)
    attCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCg                                                                                                                                                      <  1:1921293/150‑1 (MQ=255)
       cAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AAc                                                                                                                                                 <  2:1852684/151‑1 (MQ=255)
          gCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AAccgc                                                                                                                                              <  2:10653/151‑1 (MQ=255)
           cGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AAccgcc                                                                                                                                             <  1:686185/151‑1 (MQ=255)
           cGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AAccgcc                                                                                                                                             <  2:1885666/151‑1 (MQ=255)
             tAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AAccgccg                                                                                                                                            >  2:57595/1‑150 (MQ=255)
              aaaGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGAt                                                                                                                                          >  2:1458830/1‑151 (MQ=255)
              aaaGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGAt                                                                                                                                          >  2:577431/1‑151 (MQ=255)
                      gTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCg                                                                                                                                  >  2:1454059/1‑151 (MQ=255)
                        aaaTGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCgc                                                                                                                                 >  2:345533/1‑150 (MQ=255)
                           tgttgtACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCgcagc                                                                                                                              >  1:233480/1‑150 (MQ=255)
                               gccccGTGGCAAGCCCCGCCCCCCCCCAGCCAATCATCGACCATACCAGCAGAAATAGCAGGCATTCATCGAATAATAAGCC‑ATTGATAACAACCTTCGTCCAAAAAA‑CC‑GGTGCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCa                                                                                                                          <  1:642689/147‑1 (MQ=255)
                                     tGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTc                                                                                                                   >  2:1555015/1‑151 (MQ=255)
                                     ggccagcccACGCCGCCACCCACCCAATCACGGAAAATACCAGAAAAAAGGGCGGGCTTCGATAAAATAATAACCC‑TTTGATATAAAGCTACGTCCAAAAAC‑CC‑GGTCCGACTACCGA‑AACCGCCGATGACAATCGCAGCACCAGAATTTc                                                                                                                   <  2:20897/144‑1 (MQ=255)
                                         aacccccgcccccccccAGCCAATCACCGAGCATCCCAGCAAAAATAGCAGGCTTTCATCAAATGATAACCC‑ATTGATATAAACCTTCGTCCCAAAAC‑CC‑TGTCCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGa                                                                                                                <  2:221493/136‑1 (MQ=255)
                                          acccccGCTCCCCCCCACCCAATCATCGAGCATACCAGCAGAAATAGCAGGCATCCATCGAATGATAAGCC‑ATTGATACAAAACTTCTTCCCAAAAC‑CC‑TGTCCCACTCCCGA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGa                                                                                                                <  2:1930774/144‑1 (MQ=255)
                                          aGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGAc                                                                                                               >  2:1183162/1‑150 (MQ=255)
                                            ccccgccgcCCCCAACCCAATCACCGAGAATACCAGCAGAAAAAGCAGACATTCAGAAAATGAAAAGCC‑ATTAATATCAACCTTCGTCCCAAAAC‑CC‑GGTCCCACTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGaaa                                                                                                              <  2:1766854/146‑3 (MQ=255)
                                            cccccctccccccaaGCCAATCACCGACCAACCAAGCACAAAAAGCAGGCATTCATCGAAAGAAAAGCC‑ATTAAAAAAAAACTTCTTCCCAAAAA‑CT‑TGTCCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACaaa                                                                                                            <  1:140839/137‑1 (MQ=255)
                                            ccACGCTGCCCCCCAGCCAATCATCGAGCATACCACCAAAAATAGCAGGCATTCATCGAATGATAAGCC‑ATTAATATCAACCTTCGTCCCGAAAC‑CC‑TGTGCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACaa                                                                                                             <  2:355841/150‑1 (MQ=255)
                                              aCGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAAt                                                                                                          >  1:1205604/1‑151 (MQ=255)
                                               cGCTCCCCCCCACCAAACCATAGAGCACACAAGCGAAAAAACCGGTCATTCAGAGAAT‑AAAAGCCAATTAATACAAACCTTCCTTCCAAAAC‑CG‑TGGCCCATTCCCCC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACa                                                                                                              <  2:341893/146‑1 (MQ=255)
                                                 cccccccccACCAAAACATCGAGCACCCCAACAAAAATGGCAGGCATTCATGGAATAATAACCC‑TTTGATAACAAAC‑TCCTTCCAAAAACCC‑TTTCCCACTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTa                                                                                                        <  1:1589373/144‑1 (MQ=255)
                                                  tGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAAt                                                                                                      >  2:118532/1‑151 (MQ=255)
                                                    ccccccacccaATCACCGAACACCCCAGCAAAAATAGCAGGCTTTCATAAAATAATACCCC‑ATTAATACAAACCTTCTTCCCAAAAC‑CC‑TTTCCCCCCCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACaaa                                                                                                            <  1:448132/140‑1 (MQ=255)
                                                    ccccccacccaACCACCGAGCATACCAGAACAAAAAGCAGGCATCCATCAAATAATAACCC‑ATTGATACAAACCTTCTTCCCAAAAC‑CC‑TGTCCCCCTCCCAC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGa                                                                                                    <  1:540824/148‑1 (MQ=255)
                                                    ccccccAGCCAATCATCGACCATCCCAGCAGAAATAGCAGGCTTTAATCGAATAATAACCC‑ATTAATAACAACCTTCGTCCCAAAAC‑CC‑TGTCCCACTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTaa                                                                                                       <  2:1152676/145‑1 (MQ=255)
                                                     cACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGac                                                                                                   >  2:877079/1‑151 (MQ=255)
                                                     cACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGac                                                                                                   >  2:910225/1‑151 (MQ=255)
                                                      cccaaGCAAATCATCGAACACACCAGCAGAAAAAGCAGTCATTCATCAAATAAAAACCA‑ATTAATATAAAACTTCGTCCCAAAAC‑CT‑TGTCCCACTCCCAC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGac                                                                                                   <  1:1928737/146‑1 (MQ=255)
                                                        ccAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGacaac                                                                                                >  2:120777/1‑151 (MQ=255)
                                                            ccAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATg                                                                                            >  1:801877/1‑151 (MQ=255)
                                                            ccAATCATCGAACACCCCAACAACAATAGCAGGTTTTCATAGAATGATAACCT‑TTTAATATAAATCTTC‑TTCCAAAAACCC‑TGTCCCCCTTCCGA‑ATCCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATg                                                                                            <  2:1150828/151‑1 (MQ=255)
                                                              aaTCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgcg                                                                                          >  1:1537564/1‑151 (MQ=255)
                                                                  acggacaaTACCAGCAAAAATAGCAGCTTTTCATCGAATAATAACCT‑ATTAACACAAACCTCCGTCCCAAAAC‑CC‑TGCCCCCCCCCCCA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCa                                                                                       <  2:448194/143‑1 (MQ=255)
                                                                  accgaaaaTACCACCAGAAATCGCGGCCTTTCATCAAATAAAAAGCA‑TTTGATATAAACCTCCGTCCCAAAAC‑CC‑TGTGCCACTCCCGA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAAt                                                                                             <  2:1020427/137‑1 (MQ=255)
                                                                   tcGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGcc                                                                                        >  2:934523/1‑148 (MQ=255)
                                                                   tcGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGcc                                                                                        >  2:6414/1‑148 (MQ=255)
                                                                    cGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGa                                                                                     >  1:394240/1‑150 (MQ=255)
                                                                    cGACCACACCAACAACAATACAAGACATTCATCAAAAAAAAAGCC‑ATTGATACAAAACTTCTTCCCAAAAA‑CC‑TTTCCCCCTCCCGA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAg                                                                                      <  1:207899/149‑1 (MQ=255)
                                                                     gAGCAACCCAGAAGAAAAAGCAGGAATTAATAAAATGATACGCT‑TTTTATATCAACCTTCGTCCCAAAAC‑CC‑TTTCCCACTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAg                                                                                      <  2:1936372/148‑1 (MQ=255)
                                                                      agcaacccaccaGAAAGAGCAGACATTCATCAAATGAAAACCT‑ATTAATAAAATACTTCTTTCAAAAAC‑CC‑TGCCCCCCTCACCA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAg                                                                                      <  1:1449499/141‑1 (MQ=255)
                                                                         aTACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATcc                                                                               >  1:950424/1‑151 (MQ=255)
                                                                             cagcacAAATGGCAGCCATTCATAAAATAAAAACCA‑ATTAATATCAACCTTCTTCCCAAAAC‑CC‑TGTCCCCCTCCCGA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCca                                                                             <  2:1513722/149‑1 (MQ=255)
                                                                                 agcaATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTg                                                                       >  2:1900742/1‑151 (MQ=255)
                                                                                 agaaaaaGCAGGCATTCATCAAATGAAAACCC‑ATTAATATAAACCTTCGTCCCAAAAC‑CC‑TGTCCCATTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATcc                                                                               <  2:938369/137‑1 (MQ=255)
                                                                                  gcaATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTgc                                                                      >  2:814633/1‑151 (MQ=255)
                                                                                   caATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTgcg                                                                     >  1:1671945/1‑151 (MQ=255)
                                                                                    aagaGCAGGCATTCATCAAATGATAACCC‑ATTAATATAAACCTTCGTCCAAAAAC‑CC‑TGTCCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTgc                                                                      <  1:312141/146‑1 (MQ=255)
                                                                                    aaaagcagcCATTCTCCAAATAATAAGCA‑TTTTATAACAACCTTC‑TTCCCAAAC‑CC‑CTGGCCACTTCCCCAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTgcgc                                                                    <  2:1747599/148‑1 (MQ=255)
                                                                                    aaTAGCAGGCTTTCACCAAATAAAAAGCT‑ATTAATATAAACCTTCGTCCCAAAAA‑CC‑TGTCCCACTCCCAC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCcag                                                                            <  2:710312/143‑1 (MQ=255)
                                                                                    aaTAGCAGGCATTCATCAAATGAAAAGCA‑TTTAATATCAACCTTCTTCCCAAAAC‑CC‑TGTCCCCCTCCCAC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTgc                                                                      <  2:1809623/149‑1 (MQ=255)
                                                                                      tAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGcc                                                                   >  1:1862959/1‑150 (MQ=255)
                                                                                        gCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGccacc                                                                >  2:419541/1‑151 (MQ=255)
                                                                                          tggCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCAt                                                              >  1:461194/2‑151 (MQ=255)
                                                                                           ggaaTTCATCAAATAATAAGCT‑ATTAATACAAACCTTCGTCCCAAAAC‑CC‑TGTCCCACTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGcca                                                                  <  1:1614212/143‑1 (MQ=255)
                                                                                           ggCATTCATCAAATAAAAAGCC‑TTTAATACAAAACTTCTTCCCAAAAC‑CC‑TGGCCCCCTCCCAC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATa                                                             <  2:1192079/151‑1 (MQ=255)
                                                                                             cATTCATCAATTGATAACCC‑TTTGATATAAAACTTCGTTCCAAAAC‑CC‑TGTGCCTCTTCCAA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACa                                                           <  2:1434752/151‑1 (MQ=255)
                                                                                                      aaTGATAAGCT‑ATTGATAACAAACTTCTTCCCGAAAC‑CC‑TGCCCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGccacca                                                               <  1:5860/138‑1 (MQ=255)
                                                                                                       aTGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGgcg                                                 >  2:714018/1‑151 (MQ=255)
                                                                                                           aaaaccaaTTAATATAAACCTTCGTCCCAAAAC‑CC‑TGTCCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGgcgc                                                <  1:534852/142‑1 (MQ=255)
                                                                                                            aaGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAgg                                             >  1:417795/1‑150 (MQ=255)
                                                                                                             acccctttaatacCAACCTTC‑TTCCAAAAA‑CCTTGTCCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAgg                                             <  2:1475155/141‑1 (MQ=255)
                                                                                                             aatcatttaatatCAATCTTC‑TTCCAAAAACCC‑TGTCCCACTCCCAA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGgcgc                                                <  2:453887/138‑1 (MQ=255)
                                                                                                             aGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGAt                                           >  2:605404/1‑151 (MQ=255)
                                                                                                              ccc‑ATTAATAAAAACCTTCG‑TCCAAAAA‑CCTTGCGCCACTCCCCA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGgc                                                  <  1:1736807/142‑1 (MQ=255)
                                                                                                                  aTTGATATAAACCTCCGTCCCGAAAC‑CC‑TGTCCCCCTTCCAC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGa                                         <  1:451702/149‑1 (MQ=255)
                                                                                                                   ttGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATc                                      >  1:109685/1‑151 (MQ=255)
                                                                                                                    taatacCAACCTTCGTCCAAAAAC‑CC‑TGCCCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATc                                      <  2:128214/148‑1 (MQ=255)
                                                                                                                    taataaaaatctccGTCCCAAAAC‑CC‑TGTCCCACTCCCGA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGa                                         <  1:1137650/134‑1 (MQ=255)
                                                                                                                      atatCAAACTTCTTCCCAAAAC‑CC‑TGTGCCTCTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATcc                                     <  2:759398/149‑1 (MQ=255)
                                                                                                                       tatCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATc                                  >  2:705427/1‑151 (MQ=255)
                                                                                                                         tCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTg                                >  2:776284/1‑151 (MQ=255)
                                                                                                                           aatcTTCGTCCCGAAAC‑CC‑TGTCCCCCTCCCGC‑AGCCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTg                                <  2:1422313/146‑1 (MQ=255)
                                                                                                                           aaccTTCGTCCAAAAAC‑CC‑TGCCCCCCTCCCCC‑ACCCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTc                              <  1:1581872/148‑1 (MQ=255)
                                                                                                                           aaaCTTC‑TTCCAAAAACCC‑TGTCCCACTCCCGA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCt                                 <  1:875601/148‑1 (MQ=255)
                                                                                                                            aaCTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCg                             >  1:366600/1‑151 (MQ=255)
                                                                                                                            aaCCTCCTTCCCAAAACCC‑TGCGCCACTCCCAA‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATc                                      <  1:983739/143‑1 (MQ=255)
                                                                                                                             aCTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGc                            >  2:1866197/1‑151 (MQ=255)
                                                                                                                              cTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACAATACAGGAACGGGCGCAGGATGAATCCATCTGTCGc                            >  1:1074592/1‑150 (MQ=255)
                                                                                                                                  gtcccaaaac‑CC‑TGTCCCATTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCa                        <  2:1097349/144‑1 (MQ=255)
                                                                                                                                   ttCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCaa                       >  2:1032967/1‑150 (MQ=255)
                                                                                                                                   tccaaaaaa‑CC‑GGCCCCCCTCCCAC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCAc                          <  1:1653155/142‑1 (MQ=255)
                                                                                                                                       gAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGt                  >  2:155608/1‑151 (MQ=255)
                                                                                                                                       aaaac‑CC‑TGCCCCCCTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGt                  <  1:1383919/150‑1 (MQ=255)
                                                                                                                                        aaac‑CC‑TGTCCCCCTCCCCC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAg                   <  1:1300426/149‑1 (MQ=255)
                                                                                                                                        aaac‑CC‑GGTCCCACTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATc                     <  2:767103/147‑1 (MQ=255)
                                                                                                                                        aaaa‑CC‑CGGCCCCCTTCCCCAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCa                    <  2:1083904/149‑1 (MQ=255)
                                                                                                                                         aaa‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGttt                >  1:1063908/1‑151 (MQ=255)
                                                                                                                                           a‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCt              >  1:1844316/1‑151 (MQ=255)
                                                                                                                                                tgtgCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGt            >  1:1499773/1‑150 (MQ=255)
                                                                                                                                                  tgCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACg         >  2:572143/1‑151 (MQ=255)
                                                                                                                                                    ccACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGtt       >  2:1771002/1‑151 (MQ=255)
                                                                                                                                                    ccACTCCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTa           <  2:642661/147‑1 (MQ=255)
                                                                                                                                                        ttCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATg   >  1:1937176/1‑151 (MQ=255)
                                                                                                                                                         tCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATgg  >  2:1621752/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                       
TATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCC‑ATTGATATCAAACTTCGTTCCGAAAA‑CC‑TGTGCCACTTCCGC‑AACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGG  >  NC_000913/3601026‑3601326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑