Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,8310TG41.2% 164.8 / 25.8 98N46T (AAC→ACC) hdfRflhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (16/41);  new base G (40/0);  total (57/41)
Fisher's exact test for biased strand distribution p-value = 2.03e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGC  >  NC_000913/3947682‑3947979
                                                                                                                                                      |                                                                                                                                                       
tGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGGAAAAGtt                                                                                                                                                        >  2:1563759/1‑151 (MQ=255)
 ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGtt                                                                                                                                                        <  2:540394/150‑1 (MQ=255)
 ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGtt                                                                                                                                                        <  1:1819255/150‑1 (MQ=255)
 ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTc                                                                                                                                                       <  2:849803/151‑1 (MQ=255)
     ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTcac                                                                                                                                                     >  2:1250344/1‑149 (MQ=255)
         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGGAAAGGGTCACA‑ccc                                                                                                                                                >  2:1275799/1‑149 (MQ=255)
          aGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAg                                                                                                                                              <  1:1133776/150‑1 (MQ=255)
             aaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGTTTACA‑CCCCGtt                                                                                                                                            >  1:1813013/1‑149 (MQ=255)
              aaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGAt                                                                                                                                         <  2:649736/151‑1 (MQ=255)
                       tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGGTCACA‑CCCAGTTGATTTTTCAGtt                                                                                                                                >  1:949122/1‑151 (MQ=255)
                       tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAGGGTCACA‑CCCAGTTGATTTTTCAGtt                                                                                                                                >  2:532693/1‑151 (MQ=255)
                        gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGtt                                                                                                                                <  2:933927/150‑1 (MQ=255)
                        gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTg                                                                                                                               <  1:825712/151‑1 (MQ=255)
                              aGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGAt                                                                                                                         <  2:1007487/151‑1 (MQ=255)
                               ggTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGAtt                                                                                                                        <  1:1128468/151‑1 (MQ=255)
                                  aTGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCg                                                                                                                      <  1:261401/150‑1 (MQ=255)
                                     cgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTCACA‑CCCAGGTGATTTTTCCATTGTCTGATTTGaaa                                                                                                                   >  2:644587/1‑150 (MQ=255)
                                      gcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTT‑ACC‑CCCACTTGATTTTCCAGttttttggtttggaa                                                                                                                   >  1:753172/1‑136 (MQ=255)
                                      gcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTT‑ACA‑CCCCGTTGATTTTTCAGTTTTCTTATTTGaaa                                                                                                                   >  1:645291/1‑149 (MQ=255)
                                        cACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTT‑ACA‑CCCAGGTGGTTTTTCAGTTGTCTGATTTGaaaagtc                                                                                                               >  1:652699/1‑147 (MQ=255)
                                           ctcctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAGGGTCACA‑CCCAGTTGATTTTTCCGTTGTCTGGTTTGAAAGCTc                                                                                                               >  2:704190/1‑148 (MQ=255)
                                            tcctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGGAAAAGTTCACA‑ACCCGGTGATTTTTCAGTTTTCTGCTTCTAAAGCCCAc                                                                                                             >  1:392654/1‑149 (MQ=255)
                                             cctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGAATCGAAAGCTCAct                                                                                                            <  1:402868/149‑1 (MQ=255)
                                              ctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCCGTTGATTTTTCCGTTGTTTGATTTGAAAgacc                                                                                                               >  2:1966360/1‑142 (MQ=255)
                                              ctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTCAAA‑ACCAGTTGGTTTTCCAGTTGTCTGATTTGAAAACTCAccg                                                                                                           >  2:143344/1‑147 (MQ=255)
                                                tACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCActgct                                                                                                         <  2:1743324/149‑1 (MQ=255)
                                                tACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGGAAAAGGTCACA‑CCCCCTTGATTTTTCAGTTGTCTGATTCGGAAGCTc                                                                                                               >  1:994144/1‑143 (MQ=255)
                                                 aCGGGCGGCCTGCCAGGTGGTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTCACA‑CCCCGTTGCTTTTTCCGTTGTCTGATTTGAAAACTCACCGCTGAc                                                                                                      >  2:740374/1‑151 (MQ=255)
                                                 aCGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGAc                                                                                                      <  2:443803/151‑1 (MQ=255)
                                                      cggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTCACA‑CCCAGTTGATTTTTCAGTTGTCTGATTCGAAAGCTCACTGCTGACTgg                                                                                                   >  2:817954/1‑149 (MQ=255)
                                                       ggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAGGTTCACA‑CCCAGTTGATTTTTCAGTTGTCTGTTTCTAAAACTCACCGCTGACTggg                                                                                                  >  2:1512644/1‑149 (MQ=255)
                                                         ccTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGGTCACA‑CCCAGTTGATTTTTCCGTTGTTTGATTCGAAAACTCACTGCTGACTggg                                                                                                  >  2:804160/1‑147 (MQ=255)
                                                            gCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTCACA‑CCCCGGTGGTTTTTCAGTTGTCTGGTTCGAAAACTCACTGCTGACTGGG‑GCAGa                                                                                            >  2:699083/1‑149 (MQ=255)
                                                             ccAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGGTCACA‑CCCCGTTGATTTTTCAGTTGTTTGATTTGAAAACTCACTGCTGACTGGG‑TCAGATa                                                                                          >  2:122811/1‑150 (MQ=255)
                                                             ccAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAGGGTTACA‑CCCCGTTGATTTTTCAGTTGTCTGATTCGAAAACTCACTGCTGACTGGG‑TCAg                                                                                             >  2:907326/1‑147 (MQ=255)
                                                              cAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATaga                                                                                        <  2:478954/151‑1 (MQ=255)
                                                                ggTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGc                                                                                      <  1:87065/151‑1 (MQ=255)
                                                                 gTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGGAAAAGGTAACA‑ACCCCGTGAGTTTCTAGTTTTTTGATTTTAAAACTTACTGCTGACTTGG‑TCTGATaa                                                                                         >  1:1091615/1‑146 (MQ=255)
                                                                   gCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTT‑ACA‑CCCAGTTGGTTTTTCCGTTTTCTGATTTGAAAGCTCACTGCTGACTTGG‑TCAGAAAGAGCg                                                                                     >  2:665284/1‑149 (MQ=255)
                                                                   gCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTTACA‑CCCAGTTTATTTTTCAGTTGTTTGATTTGAAAGCCTACTGCTGACTAGG‑TCAGATAGAGCg                                                                                     >  2:1143706/1‑149 (MQ=255)
                                                                     tCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTTACA‑CCCCGGTGTTTTTTTATTTTTCTGATTTGGAAAAAAACTGCTGACTGGG‑TGAGATAGAGCg                                                                                     >  1:1045293/1‑147 (MQ=255)
                                                                        tGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGTGGGCAAGGCTTACA‑CCCAGATGTTTTTTTCGGTGGTTGTTTAGAAAGCTCTCTCCTGTCCGTG‑GCAGATAGAGCGA‑tttacc                                                                             >  2:1925663/1‑147 (MQ=255)
                                                                         gAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGcc                                                                            <  1:930903/151‑1 (MQ=255)
                                                                          aGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTCACA‑CCCAGTTGATTTTTCAGTTGTCTGATTCGAAAACTCACTGCTGACTGGG‑GCAGATAGAGGGA‑TTCAGcc                                                                            >  2:1820413/1‑150 (MQ=255)
                                                                             gTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTc                                                                        <  1:94538/151‑1 (MQ=255)
                                                                             gTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTCACA‑CCCAATTGGTTTTTCAGTTGTCTGATTCTAAAGCTCACCGCCGACTGGG‑TCAGATAGAGCGA‑TTTAGCCGccc                                                                        >  2:298515/1‑149 (MQ=255)
                                                                               ttCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCg                                                                       <  2:1578994/150‑1 (MQ=255)
                                                                                tCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGa                                                                      <  1:758494/150‑1 (MQ=255)
                                                                                  tGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGGTCACA‑CCCAGTTGATTTTTCAGTTGTTTTATTCGGAAGCTCCCCGCTGACTGGG‑GCAGATAGAGCGA‑TTTAGCCCCTCGAcc                                                                    >  1:560449/1‑150 (MQ=255)
                                                                                   gCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGAcc                                                                    <  1:238819/149‑1 (MQ=255)
                                                                                   gCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCa                                                                   <  2:1661298/150‑1 (MQ=255)
                                                                                    cATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCaaa                                                                 <  1:592819/151‑1 (MQ=255)
                                                                                      tAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAGGGTCACA‑CCCCGTTGATTTTTCAGTTGGCTGGTTTGAAAGCTCACTGCTGAACGGG‑TCAGAGAGAGCGG‑TTCAGCCGCCCGACCcaa                                                                 >  2:770400/1‑149 (MQ=255)
                                                                                       aaGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTTACACCCCAGTGGTTTTTCC‑GTTTTTTGGTTTGGAAGCTCACTGCTGACTGGG‑GCAGATAAAGCGA‑TTCAGCCGCCCCACCaaa                                                                 >  1:1022362/1‑148 (MQ=255)
                                                                                       aaGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTCACA‑CCCCGTTGGTTTTTCAGTTTTCTGATTTGAAAACTCACTGCTGACTGGG‑GCCGAAAGAGCGA‑ATCAACCCCTCGACCAAAATg                                                              >  2:681881/1‑151 (MQ=255)
                                                                                         ggCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGAc                                                            <  2:1045493/151‑1 (MQ=255)
                                                                                                gtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTTTCA‑CCCAATTGATTTTTCCGTTTTCTGATTCGAAAGATTACCGCCGGCCGGG‑TCAGGTAGAGCGA‑ATCAGCCGCCCGCGCAAAGGGAAGCGCTCg                                                     >  1:1479600/1‑151 (MQ=255)
                                                                                                 tttttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGTAAAAGGTCACA‑CCCCGTTGATTTTTCCGTTGTCTGATTTGAAAGCTCACTGCTGACTGGG‑TGAGATAGAGCGA‑TTCAGCCGCCCGACCAAAGAGAagc                                                          >  1:309153/1‑145 (MQ=255)
                                                                                                 tttttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTTACA‑CCCAGTTGATTTTTCAGTTGTCTGATTTGAAAAATCACTTCTGAATGGG‑GCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGAAGCGTTCgg                                                    >  2:1899673/1‑151 (MQ=255)
                                                                                                    ttCACCAGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTCACA‑CCCCGTTGATTTTTCAGTTTTTTTGTTTGGAAACTCACTCCTGACTGGG‑GGAGAAAAAGGGAGTTCGCCCGC‑CCACCAAAAGGACGCGGTCgggt                                                  >  1:268899/1‑148 (MQ=255)
                                                                                                     tCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTa                                                 <  2:725422/150‑1 (MQ=255)
                                                                                                      cACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAAc                                               <  2:1899950/151‑1 (MQ=255)
                                                                                                       aCCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTCACA‑ACCCGATGATTTTCCAGTTGTCTTATTTGAAAACTCACTTCTGACTGGG‑GCAGATAGAGAGA‑TTTAACCGCCCGAACAAAATGACGCGTTCgg                                                    >  2:1681921/1‑145 (MQ=255)
                                                                                                          ggCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTCACA‑CCCCGTTGATTTTTCAGTTGTCTGGTTCGAAAACTCACTGCTGAATGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAATGACGCGTTCGGCTAACTTcc                                           >  2:1514523/1‑151 (MQ=255)
                                                                                                          ggCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGCGGAAAGGGTCTAA‑CCCCATTTAGTTTTCCGTTGTCTGATTTCAAAAATCACTGCTGGCTCGG‑GTAGATAAAGGGA‑TTTAGCCCCTCCACCAAAATGACGGGTTCGGCTaaacttc                                           >  1:494852/1‑146 (MQ=255)
                                                                                                           gCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCa                                          <  1:171064/151‑1 (MQ=255)
                                                                                                            cAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGGAAAGGTTCACA‑ACCACTTGGTTTTTCCGTTTTTTTATTTGTAAGCTCACTGCTGACTGGGTTCAG‑TATAGCGG‑TTTAGCCGCTCGACCAAAGTGACGCGTTCTGCTTACTTcccg                                         >  1:855788/1‑149 (MQ=255)
                                                                                                            cAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGGGGAAAAGGTCACACCCCAGTGGTTTTTCC‑GTTTTTTTGTTCTGAAACTCCCTGCTGACTGGG‑GGCGATAGAGGGA‑TTTAGCCGCTCGGCCCAAAAGAAGCGTTCGGCTAACtt                                             >  1:1033868/1‑147 (MQ=255)
                                                                                                              gCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGGAAAAGGTCACA‑CCCCGTTGATTTTTCCGTTGTCTGGTTCGAAAGCTCCCTGCTGACTGGG‑GCAGAAAGAGCGA‑TTTAGCCGCCCCACCCAAATGACGCGTTCGGCTAACTTCCAgg                                        >  2:350284/1‑150 (MQ=255)
                                                                                                              gCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAAGGTTACA‑CCCAAATGGTTTTTCAGTTTTTTGGTTTGAAAACTCACTGCTGACTGGG‑GCAGATAGAGGGA‑TTCAAGCGCCCCGCCCAAAAGACGCGGTCGGCTAACTTCCCgg                                        >  1:1176632/1‑150 (MQ=255)
                                                                                                               cGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGaa                                      <  2:819592/151‑1 (MQ=255)
                                                                                                               cGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGaa                                      <  2:1501908/151‑1 (MQ=255)
                                                                                                                ggTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGaaa                                     <  2:385725/151‑1 (MQ=255)
                                                                                                                ggTTAAACGGATATTGTTTCTGTGGC‑GGGGGGAAAAGGTCACA‑CCCCGTTGATTTTTCCGTTGTTTGATTCGAAAACTCACTGCTTACTGGG‑TCAGATAGAGCGG‑TTCAGCCGCTCGACCAAAAGGACGCGGTCGGCTAACTTCCAGGaa                                      >  2:1205402/1‑150 (MQ=255)
                                                                                                                    aaaCGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGttt                                 <  1:1714889/151‑1 (MQ=255)
                                                                                                                      aCGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGttttt                               <  1:995472/151‑1 (MQ=255)
                                                                                                                       cGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTa                              <  1:1177197/151‑1 (MQ=255)
                                                                                                                       cGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTa                              <  2:1383278/151‑1 (MQ=255)
                                                                                                                       cGGATATTGTTTCTGTGGC‑GGGGGAAAAAGGTCACA‑CCCAGGTGATTTTCCAGTTTTCTGTTTTGAAAGCCCACTGCTGACTGGG‑GCAGATAGAGCGA‑TTCAGCCGCCCGACCACAGTGACGCGTTCGGCTAACTTCCAGGGAAGttttt                               >  1:218885/1‑150 (MQ=255)
                                                                                                                        ggATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTaa                             <  1:1666867/151‑1 (MQ=255)
                                                                                                                        ggATATTGTTTCTGTGGC‑GGGTGAAAAAGGTCACA‑CCCCGTTGATTTTTCAGTTGTCTGATTTGAAAACTCACTGCTGACTGGG‑GCAGATAGAGGGA‑TTCAGCCGCTCGACCAAAGAGACGCGGTCGGCTAACTTCCAGGAAAGgtttt                               >  2:579983/1‑149 (MQ=255)
                                                                                                                        ggATATTGTTTCTGTGGC‑GGGGGAAAAAGGTACCA‑CCCCGGTGATTTTTCCGTTGTCTGATTCGAAAGATCACTGCTGACTGGG‑GCCGGAAGAGCGA‑TTCAGACGCTCGCCCCAAGTGACGCGGTCGGCTAACTTCCCGGAAAGgtttt                               >  1:1608949/1‑149 (MQ=255)
                                                                                                                            atTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACaa                          <  2:1681286/150‑1 (MQ=255)
                                                                                                                               gTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTc                       <  2:599991/150‑1 (MQ=255)
                                                                                                                                 ttCTGTGGC‑GGGGGAAAAGGGTCACA‑CCCAGGTGATTTTTCCGTTTTCTGATTCGAAAGCTCACTGCTGACTGGG‑GCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGGTCGGCTAACTTCCCGGAAAGGTTTTAACAATTcc                      >  1:545162/1‑149 (MQ=255)
                                                                                                                                   cTGTGGC‑GGGTGAAAAAGTTTAAA‑CCCAGATGATTTTCCACATTTCTGCTTCGAAAGCGCACTGCTTACTGGG‑TCAGAAAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTCACTTCCAGGAAAGGTTTTTACAATTCCGTAt                  >  1:1782785/1‑151 (MQ=255)
                                                                                                                                   cTGTGGC‑GGGTGAAAAAGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGATCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTa                   >  1:512977/1‑150 (MQ=255)
                                                                                                                                     gtgGC‑GGGTGGAAAGGGTTACA‑CCCAGTTGATTTTTCCGTTGTCTGATTCGAAAACTCACTGCTGACTGGG‑GCAGATAGAGCGA‑TTCAGCCGCCCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTTACAATTCCGTAt                  >  1:539294/1‑149 (MQ=255)
                                                                                                                                     gtgGC‑GGGTGGAAAGGGTCACC‑CCCACTTGGTTTTTCAGGTGTTTGGTTTGAAAGCTCACTGCTGACTTGG‑GCAGATAGAGCGA‑TTCAGCCGCCCCGCCAAAGTGACGCGTTTGGCTCACTTCCAGGAAAGGTTTTTACAATTTCg                     >  1:1332449/1‑146 (MQ=255)
                                                                                                                                       ggC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcac              <  1:58476/151‑1 (MQ=255)
                                                                                                                                       ggC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcac              >  2:1095287/1‑151 (MQ=255)
                                                                                                                                        gC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTACCTTCCAGGAAGGTTTTTACCAATTCGGAATc                 >  1:489815/1‑147 (MQ=255)
                                                                                                                                        gC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcac              >  1:1838002/1‑150 (MQ=255)
                                                                                                                                         c‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcac              >  1:532210/1‑149 (MQ=255)
                                                                                                                                         c‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcac              >  2:1848939/1‑149 (MQ=255)
                                                                                                                                               gAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGctc       <  1:1563728/151‑1 (MQ=255)
                                                                                                                                                aaaaGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGctc       <  2:563695/150‑1 (MQ=255)
                                                                                                                                                  aaGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGAATCCACAGTGCTCTcc    <  2:36789/151‑1 (MQ=255)
                                                                                                                                                   aGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCg   <  1:1241129/151‑1 (MQ=255)
                                                                                                                                                    ggTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCGCGACCAAAGTGACGCGTTCGGATAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGc  <  1:1602450/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC‑GGGTGAAAAGGTTCACA‑CCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGG‑TCAGATAGAGCGA‑TTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGC  >  NC_000913/3947682‑3947979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑