Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,915,297 G→A R80C (CGT→TGT)  proQ ← RNA chaperone, putative ProP translation regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,915,2970GA99.2% 507.5 / ‑4.4 131R80C (CGT→TGT) proQRNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (64/66);  total (65/66)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 132 total aligned reads displayed.

CCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGC  >  NC_000913/1915149‑1915442
                                                                                                                                                    |                                                                                                                                                 
ccAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACAcacg                                                                                                                                                 <  1:940686/151‑1 (MQ=255)
     agTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTGCATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTgcg                                                                                                                                            >  1:1637629/1‑151 (MQ=255)
         gCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCg                                                                                                                                         <  1:1574068/150‑1 (MQ=255)
           ggCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGt                                                                                                                                      >  1:1456015/1‑151 (MQ=255)
            gCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGtt                                                                                                                                     <  2:491335/151‑1 (MQ=255)
             cAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGttt                                                                                                                                    >  2:460298/1‑151 (MQ=255)
             cAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGttt                                                                                                                                    >  2:1790614/1‑151 (MQ=255)
              aGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTa                                                                                                                                   <  1:787605/151‑1 (MQ=255)
                cTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAac                                                                                                                                 >  1:347927/1‑151 (MQ=255)
                 ttCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAac                                                                                                                                 >  2:1171664/1‑150 (MQ=255)
                   cgcgTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACAcc                                                                                                                              <  1:1171952/151‑1 (MQ=255)
                    gcgTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCg                                                                                                                             >  2:1958448/1‑151 (MQ=255)
                    gcgTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCg                                                                                                                             <  2:1805021/151‑1 (MQ=255)
                     cgTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGt                                                                                                                            <  1:692393/151‑1 (MQ=255)
                     cgTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGt                                                                                                                            >  1:1181117/1‑151 (MQ=255)
                     cgTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGt                                                                                                                            >  1:922181/1‑151 (MQ=255)
                      gTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGt                                                                                                                            >  1:255765/1‑150 (MQ=255)
                       ttttttCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTa                                                                                                                           >  2:797428/1‑150 (MQ=255)
                         ttttCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTaaa                                                                                                                         <  2:1900426/150‑1 (MQ=255)
                          tttCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTaa                                                                                                                          >  2:405775/1‑148 (MQ=255)
                          tttCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTaa                                                                                                                          >  2:750823/1‑148 (MQ=255)
                                ttGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGc                                                                                                                 >  1:1065728/1‑151 (MQ=255)
                                 tGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACg                                                                                                                  <  1:1831316/149‑1 (MQ=255)
                                     gTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCt                                                                                                            <  1:453018/151‑1 (MQ=255)
                                     gTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCt                                                                                                            <  2:484376/151‑1 (MQ=255)
                                      ttCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTc                                                                                                           >  1:1295124/1‑151 (MQ=255)
                                       tCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTc                                                                                                           <  1:1243853/150‑1 (MQ=255)
                                        cAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCg                                                                                                          >  2:1202232/1‑150 (MQ=255)
                                           cACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGa                                                                                                         >  1:1012368/1‑148 (MQ=255)
                                           cACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAgt                                                                                                      >  2:1554666/1‑151 (MQ=255)
                                           cACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAg                                                                                                       >  2:1492851/1‑150 (MQ=255)
                                             cGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAgtgt                                                                                                    >  1:1742090/1‑151 (MQ=255)
                                             cGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAgtgt                                                                                                    <  1:1368790/151‑1 (MQ=255)
                                              gCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAgtgt                                                                                                    >  1:1195584/1‑150 (MQ=255)
                                                 gtgCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTag                                                                                                  >  2:1563588/1‑149 (MQ=255)
                                                       gAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTa                                                                                           <  1:1255914/150‑1 (MQ=255)
                                                         acacGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAg                                                                                        <  1:24323/151‑1 (MQ=255)
                                                             gcgcTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCgg                                                                                     >  1:1610721/1‑150 (MQ=255)
                                                             gcgcTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGa                                                                                    <  2:1868782/151‑1 (MQ=255)
                                                             gcgcTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGa                                                                                    <  2:956071/151‑1 (MQ=255)
                                                              cgcTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGAt                                                                                   <  2:1658515/151‑1 (MQ=255)
                                                              cgcTTTCGCTTCTTCAAGCTGCTTGCAAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGAt                                                                                   <  1:1491135/151‑1 (MQ=255)
                                                                  ttCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGc                                                                                >  1:1852275/1‑150 (MQ=255)
                                                                  ttCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCg                                                                                 <  1:1966075/149‑1 (MQ=255)
                                                                  ttCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGc                                                                                <  1:1097117/150‑1 (MQ=255)
                                                                   tCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCa                                                                               >  2:1211635/1‑150 (MQ=255)
                                                                   tCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCGCGAAGTGTAGAGACGTAAAGCGGATCGCa                                                                               >  1:206647/1‑150 (MQ=255)
                                                                     gCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAAtt                                                                            >  1:814345/1‑151 (MQ=255)
                                                                       ttcttcAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGc                                                                          >  1:697939/1‑151 (MQ=255)
                                                                         cttcAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGt                                                                        >  1:422002/1‑151 (MQ=255)
                                                                         cttcAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGt                                                                        >  1:785796/1‑151 (MQ=255)
                                                                              aGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGc                                                                   <  1:1464309/151‑1 (MQ=255)
                                                                                   ctTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAgg                                                              >  1:1391546/1‑151 (MQ=255)
                                                                                    ttGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGt                                                             >  2:1448513/1‑151 (MQ=255)
                                                                                    ttGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGt                                                             >  2:1438226/1‑151 (MQ=255)
                                                                                     tGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAgg                                                              <  1:1732524/149‑1 (MQ=255)
                                                                                     tGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAgg                                                              <  1:1017954/149‑1 (MQ=255)
                                                                                       cGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTc                                                           >  1:1064434/1‑150 (MQ=255)
                                                                                       cGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCa                                                          <  1:1163896/151‑1 (MQ=255)
                                                                                        gAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCAt                                                         <  2:1720338/151‑1 (MQ=255)
                                                                                         aGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCAtt                                                        >  2:1148153/1‑151 (MQ=255)
                                                                                          gCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCAttt                                                       <  2:365104/151‑1 (MQ=255)
                                                                                              gCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTcccc                                                   >  1:1598817/1‑151 (MQ=255)
                                                                                                tctACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTcccc                                                   >  1:411773/1‑149 (MQ=255)
                                                                                                 ctACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGc                                                >  1:1650900/1‑151 (MQ=255)
                                                                                                  tACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGCAGGGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGc                                                >  2:639987/1‑150 (MQ=255)
                                                                                                  tACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGc                                                >  2:1760176/1‑150 (MQ=255)
                                                                                                   aCATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCa                                               >  2:1024806/1‑150 (MQ=255)
                                                                                                     aTGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAaca                                            <  1:1154948/151‑1 (MQ=255)
                                                                                                     aTGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAaca                                            <  1:154540/151‑1 (MQ=255)
                                                                                                     aTGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAac                                             <  2:1574946/150‑1 (MQ=255)
                                                                                                       gTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAacac                                           <  2:133164/150‑1 (MQ=255)
                                                                                                       gTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAacac                                           <  2:938867/150‑1 (MQ=255)
                                                                                                          gCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATc                                       <  2:107075/151‑1 (MQ=255)
                                                                                                            tCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCg                                      >  1:1252245/1‑150 (MQ=255)
                                                                                                              gTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGAcc                                   >  1:760130/1‑151 (MQ=255)
                                                                                                                ccAGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCa                                  <  2:239475/150‑1 (MQ=255)
                                                                                                                  aGCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAAt                               <  2:1384972/151‑1 (MQ=255)
                                                                                                                   gCTCACCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATc                              >  1:1702754/1‑151 (MQ=255)
                                                                                                                       aCCGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGa                          <  2:894905/151‑1 (MQ=255)
                                                                                                                        ccGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGaa                         <  1:844188/151‑1 (MQ=255)
                                                                                                                        ccGCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGaa                         <  1:648575/151‑1 (MQ=255)
                                                                                                                          gCATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGaaa                        >  2:399409/1‑150 (MQ=255)
                                                                                                                           cATGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGaaaa                       >  2:1316584/1‑150 (MQ=255)
                                                                                                                            aTGGGTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAAt                     <  1:247440/151‑1 (MQ=255)
                                                                                                                              gggTTGCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATAc                   <  1:238314/151‑1 (MQ=255)
                                                                                                                                   gCCGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGAt               <  1:1594964/150‑1 (MQ=255)
                                                                                                                                    ccGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGAttt             <  2:1674043/151‑1 (MQ=255)
                                                                                                                                    ccGTCAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGAttt             <  1:1392667/151‑1 (MQ=255)
                                                                                                                                        cAAGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCa          <  1:1856480/150‑1 (MQ=255)
                                                                                                                                          aGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCg       <  1:1312264/151‑1 (MQ=255)
                                                                                                                                          aGATCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCg       <  1:176391/151‑1 (MQ=255)
                                                                                                                                            aTCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGa     <  1:740989/151‑1 (MQ=255)
                                                                                                                                             tCGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGAc    >  1:1289027/1‑151 (MQ=255)
                                                                                                                                              cGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGAcg   <  2:1844274/151‑1 (MQ=255)
                                                                                                                                              cGACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGAcg   <  2:1690424/151‑1 (MQ=255)
                                                                                                                                               gACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGAcgc  <  2:309845/151‑1 (MQ=255)
                                                                                                                                               gACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGAcgc  >  1:1561816/1‑151 (MQ=255)
                                                                                                                                               gACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGAcgc  <  1:444438/151‑1 (MQ=255)
                                                                                                                                               gACACACGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGAcgc  <  1:1554198/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTACATGTTGCTCGTCCAGCTCACCGCATGGGTTGCCGTCAAGATCGACACGCGTTGCGCCGGGTTTAACACCGTAAAGATAACGCCAGCTCGAAGTGTAGAGACGTAAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCATTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGC  >  NC_000913/1915149‑1915442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: