Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	43.1%	83.8 / 43.8	73	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/35);  new base T (31/0);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.07e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206289‑3206581                                                                                                                                                   |                                                                                                                                                     gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAg                                                                                                                                                   <  1:88669/150‑1 (MQ=255)    tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACa                                                                                                                                                >  1:796277/1‑150 (MQ=255)      tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCaaaaaa                                                                                                                                               >  2:503653/1‑144 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGaacaa                                                                                                                                              >  2:929975/1‑145 (MQ=255)        gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACaaaaa                                                                                                                                            >  1:561262/1‑148 (MQ=255)          aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACa                                                                                                                                          >  2:475770/1‑150 (MQ=255)                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑g                                                                                                                                  <  2:913541/150‑1 (MQ=255)                  gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGa                                                                                                                                <  1:687476/151‑1 (MQ=255)                    cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGt                                                                                                                              <  2:770953/151‑1 (MQ=255)                    cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGt                                                                                                                              <  1:529156/151‑1 (MQ=255)                    cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTCCAGACAAATACAAAAAcatg                                                                                                                                  >  1:223611/1‑145 (MQ=255)                       tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAATACAAAAAAA‑GGAggt                                                                                                                             >  2:766582/1‑147 (MQ=255)                        cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAAT‑TGAGTTGcc                                                                                                                          >  1:414197/1‑151 (MQ=255)                        cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGAGGTAGTTTGTGGCGGTGTATTTTTTCAGAGAAATACAAAAACT‑TGAg                                                                                                                               >  2:294438/1‑146 (MQ=255)                         ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCACAAAAAAACAAAAAAT‑gggggtggcc                                                                                                                         >  2:382982/1‑143 (MQ=255)                         ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAT‑GGAGtt                                                                                                                             >  1:275338/1‑147 (MQ=255)                         ccTGCTCTGGCGCAATATTCATTCTTATTCTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGATTGTGGCGGGGGGATTTTTCAAATAAATTAAAAAAAT‑gggggtagcc                                                                                                                         >  2:499470/1‑143 (MQ=255)                          cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAAT‑GGAGTTGGCat                                                                                                                        >  2:315301/1‑151 (MQ=255)                            gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatga                                                                                                                      <  2:704743/151‑1 (MQ=255)                            gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCat                                                                                                                        <  2:827038/149‑1 (MQ=255)                               ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATGTAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatgat                                                                                                                     <  1:587415/149‑1 (MQ=255)                                tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAT‑GGAGTTGCCatgat                                                                                                                     >  1:447034/1‑148 (MQ=255)                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGc                                                                                                                 <  1:126293/151‑1 (MQ=255)                                  gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAAAT‑TGAGGTGCCatggtggggg                                                                                                                >  1:475056/1‑147 (MQ=255)                                           tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAAAT‑GGAGGTTGCATGATTAGGGGAAGaaa                                                                                                         >  2:167882/1‑149 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAAAAAAAAT‑GGAGGTGGCCTTATGAGGGAAAGTAATAAACaa                                                                                                  >  1:57218/1‑149 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACCAAAAAAAAAAAA‑TGAGTGGCCCCCATGAGGGAAAAaaataaaaaa                                                                                                  >  1:970381/1‑145 (MQ=255)                                                    ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAGTTTTTGTAAACCAACACAAAAAAT‑AGGGTTGCCATGTTGGGAGGAAATAAAAaacaac                                                                                                 >  2:636605/1‑148 (MQ=255)                                                        cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                          <  1:890002/151‑1 (MQ=255)                                                        cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                          <  1:453616/151‑1 (MQ=255)                                                           gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAAT‑GGAGTTGGCATGGTGAGGGAAAGTAAAAAAAAAAAGGCAggg                                                                                         >  2:129898/1‑147 (MQ=255)                                                             tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                      <  1:595658/150‑1 (MQ=255)                                                             tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAAACAAAAAAT‑CGAGTTGCCATGATGAGCGGAAGAAAAAAACAACCAGCAGTGaa                                                                                       >  1:134918/1‑149 (MQ=255)                                                                   aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                               <  1:783073/151‑1 (MQ=255)                                                                    cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAAAAAAACT‑GGAGGTTCCCTGGTGAGGGGAAAGAATAAACAACAAGCCGTggaaaa                                                                                    >  1:815852/1‑140 (MQ=255)                                                                      aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAAAAAATACAAAAACT‑GGAGTTGTCATGATGGGCGAAAGTAATAAAGAAAAAGGAGAGAATaa                                                                                    >  1:292298/1‑143 (MQ=255)                                                                         tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGTATTTTTTCAGAAAACTAAAAAAAAT‑TGAGTTGGCATTATTAGGCCAAGAAATAAGAAAAAAGGCGGGAATAAGtt                                                                                 >  1:645651/1‑143 (MQ=255)                                                                          gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                        <  1:406325/151‑1 (MQ=255)                                                                             aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                     <  2:344345/151‑1 (MQ=255)                                                                              aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                    <  1:848613/151‑1 (MQ=255)                                                                              aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGATAAATTTTCCAGACAAATACAAAAACA‑GGAGTTTGCATTATGAGCGGAAGTAATAAGCAAAAAGGAGGGAAAAAAATGACagaaa                                                                         >  1:862242/1‑144 (MQ=255)                                                                               ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAAAAAAAACAAAAAAT‑GGAGGTTCCATGATGAGGGAAAAAAATAAACAAAAAGCAGGGAATAAAATGACAGAGAATTTCg                                                                   >  2:574142/1‑151 (MQ=255)                                                                                tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAAT‑GGAGGTGCCATGATGGGGGAAAGAAATAAAAAAAAAGCAGAGAATAAAATGAAAGAGATTGt                                                                     >  2:866715/1‑148 (MQ=255)                                                                                 aCATATTCACGGTGGCAAAAAAATAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                 <  1:295716/151‑1 (MQ=255)                                                                                   atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                              <  1:399192/151‑1 (MQ=255)                                                                                     atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                             <  2:574444/151‑1 (MQ=255)                                                                                       tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAAA‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAAAttt                                                           >  1:726828/1‑151 (MQ=255)                                                                                        cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACttt                                                           <  1:199059/150‑1 (MQ=255)                                                                                                 aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                                  <  1:934043/150‑1 (MQ=255)                                                                                                 aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑a                                                <  2:237539/151‑1 (MQ=255)                                                                                                  aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑At                                               <  2:909752/151‑1 (MQ=255)                                                                                                   aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAT‑GGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑Att                                              >  1:221485/1‑151 (MQ=255)                                                                                                   aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑Att                                              <  1:393530/151‑1 (MQ=255)                                                                                                     aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAA‑GGAGTTGCCCTGATTAGCGAAAGGAAAAAAAAACAGGCAGGGAAAAAATTGACAGAGATTGTCGCTAACTTTTCCCCCCTG‑Attt                                             >  1:79674/1‑150 (MQ=255)                                                                                                     aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAAAAAAAAAAAAAAAT‑GGGGTTTGCATGGTGAGCGGAAGTAATAAAAAAAAAGCAAGGAATAAATTGAAAAAGAATGTCGCTAAATTTTCCCCCCAG‑Atttt                                            >  2:376539/1‑150 (MQ=255)                                                                                                        ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAAAAAGTTGACAGGGATTGTCGCTAACTTTTCCGCCATG‑Attttt                                           >  1:702668/1‑147 (MQ=255)                                                                                                        ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAACAAAAAAT‑GGAGTTTCCATGATGAGCGGAAGTAAAAAACAACAGGGAGTGAAAAAATTGACAGAGATTTTCGCTAACTTTACCGCCCTG‑ATTTTTa                                          >  1:154364/1‑150 (MQ=255)                                                                                                               cccaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGACCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAAt                                   <  2:422244/148‑1 (MQ=255)                                                                                                                   tttttAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGTCAAATTAAAAAAAA‑GGAGTTGGCATGGTGAGGGGAAATAAAAAGCAACAGGCAGTGAATAAGGTGACAAGAAATGTTGCTAAATTTACCCCCATG‑GATTCTTCCCCaaatcc                                >  2:592056/1‑145 (MQ=255)                                                                                                                   tttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCt                               <  2:120330/150‑1 (MQ=255)                                                                                                                   tttttAGTGGTAGTTTGTGGCGGTAAATTTTTCCAAACAAAAACAAAAACT‑GGAGTTGCAATGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAAGTTAACAGAGATTGCCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCt                               <  2:659016/150‑1 (MQ=255)                                                                                                                   tttttAGTGGAAGTTTGTGGCGGTAAATTTTTCCAGAAAAATACAAAAACG‑GGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCAACCTTTACCGCCATG‑ATTTCTACCCGAATGCCt                               <  2:221932/150‑1 (MQ=255)                                                                                                                      ttAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAAATGGGGGTTGCATTGA‑GGGGGAAAGTAATAAAGAAAAAGCAGTGAATAAATTTGCAGAGAATGTCCCTAAATTTTCCGCCACGAATTTTTACCC‑AAAGCCtgc                             >  2:848186/1‑148 (MQ=255)                                                                                                                         gTGGTAGTTTGTGGCGGTAAATTTTCCAAGAAAAATACAAAAACT‑GGAGTTGCCATGATGAGCAAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCtgatga                          <  2:940581/149‑1 (MQ=255)                                                                                                                           ggTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGGGATTGTCGCTATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgt                       <  2:796440/150‑1 (MQ=255)                                                                                                                             tAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtgg                     <  1:475781/150‑1 (MQ=255)                                                                                                                                tttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtggt                    <  2:775656/148‑1 (MQ=255)                                                                                                                                 ttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACG‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                 <  1:667720/150‑1 (MQ=255)                                                                                                                                  tgtgGCGGTGAATTTTTTCCGACAAAAAAAAAAAAA‑GGGGGTTTCATGGAGAGCGAAAAGAATAAACAAAAAGCAGAGAAAAAAATGACAGAGATTGTCGCTAACTTTTCCGCCATG‑ATTTTTAACCGAATACCTCATGACGtggggg                  >  2:868758/1‑148 (MQ=255)                                                                                                                                      gCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt           >  1:114882/1‑151 (MQ=255)                                                                                                                                          tGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa        >  1:21912/1‑150 (MQ=255)                                                                                                                                               ttttCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc   <  2:927877/150‑1 (MQ=255)                                                                                                                                                tttCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt  <  2:133896/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206289‑3206581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑