Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,332,791	0	A	C	44.3%	97.9 / 17.5	61	V161G (GTG→GGG)	basS	sensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base A (29/5);  new base C (0/27);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 2.02e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGG  >  NC_000913/4332647‑4332938                                                                                                                                                 |                                                                                                                                                    tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCAccccc                                                                                                                                                <  2:244404/150‑1 (MQ=255) tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCAccccc                                                                                                                                                <  2:375214/150‑1 (MQ=255)       ttACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTg                                                                                                                                         >  1:415319/1‑151 (MQ=255)       ttACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTg                                                                                                                                         >  2:205144/1‑151 (MQ=255)       ttACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTg                                                                                                                                         >  1:741352/1‑151 (MQ=255)           cGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGt                                                                                                                                     >  1:939888/1‑151 (MQ=255)           cGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGt                                                                                                                                     >  1:730995/1‑151 (MQ=255)               agaaAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGc                                                                                                                                 >  1:75646/1‑151 (MQ=255)                      gccTGCCCGGCCCGCGCCAGTTGCAGCAGCTGGGAGCCGCTCCCCATCTTCTGATCAAGCCGTGCCACTAACGGTGCTCCATCATTGTGATGCTTTTTCGCCAGCATTCCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGt                                                                                                                          <  1:651847/149‑1 (MQ=255)                      gACTGTCCGCCCCGCGCAGGTGGCAGCAGTGGGGAGACGCTCTCCATCATCTGATCAACCCGTGCCACTACCGGTGCTACACAAATGTGAGGCTTTTTCGCCAGCAGTTCAAGATGAAACCCCCCCCCCGCCAGTGGCGTTCGCAGtt                                                                                                                             <  1:272272/148‑1 (MQ=255)                       attgcccGGCGCGCGCCGGTTGCGGCGGCGGGGAGCCGCTCCCAATCTTCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATTGGGTGCGTTTTCCCCAGCATTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTc                                                                                                                            <  1:43294/143‑1 (MQ=255)                            ccGGCACGCGCCAGTTGCAGCAGCGGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCCACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCCCCACCGCCAGTGGCGTTCGCAGTTCGTg                                                                                                                         <  1:540411/146‑1 (MQ=255)                              ggCACGCGCCAGTGGCAGCAGCGGGGAGCCTCTCTCCATCATCTGATCAAGCCGTGCCACTACCGGTGCTACATCAATGTGATGCTTTTCCGCCAGCAGTTCCAGATGAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGAc                                                                                                                   <  1:309357/150‑1 (MQ=255)                                   gcgcCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGc                                                                                                             >  2:727620/1‑151 (MQ=255)                                    cgcCAGTGGCGGCAGGGGGGGGACTCTCTCCATCATCTGATCAAGCCGTCCCAATAGCGGTGCTACAACAAGGTGATGCTTTTTCGCCAGCATTCCCAGATGAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGc                                                                                                             <  2:873507/150‑1 (MQ=255)                                      ccAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGt                                                                                                          >  2:788368/1‑151 (MQ=255)                                      ccAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGt                                                                                                          >  1:170571/1‑151 (MQ=255)                                      ccAGTGGCAGCAGCTGGGAGCCGCTCTCCACCATGTGATCACGCCGTCCCACTAACGGTGCTACATCAAGGTGTTGCTTTTCCCCCGGCAGTCCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGAc                                                                                                                   <  2:200687/142‑1 (MQ=255)                                      caaGTGGCAGCAGCGGGGAGCCCCTCCCCATCTTCTAATCAAGCCGTCCCACAAAGGGTCCTACATAAATGTGATGCTTTTTCGCCAGCAGTTCCAGATGAAAACCCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGc                                                                                                             <  1:192157/146‑1 (MQ=255)                                       cAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCgg                                                                                                           >  2:657159/1‑149 (MQ=255)                                           tGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaac                                                                                                      >  2:553991/1‑150 (MQ=255)                                                  gcTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCAAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTc                                                                                              <  1:651978/151‑1 (MQ=255)                                                   cTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCg                                                                                             >  2:591061/1‑151 (MQ=255)                                                    tgggggcccctctCCATCTTCTGATAAAGCCGTGCCACTACGGGTGCTACACCATTGTGATGTTTTTCCGCAAGAATTTCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaacaa                                                                                                    <  1:536188/134‑1 (MQ=255)                                                     gggagcccttctcCACCATTGGATCAAGCCGTCCCACAACGGGTGCTACATAAATTTGTTGCGTTTTCCCCACCGGTCCCAAAGGAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGtt                                                                                           <  1:630773/142‑1 (MQ=255)                                                           cGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATcc                                                                                       >  2:594713/1‑149 (MQ=255)                                                           cGCTCCCCATCATCTGATCAGGCCGTGCCACTACGGGTCCTACATCAATGTGATGCTTTTCCGCCAGCAGTCCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAg                                                                                     <  1:90186/151‑1 (MQ=255)                                                             ctctCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCg                                                                                   >  2:873982/1‑151 (MQ=255)                                                             ctccCCATCATCTAATCAAGCCGTCCCACTAACGGTGCTACATAAATGTGTTGTTTTTCCGCCAGCAGTCCCAGATGCAACCGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTAt                                                                                         <  2:477792/145‑1 (MQ=255)                                                                 ccATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCt                                                                               >  1:224211/1‑151 (MQ=255)                                                                       tcTAATCACGCGGTGCCACTAAGGTTGCTACATCAATGTGAGGCGTTTCCCCCGGCATTCCAAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAg                                                                                     <  2:790408/139‑1 (MQ=255)                                                                         taaTCAAGCCGTCCCACTACCGGTCTTACATCAATGTAATCCTTTTTCGCCAGCAGTTCAAGATGAAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTgg                                                                             <  1:149622/143‑1 (MQ=255)                                                                              aggCCGTGCCACTAACGGTGCTACATCAATGTGTTGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGc                                                                       <  1:333457/144‑1 (MQ=255)                                                                                gCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAc                                                                >  2:227284/1‑151 (MQ=255)                                                                                 ccGTGCCACTACCGGTGCTACAACATTGGGATGCGTTTCCGCCAGCGGTTCCAGATGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGa                                                                 <  1:141441/149‑1 (MQ=255)                                                                                 ccGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc                                                               >  1:247043/1‑151 (MQ=255)                                                                                 ccGTCCCACAACGGGTGCTACATAAATGTGATGCTTTTTCGCCAGCAGTTCCAGATGAAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACt                                                                   <  1:380346/147‑1 (MQ=255)                                                                                  cgcgCCACTAGCGGTGCTAAATAATTGTGATGTTTTTCCCCCAGCGGTCCCAGAGGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAca                                                               <  2:722674/147‑2 (MQ=255)                                                                                      ctaCTATCGGGCCTACATAAATGTGTTGTTTTTTGGCGAGCATTCCGAGAAGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGa                                                            <  1:576149/147‑1 (MQ=255)                                                                                      ccACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc                                                               >  1:260075/1‑146 (MQ=255)                                                                                       cACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc                                                               >  2:602513/1‑145 (MQ=255)                                                                                           agcGGTGCTAAATAAATGTGATGTTTTTTCGCCAGCATTCCGAGATCCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGt                                                        <  2:107515/146‑1 (MQ=255)                                                                                           aaCGGTGCTACATCAATGTGATGCTTTTTCGCCAGCAGTTCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTa                                                     <  1:950159/151‑1 (MQ=255)                                                                                           aaCGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGttt                                                      >  1:935843/1‑150 (MQ=255)                                                                                             cGGTGCTACATCAATGTGATGCGTTTTCCCCAGCAGTTCCAGATGCAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTaa                                                    <  1:357305/150‑1 (MQ=255)                                                                                              ggTGCTACATCAATGTGATGCTTTTCCGCCAGCAGTCCCAGATGAAAACGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAAc                                                   <  1:773713/150‑1 (MQ=255)                                                                                              ggTGCTACAAAAATGTGATGCGTTTTCGCCAGCAGTCCCAGAGGCAAACCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAAc                                                   <  1:226414/150‑1 (MQ=255)                                                                                               gTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGc                                                 >  2:139552/1‑151 (MQ=255)                                                                                                 gCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCg                                               >  1:833420/1‑151 (MQ=255)                                                                                                    aCATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaa                                             >  1:521904/1‑150 (MQ=255)                                                                                                        cAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAaccac                                        >  1:494927/1‑151 (MQ=255)                                                                                                         aaTGGGATGTTTTTTCGCCAGCGTTTCAAGATGAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAacca                                         <  2:946614/149‑1 (MQ=255)                                                                                                                tGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCg                                 >  1:534452/1‑150 (MQ=255)                                                                                                                   gTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAttt                             >  2:814644/1‑151 (MQ=255)                                                                                                                    ttttCGCCAGCAGTCCCAGATGCAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAttt                             <  1:392228/150‑1 (MQ=255)                                                                                                                     ttcccccgcaattcccAGATGCAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAttt                             <  2:354506/135‑1 (MQ=255)                                                                                                                          ccggcagTCCCAGATCAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGt                      <  2:730981/148‑1 (MQ=255)                                                                                                                           cagcagTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTg                     >  2:761938/1‑151 (MQ=255)                                                                                                                               agTTCCAGATGCAAACGCACCCCCGCCAGAGGCGTTCGCAGTTCGTTCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGTTTTCGATGGTGgcg                  >  2:377549/1‑150 (MQ=255)                                                                                                                                 tccaaGATGAAACCGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcgc                 <  1:160910/145‑1 (MQ=255)                                                                                                                                              cGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATgg  >  1:250227/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    TGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAATGTGATGCGTTTTCGCCAGCAGTTCCAGATGCAAACGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGG  >  NC_000913/4332647‑4332938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑