Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC41.1% 107.1 / 10.4 56T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (26/7);  new base C (0/23);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 7.85e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAT  >  NC_000913/1376358‑1376650
                                                                                                                                                      |                                                                                                                                                 
ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAccc                                                                                                                                                >  2:120904/1‑151 (MQ=255)
 tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAccc                                                                                                                                                <  1:825627/150‑1 (MQ=255)
  aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                               <  2:722767/150‑1 (MQ=255)
   ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                              <  1:527251/150‑1 (MQ=255)
   ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCg                                                                                                                                             >  1:465009/1‑151 (MQ=255)
   ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCg                                                                                                                                             <  1:875191/151‑1 (MQ=255)
     gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGcc                                                                                                                                           <  2:278474/151‑1 (MQ=255)
         aTGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATg                                                                                                                                       <  2:754918/151‑1 (MQ=255)
                 gccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGt                                                                                                                               >  2:430804/1‑151 (MQ=255)
                   cgcgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGtt                                                                                                                              >  2:582902/1‑150 (MQ=255)
                       tggCCTGGACGGCGCCCGAAAAAGGGTGAGTGATCCCCTGCGGGTACTGGAACGGGTCGCCGCGCGTCCCGGACCCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGAATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACa                                                                                                                           <  1:209655/147‑1 (MQ=255)
                       tAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtc                                                                                                                         >  1:281776/1‑151 (MQ=255)
                                 ggggacccaaaaaGGGGGAGTGTTCCCCGGGGCGTACTGAAACAGGTCCCCGCGCGTCCCGGAACGGGGGTTTACCCCGACCCGTAAACCCGCTACCAGAAAAAAAGGATCACCACCCCCCCCGATGCCCGCCGTTACATCGTCGaaaa                                                                                                                 <  2:423144/140‑1 (MQ=255)
                                     acgaaaaatTGGGGGGTGCTTCCCGGCGCTTACTGGAACAGGCCGCCGCCCGTCCCGGACCGGGGTTTTCTCTCAAACCGTAAACCCGTTATCGGCATCATTTAATAACCCCCCCCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATc                                                                                                            <  1:396704/145‑1 (MQ=255)
                                      ccaaaaaaGGGTGAGTGATCCCCTGCGCGTACGGGAACGGGTCGCCGCGCGTCCCGAACCCGGGGTTTATCCCAACCCTTTAACCCGCTATCAGGATCATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACg                                                                                                               <  1:86218/142‑1 (MQ=255)
                                          aaaggggggtgtgtTTCCCTGCCCTTCCGGGACCGGGTCCCCGCGCGTCCGGGACCCGTGTTGTTTCCCGACCTTTAAACCCCTTATCAGGTTAATATAATAAACCCCCCCCCCGTTCCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                        <  2:594559/139‑1 (MQ=255)
                                          aaaaGGGTGAGTGATTCCCGGCGGGTACGGAAACGGGTCCCCGCGCTTCCCGAACCGGGGGTGTACCCCGAACCGTTAACCCGCTATCAGGACAATATGACCAACCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                        <  2:156797/149‑1 (MQ=255)
                                           aaaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                      >  1:274860/1‑150 (MQ=255)
                                            aaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACAt                                                                                                    >  1:148840/1‑151 (MQ=255)
                                               ggTGAGTGTTCCCCTGCGGGTCGGGAAACGGGTCGCGGCGGGTCCGGGACCGGTGTTGTATCCCGACCCGTAAAACCGCTACAAGAATAATATGATCACCCCCCTGGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                                   <  2:542429/149‑1 (MQ=255)
                                                      gttccccTGCGCGTACGGGAACGGGTCGCCGCGTGTCCCGGAACCGGGGTGTATCTCAACCCGTAAACCCGCTATCAGGTTCATATGAAAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                                          <  2:410937/147‑1 (MQ=255)
                                                        ttCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                        >  2:659284/1‑151 (MQ=255)
                                                        tccccTGCGCGTACTGAACCGGGCCCCCGCGCGCCCGGAACCGTGGTTTTTCCCCAACCCTTAAACCCGTTATCAGGATCAAATAACAACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                           <  2:460481/146‑1 (MQ=255)
                                                           ccTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGc                                                                                     >  1:635486/1‑151 (MQ=255)
                                                                   tACGGGAACGGGTCGCCGCGCGTCCCGGAACGGTGGTTTATCTCGACCCGTAAACCCGCTATCAGGATAATATGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAt                                                                                  <  1:308734/146‑1 (MQ=255)
                                                                       gaaaCGGGTCGCCGCGCGTCCCGGACCGGTGGTGTATCTCGAACCGTTAACCCGCTAGCAGGTTCATATGATAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATca                                                                          <  2:694890/148‑1 (MQ=255)
                                                                        gAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                         >  1:669144/1‑150 (MQ=255)
                                                                             gctcccgcggcgccccGAAACGGTGGTTTATCCAGAACGTTTAACCCTCAATGAGAAAAATATGATCAACCCCCTGGCGGAAGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                                     <  2:253826/135‑1 (MQ=255)
                                                                              gcccccgcGCGCACCGGACCGGGGGTTTATCCCAACCCTTAAACCCGTTATCAGAATCATATAATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGaa                                                                    <  2:604637/145‑1 (MQ=255)
                                                                                cgccgcGCGTACCGAAACCGTGGTGTATCTCAAACCTTAAACCCGCTATCAGGATAATATAATCAACCCCCCCGCCGTGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                         <  2:664842/142‑1 (MQ=255)
                                                                                   cgcgcgTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                              >  1:303015/1‑150 (MQ=255)
                                                                                     cgcgTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                              >  1:215995/1‑148 (MQ=255)
                                                                                      gcgTCCCGGACCGGTGGTGTATCGCGACCCTTAAAACCGCTACCAGGATAATATAAACAACCCCCGCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaag                                                            <  2:271798/149‑1 (MQ=255)
                                                                                      gcgTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGCTTTCTATCACATGAATATCgaaga                                                           >  1:80577/1‑150 (MQ=255)
                                                                                       gcgtccGGAACCCGTGGTGTACCTCGAACCTTTAACCCGTTATCAGGATAATATGATAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaag                                                            <  2:171804/145‑1 (MQ=255)
                                                                                        gTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                           >  2:849299/1‑148 (MQ=255)
                                                                                           ccGGACCCGGGTTGTATCTCGACCCTTTAAACCGCTTCAAGGACCATATAACCAACCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAagaagaga                                                      <  1:163078/150‑3 (MQ=255)
                                                                                               aaCCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                 >  1:871671/1‑151 (MQ=255)
                                                                                                acccGTTGTGTTGCTCGAACTGTTAACCCGCTATAAGGATCATATGACCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                 <  2:268542/149‑1 (MQ=255)
                                                                                                 ccctttgtgtATTCCAACCTTTAAACCCGTTACCAGAATAATATGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                <  2:298733/145‑1 (MQ=255)
                                                                                                  ccGTGGTTTATCTCGACCCTTTAACCCTCTAACAGAATCATATTATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                <  1:311013/150‑1 (MQ=255)
                                                                                                    gtggtgTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcgc                                             >  2:80524/1‑151 (MQ=255)
                                                                                                        tgtATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                         >  1:316139/1‑151 (MQ=255)
                                                                                                             ctcGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGc                                    >  2:9934/1‑151 (MQ=255)
                                                                                                             cgcGAACGGTAAACCCGCTACCGGGATAATATGAACAACCCCCCCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGc                                    <  1:273237/149‑1 (MQ=255)
                                                                                                                gAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                 >  1:839673/1‑151 (MQ=255)
                                                                                                                 aaCCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                 >  2:801568/1‑150 (MQ=255)
                                                                                                                  aCCCTTAAACCCGCTATAAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCAt                                  <  1:375139/149‑1 (MQ=255)
                                                                                                                    cGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACa                              >  1:844772/1‑150 (MQ=255)
                                                                                                                    cGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACa                              >  1:667571/1‑150 (MQ=255)
                                                                                                                               cTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTg                   >  2:341263/1‑151 (MQ=255)
                                                                                                                                    cAGTATCATATAATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCt                 <  1:687646/149‑1 (MQ=255)
                                                                                                                                      ggATAATATAACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg               <  1:703335/149‑1 (MQ=255)
                                                                                                                                          cATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCa           >  2:172859/1‑149 (MQ=255)
                                                                                                                                            tatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgtg       >  1:504460/1‑151 (MQ=255)
                                                                                                                                                 tCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCatat  >  1:844948/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
TTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAT  >  NC_000913/1376358‑1376650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: