Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT41.9% 46.1 / 23.6 43intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/25);  new base T (18/0);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 1.64e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAA  >  NC_000913/3206289‑3206575
                                                                                                                                                  |                                                                                                                                             
gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGa                                                                                                                                           <  1:350938/151‑1 (MQ=255)
       gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGACAAATa                                                                                                                                     >  1:407241/1‑150 (MQ=255)
            aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaaa                                                                                                                               <  2:283993/151‑1 (MQ=255)
             cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATACaaaaa                                                                                                                               >  2:812318/1‑150 (MQ=255)
             cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAc                                                                                                                              <  2:238812/151‑1 (MQ=255)
                   cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATgg                                                                                                                           >  1:385275/1‑148 (MQ=255)
                    aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                       <  1:431388/151‑1 (MQ=255)
                               tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAGAAAAATAAAAAAAATGGAGGTGCCa                                                                                                                   >  2:558238/1‑144 (MQ=255)
                                ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                            <  1:242506/150‑1 (MQ=255)
                                ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc                                                                                                           <  2:493922/151‑1 (MQ=255)
                                 gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                          <  1:163637/151‑1 (MQ=255)
                                    cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                       <  2:701994/151‑1 (MQ=255)
                                      atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGt                                                                                                     <  1:310273/151‑1 (MQ=255)
                                          tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGAAGTGCGAAAgga                                                                                                    >  1:368917/1‑146 (MQ=255)
                                           cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGAAAGtaa                                                                                                   >  1:264994/1‑148 (MQ=255)
                                               cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGCCATGATGAGCGAAAGtaataa                                                                                                >  1:796343/1‑147 (MQ=255)
                                                    tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGAGGTGCCCTGATTAGGGAAAATAAAAAAGAACaag                                                                                        >  1:683589/1‑148 (MQ=255)
                                                      tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                      <  2:453105/150‑1 (MQ=255)
                                                       cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                    <  2:125921/151‑1 (MQ=255)
                                                         cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                  <  1:564603/151‑1 (MQ=255)
                                                              aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAg                                                                            <  2:64407/151‑1 (MQ=255)
                                                                  aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCGCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGa                                                                        <  2:569346/151‑1 (MQ=255)
                                                                          ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGGGTTGGCATGATGAGCGAAAGGAAAAAACAACAAGCAGAGAA‑AAAGTTGAAaga                                                                    >  2:882499/1‑147 (MQ=255)
                                                                                     ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAAAAAACAACAAGCAGGGAA‑TAAATTGACAGAGATTTTTGCTAACtt                                                     >  1:343194/1‑151 (MQ=255)
                                                                                       cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGACAAAAACAAAAAATTGAGTTGTCATGATGAGCGAAAGGAATAAAAAAAAAGCAGGGAA‑AAAAATGAAAGAGATTGTCGCTAACttt                                                    >  2:704302/1‑150 (MQ=255)
                                                                                        aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTAc                                                  <  1:673428/151‑1 (MQ=255)
                                                                                          ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGAGGTTCCATGATGAGCGAAAGTAAAAAACAAAAGGGAGTGAA‑AAAATTGACAGAGATTGTCGCTAAATTTAcc                                                 >  2:574945/1‑150 (MQ=255)
                                                                                                     atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCCTGATGAGGGAAAGAAATAAACAAAAAGCAGTGAA‑TAAAGTGAAAGAGATTGTTGCTAACTTTTACGCCCTGAttttt                                     >  1:622442/1‑149 (MQ=255)
                                                                                                       ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGAGTTGGCATGATGAGGGAAAGGAATAAACAAAAAGGAGGGAA‑TAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCCTGAttttt                                     >  2:66939/1‑147 (MQ=255)
                                                                                                              cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg                            <  1:228074/151‑1 (MQ=255)
                                                                                                                caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAGCAACAAGCAGTGAA‑TAAGATGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTACCCGAAAGcc                          >  1:583401/1‑151 (MQ=255)
                                                                                                                  tttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGGTGCCATGGTGAGCGGAAGGAATAAGCAAAAGGCAGTGGA‑TAAGTTGACAGAGATTGTCGCTAACTTTAACGCCATGATTTTTACCCGAAAGcc                          >  1:497122/1‑149 (MQ=255)
                                                                                                                   ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACCAAAACAAAAAATGGGGGTGCCATGATGAGGGGAAGGAATAAACAACAAGCAGGGGA‑AAAATTGAAAGAGAATGTCGCTAAATTTTCCCCCCTGGTTTCTTCCCCaaatgc                          >  2:477357/1‑144 (MQ=255)
                                                                                                                     ttAGTGGTAGTTGTTGGCGGTGATTTTTTCCAGACAAAGACAAAAACTGGAGTCACCATGATGAGCGAAAGTAATAACCAACAGGC‑GTGAATTAATTTGACAGATATTGTCGTTATTTTTACCGCCATGATTTCTACCCGAATGCCtgat                      <  1:67305/150‑1 (MQ=255)
                                                                                                                        tgggTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACGGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCtgatg                     <  1:585385/146‑1 (MQ=255)
                                                                                                                          ggTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAACTGGGGGTGCCATGATGAGGGAAAGGAAAAAACAAAAGGCAGCGGA‑TAAAATGAGAGAGAATTTTGCTAACTTTACCGCCACTATTTTTACCCCAACGcc                          >  2:388821/1‑141 (MQ=255)
                                                                                                                          ggTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                <  2:144980/151‑1 (MQ=255)
                                                                                                                           gTAGTTTGTGGCGGTAAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGGGCAAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg               <  2:625937/151‑1 (MQ=255)
                                                                                                                                  gtgGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGAGGAGCGAAAGTAATAAGCAACAGGCAGTAAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa         <  2:528633/150‑1 (MQ=255)
                                                                                                                                    ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa       <  2:186793/150‑1 (MQ=255)
                                                                                                                                       ggaaaaTTTTTCAAGAAAAATACAAAAACTGGGGTGCCCATGTTGAGCGAAAGTAATAAGCAACAGGCAGTAAA‑TAGGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa   <  1:146688/147‑1 (MQ=255)
                                                                                                                                       ggTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa   <  1:561698/151‑1 (MQ=255)
                                                                                                                                         tGAATTTTTCCAAACAAATAAAAAAACTGGATTTGCCATGATGAGCGAAAGTAATAGGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa  <  2:416846/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAA‑TAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAA  >  NC_000913/3206289‑3206575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑