Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,381,728	0	A	C	44.4%	83.1 / 11.1	54	G197G (GGT→GGG)	frdA	anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base A (25/5);  new base C (0/24);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 9.09e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGG  >  NC_000913/4381580‑4381878                                                                                                                                                     |                                                                                                                                                       cTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGAcc                                                                                                                                                      >  2:260276/1‑151 (MQ=255)        catcaGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCg                                                                                                                                               >  1:794215/1‑151 (MQ=255)         atcaGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGc                                                                                                                                              >  2:370761/1‑151 (MQ=255)            aGGATACCGGAACCTGGCAGACCGGTTGGGAGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGcc                                                                                                                                             >  2:174800/1‑149 (MQ=255)             ggATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAg                                                                                                                                           <  2:392656/150‑1 (MQ=255)               aTACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGt                                                                                                                                          >  2:739786/1‑149 (MQ=255)                      aaCCTGGCAGCCCGGTTGGGTGATACTGAACGAATTCCATGCCACGCGGCGGAACGCGGTGGCTTAGCGCCATCCCCATCCCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATaa                                                                                                                                  <  1:191700/150‑1 (MQ=255)                        ccgggCAGACGGGTGGGGTGATACTGAACGAATTCCATGTCCCGCAGCGGAACGCCGTGGCTTAGCGCCATCCCCATACCGTCCCCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAAc                                                                                                                                 <  1:621276/146‑1 (MQ=255)                          tGGCAGACCGGTTGGGTGATACTGAACCAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGCAACGATGCCGCCGCTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGAcc                                                                                                                             >  2:903756/1‑151 (MQ=255)                                         gTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATc                                                                                                              >  1:676670/1‑151 (MQ=255)                                         gTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATc                                                                                                              >  1:54205/1‑151 (MQ=255)                                            aTACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGc                                                                                                           >  1:394760/1‑151 (MQ=255)                                             tACTGAACGATTCCCATGTCCCGCAGCGGACCGCCGGGGTTTGGCGCAATCCCCACCCCGTCCCCGGAAACGATGCCGCCGTTGGTGTTGTAACAAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATc                                                                                                              <  2:64910/147‑1 (MQ=255)                                             tACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATCCCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTg                                                                                                            <  1:830703/149‑1 (MQ=255)                                               cTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCAcc                                                                                                        >  1:828053/1‑151 (MQ=255)                                                 gAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCAcc                                                                                                        >  1:125720/1‑149 (MQ=255)                                                   aCGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCg                                                                                                    >  1:598890/1‑151 (MQ=255)                                                   aCGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCg                                                                                                    >  1:28942/1‑151 (MQ=255)                                                      aatcccttttcccccAGCGGAACGCCGTGGTTAGGCACCATCCCCATCCCGTCCCCGGTAACGATCCCGCCGTTGGTGTTGAAAAGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAg                                                                                                      <  2:809160/132‑1 (MQ=255)                                                      aaTTCCATGTCACGCAGGGGAACGCCGTGGCTTAGCGCCATCCCCATCCCGTCCCCGGTAACGATGCCGCCGTTGGTGTTGTAACGAAAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGt                                                                                                   <  1:452646/149‑1 (MQ=255)                                                          ccATGTCCCGCAGCGGACCGCGGGGGCTTAGCGCAATCCCCATCCCGCCACCGGTAACGTTGCCGCCTTTGGTGTTGTAACGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCtt                                                                                              <  2:663308/150‑1 (MQ=255)                                                                cccGCAGCGGACCGCCGTGGCTTAGCCCCATCCCCATCCCGTCCCCGGTAACGAGGCCGCCGTTGGTTTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCcatca                                                                                        <  1:445029/148‑1 (MQ=255)                                                                     aGCGAAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTc                                                                                   >  2:838087/1‑150 (MQ=255)                                                                        ggAACGCCGTGGCTTAGCGCCATACCCATACCGTCCCCGGTAACGATGCCGCCGTTGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCAtt                                                                                <  1:160319/150‑1 (MQ=255)                                                                              cggTGGCTTAGCGCCATACCCATCCCGTCCCCGGTAACGATGCCGCCGTTGGTGTTGTACCGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCa                                                                         <  2:112701/149‑1 (MQ=255)                                                                              ccGTGGTTTAGCGCCAAACCCATCCCGTCACCGGAAACGATGCCGCCTTTGGTGTTGTAACGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCt                                                                             <  2:728826/147‑1 (MQ=255)                                                                                  gtcTTAGCGCCATCCCCATCCCGTCACCGGAACCGAGCCCGCCGTTGGTGTTGTAACAATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAg                                                                        <  2:585091/146‑1 (MQ=255)                                                                                  ggtttAGCGCCATCCCCATCCCGCCCCCGGTAACGATCCCCCCTTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCa                                                                         <  1:784589/144‑1 (MQ=255)                                                                                  ggCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGcc                                                                     >  2:443581/1‑151 (MQ=255)                                                                                     ttAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcgcg                                                                  >  2:400388/1‑151 (MQ=255)                                                                                      tAGCGCCATCCCCATCCCGTCCCCGGTAACGGTGCCGCCGTTGGTGTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcgc                                                                   <  1:565094/149‑1 (MQ=255)                                                                                          gcCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACAt                                                             >  2:140437/1‑151 (MQ=255)                                                                                             atacccataccGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGAc                                                          >  1:698917/1‑151 (MQ=255)                                                                                                   aaaccgccccgggTAACGGTCCGCCCGTTTGTGTTGGACCTATAAGCGGCCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGa                                                                 <  1:886313/127‑1 (MQ=255)                                                                                                     accGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCatcatc                                                   >  2:143712/1‑150 (MQ=255)                                                                                                      ccGTCCCCGGTAACGATGCCGCCGTTGGTTTTGAAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCaa                                                 <  2:334238/151‑1 (MQ=255)                                                                                                      ccGTCACCGGTACCGATGCCGCCGTTGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCaa                                                 <  1:479459/151‑1 (MQ=255)                                                                                                          cACCGGTACCGAGCCCGCGGTGGGTGTTGAAACGATAAACGCGACCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCa                                              <  2:343807/150‑1 (MQ=255)                                                                                                                    ggcgccgcgGTTGGTGTTGAAAGGAAAAACGCTCCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAg                                    <  2:612110/147‑1 (MQ=255)                                                                                                                        ccgccgTTGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCAc                                 <  2:652112/149‑1 (MQ=255)                                                                                                                        ccgccgTTGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCAc                                 <  1:807641/149‑1 (MQ=255)                                                                                                                             gTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTGGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATg                           >  2:886623/1‑150 (MQ=255)                                                                                                                             gTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGt                          >  1:35450/1‑151 (MQ=255)                                                                                                                              ttGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGaa                              <  2:394109/146‑1 (MQ=255)                                                                                                                              ttGGTGTTGTAACAAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGt                          <  1:645579/150‑1 (MQ=255)                                                                                                                                ggTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCCACCAGAATATCCAGCACGACATGTTc                        >  2:308503/1‑150 (MQ=255)                                                                                                                                    ttgaAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTc                     <  2:669476/149‑1 (MQ=255)                                                                                                                                    ttgaAACAAAAACCGCGCCCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTc                        <  2:335719/146‑1 (MQ=255)                                                                                                                                     tgtAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCaa                   <  1:322159/150‑1 (MQ=255)                                                                                                                                            aTAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCt             <  1:404722/149‑1 (MQ=255)                                                                                                                                              aagcgcgACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCt             <  2:30193/144‑1 (MQ=255)                                                                                                                                              aaaCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGAt         >  1:576015/1‑151 (MQ=255)                                                                                                                                                aCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCt       >  1:2046/1‑151 (MQ=255)                                                                                                                                                    gACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCgg   >  1:80508/1‑151 (MQ=255)                                                                                                                                                     aCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCggg  >  2:486514/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                       CTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAACGATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGG  >  NC_000913/4381580‑4381878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑