Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	293,574	A→G	D115D (GAT→GAC)	yagL ←	CP4‑6 prophage; DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	293,574	0	A	G	100.0%	258.4 / NA	63	D115D (GAT→GAC)	yagL	CP4‑6 prophage; DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/33);  total (30/33)

CTGACGAGCAATCAAAAAAGACAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGATCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCA  >  NC_000913/293427‑293718                                                                                                                                                    |                                                                                                                                                 cTGACGAGCAATCAAAAAAGACAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCt                                                                                                                                               >  2:497093/1‑151 (MQ=255)   gACGAGCAATCAAAAAAGACAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCt                                                                                                                                               <  2:172136/149‑1 (MQ=255)          aaTCAAAAAAGACAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGAc                                                                                                                                       >  2:529907/1‑150 (MQ=255)          aaTCAAAAAAGACAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACa                                                                                                                                      <  1:247393/151‑1 (MQ=255)          aaTCAAAAAAGACAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACa                                                                                                                                      <  2:432887/151‑1 (MQ=255)                    gACAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCt                                                                                                                            <  2:877429/151‑1 (MQ=255)                     aCAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTg                                                                                                                           <  2:481063/151‑1 (MQ=255)                      cAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGc                                                                                                                          >  2:901296/1‑151 (MQ=255)                      cAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGc                                                                                                                          <  1:712660/151‑1 (MQ=255)                         gCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCgg                                                                                                                       >  1:703825/1‑151 (MQ=255)                         gCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCgg                                                                                                                       <  2:394025/151‑1 (MQ=255)                                 cagcagAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGttcatttc                                                                                                               >  1:216729/1‑151 (MQ=255)                                 cagcagAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGttcattt                                                                                                                <  2:204689/150‑1 (MQ=255)                                                 ccTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGt                                                                                               >  1:772842/1‑151 (MQ=255)                                                   ttGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCt                                                                                             >  1:874404/1‑151 (MQ=255)                                                    tGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTg                                                                                            >  1:703122/1‑151 (MQ=255)                                                           ggATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTaa                                                                                       <  1:383101/149‑1 (MQ=255)                                                           ggATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAAc                                                                                     >  1:348265/1‑151 (MQ=255)                                                           ggATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAAc                                                                                     >  2:56645/1‑151 (MQ=255)                                                           ggATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAAc                                                                                     <  2:781666/151‑1 (MQ=255)                                                            gATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAAc                                                                                     <  1:468869/150‑1 (MQ=255)                                                               ggTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGtt                                                                                  <  1:404309/150‑1 (MQ=255)                                                                  aGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGct                                                                               >  2:916406/1‑150 (MQ=255)                                                                   gCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGctct                                                                             >  2:28417/1‑151 (MQ=255)                                                                      gTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGa                                                                           <  1:413373/150‑1 (MQ=255)                                                                             aaCATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACct                                                                   <  2:799237/151‑1 (MQ=255)                                                                             aaCATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACct                                                                   >  2:92464/1‑151 (MQ=255)                                                                                aTTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCtt                                                                <  2:884140/151‑1 (MQ=255)                                                                                   tCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTa                                                              <  2:500022/150‑1 (MQ=255)                                                                                       tttGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAatat                                                          <  1:118168/150‑1 (MQ=255)                                                                                        ttGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGATCTGAAACAACCTCTTTAATATc                                                         <  1:800454/150‑1 (MQ=255)                                                                                         tGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCt                                                        <  1:853237/150‑1 (MQ=255)                                                                                         tGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCt                                                        <  2:610878/150‑1 (MQ=255)                                                                                          gactgaaaaaaaaTAAAATATTCTGTAAGTCCTCCACAGATCTGCCAAGACAGCTAAGGTCTCAAATGACAAGCGTGTCCTGCCGGTTCATTTCGTGATAAATCCATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTAc                                                      <  2:73546/145‑1 (MQ=255)                                                                                            aGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCt                                                    >  2:855923/1‑151 (MQ=255)                                                                                            aGGCAAAAAAATAAAATATTCTGTAAGACCTCCACAGTTCTGCCAAGACAGCTTAGGTCGGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCCCTTTAATATCTACCt                                                    <  2:358713/151‑1 (MQ=255)                                                                                               cAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCt                                                 >  1:306272/1‑151 (MQ=255)                                                                                               cAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCt                                                 >  1:664321/1‑151 (MQ=255)                                                                                               cAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCt                                                 <  1:242186/151‑1 (MQ=255)                                                                                                 aaaaaaTAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCg                                               <  1:802449/151‑1 (MQ=255)                                                                                                 aaaaaaTAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCg                                               >  2:509047/1‑151 (MQ=255)                                                                                                  aaaaaTAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCg                                               >  1:20267/1‑150 (MQ=255)                                                                                                  aaaaaTAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGa                                              <  1:412009/151‑1 (MQ=255)                                                                                                    aaataaaataTTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATc                                            >  2:604686/1‑151 (MQ=255)                                                                                                        aaaataTTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAATCAACCTCTTTAATATCTACCTGCTCGATcaca                                         <  2:248482/150‑1 (MQ=255)                                                                                                           atatTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACAt                                     >  1:331419/1‑151 (MQ=255)                                                                                                             atTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGt                                   >  2:417322/1‑151 (MQ=255)                                                                                                             atTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGt                                   >  1:552813/1‑151 (MQ=255)                                                                                                               tCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGtt                                  <  1:338639/150‑1 (MQ=255)                                                                                                                  gTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCa                                <  1:777315/149‑1 (MQ=255)                                                                                                                   tAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAgg                              >  2:325208/1‑150 (MQ=255)                                                                                                                    aaGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTa                            <  2:748094/151‑1 (MQ=255)                                                                                                                     aGTCCTCCACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGt                             >  2:613157/1‑149 (MQ=255)                                                                                                                        cctccACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAat                        >  1:524734/1‑151 (MQ=255)                                                                                                                          tccACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAatat                      >  1:656742/1‑151 (MQ=255)                                                                                                                           ccACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATAtt                     >  1:280254/1‑151 (MQ=255)                                                                                                                           ccACAGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATAtt                     <  2:166251/151‑1 (MQ=255)                                                                                                                            cacaGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATAtt                     >  1:432304/1‑150 (MQ=255)                                                                                                                            cacaGTTCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATAtt                     >  2:894721/1‑150 (MQ=255)                                                                                                                                  tCTGCCAAGACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATATTAAAttt               <  2:909756/150‑1 (MQ=255)                                                                                                                                          gACAGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCatat      >  2:549335/1‑151 (MQ=255)                                                                                                                                             aGCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATAtctc   <  2:614313/151‑1 (MQ=255)                                                                                                                                              gCTTAGGTCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCa  <  1:349206/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CTGACGAGCAATCAAAAAAGACAAGCAACTTTCAGCAGAAGGCTCTATCCTTGAAGCTGGATGGTAGCTGTAGATAAACATTTCTTTTTGAAGGCAAAAAAATAAAATATTCTGTAAGTCCTCCACAGTTCTGCCAAGACAGCTTAGATCTGGAATGACAAGCGTGTCCTGCCGGTTCATTTCATGATGAATCAATTGTCTGAGTAAACGTTGCTCTGAAACAACCTCTTTAATATCTACCTGCTCGATCACAACATGTTCAGGTAGAATATTAAATTTTTGGCATATCTCA  >  NC_000913/293427‑293718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑