Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,725,329 C→A G140* (GGA→TGA)  kgtP ← alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,3290CA100.0% 247.7 / NA 62G140* (GGA→TGA) kgtPalpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/29);  total (33/29)

TCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGC  >  NC_000913/2725184‑2725472
                                                                                                                                                 |                                                                                                                                               
tCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCa                                                                                                                                             <  1:162337/150‑1 (MQ=255)
    tGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAAc                                                                                                                                           >  2:634685/1‑148 (MQ=255)
     ggTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGAt                                                                                                                                       <  1:622707/151‑1 (MQ=255)
             gtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCt                                                                                                                                <  1:602558/150‑1 (MQ=255)
             gtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTg                                                                                                                               <  2:740060/151‑1 (MQ=255)
             gtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTg                                                                                                                               >  1:255808/1‑151 (MQ=255)
              tAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTg                                                                                                                               >  2:163170/1‑150 (MQ=255)
                aaaCCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGaaa                                                                                                                            <  1:75660/151‑1 (MQ=255)
                 aaCCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGaaa                                                                                                                            >  2:398257/1‑150 (MQ=255)
                  aCCGCGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCTCTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATa                                                                                                                          <  2:3655/151‑1 (MQ=255)
                       gACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAAcga                                                                                                                     <  2:430286/151‑1 (MQ=255)
                        aCAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAAcg                                                                                                                      >  2:100723/1‑149 (MQ=255)
                            ccAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAAcgagcg                                                                                                                  >  2:617872/1‑149 (MQ=255)
                             cAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAAcgagcg                                                                                                                  >  1:247372/1‑148 (MQ=255)
                               gTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCgaga                                                                                                               >  2:6798/1‑149 (MQ=255)
                               gTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCgaga                                                                                                               >  1:224163/1‑149 (MQ=255)
                                 aGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGc                                                                                                            <  2:633394/150‑1 (MQ=255)
                                 aGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGc                                                                                                            <  2:71183/150‑1 (MQ=255)
                                 aGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCa                                                                                                           <  1:381981/151‑1 (MQ=255)
                                    gCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaata                                                                                                        <  2:580010/151‑1 (MQ=255)
                                       aGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaataat                                                                                                      <  1:709771/150‑1 (MQ=255)
                                       aGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaataa                                                                                                       <  2:170532/149‑1 (MQ=255)
                                         cAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGcc                                                                                                   >  1:590692/1‑151 (MQ=255)
                                          aGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCg                                                                                                  >  1:231201/1‑151 (MQ=255)
                                             tGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGa                                                                                                <  2:826252/150‑1 (MQ=255)
                                               tCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGcc                                                                                             >  1:491276/1‑151 (MQ=255)
                                                 cgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGccc                                                                                            >  2:252057/1‑150 (MQ=255)
                                                  gccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCAc                                                                                          >  2:451114/1‑151 (MQ=255)
                                                    cgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGt                                                                                        <  2:427756/151‑1 (MQ=255)
                                                          aCGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCtat                                                                                  <  1:778662/151‑1 (MQ=255)
                                                           cGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCtat                                                                                  >  1:193698/1‑150 (MQ=255)
                                                               acaTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGttt                                                                             >  1:194163/1‑151 (MQ=255)
                                                                caTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGttt                                                                             <  2:564844/150‑1 (MQ=255)
                                                                caTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTc                                                                            >  2:612718/1‑151 (MQ=255)
                                                                  tACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCAt                                                                          >  2:141994/1‑151 (MQ=255)
                                                                  tACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCAt                                                                          >  2:404570/1‑151 (MQ=255)
                                                                   aCTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCAt                                                                          <  1:555900/150‑1 (MQ=255)
                                                                    cTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATa                                                                         <  2:873196/150‑1 (MQ=255)
                                                                    cTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATa                                                                         <  1:592864/150‑1 (MQ=255)
                                                                             tgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGagg                                                               >  2:137238/1‑151 (MQ=255)
                                                                                gTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggc                                                              >  2:27059/1‑149 (MQ=255)
                                                                                      aCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGAt                                                       >  2:544385/1‑150 (MQ=255)
                                                                                         tttGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAAcc                                                   >  1:353029/1‑151 (MQ=255)
                                                                                           tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAAcc                                                   >  2:392052/1‑149 (MQ=255)
                                                                                            gcgcCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGc                                                <  1:642244/151‑1 (MQ=255)
                                                                                                   tCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg                                          >  1:729854/1‑150 (MQ=255)
                                                                                                       cGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAaca                                     <  1:91791/151‑1 (MQ=255)
                                                                                                        ggCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac                                    >  1:116607/1‑151 (MQ=255)
                                                                                                          cAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcat                                  >  2:104736/1‑151 (MQ=255)
                                                                                                           aaCTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatc                                 <  2:276648/151‑1 (MQ=255)
                                                                                                               tCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac                             >  2:176355/1‑151 (MQ=255)
                                                                                                                cACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATaca                            <  2:813248/151‑1 (MQ=255)
                                                                                                                 aCTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacac                           >  1:866478/1‑151 (MQ=255)
                                                                                                                   tCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac                         >  1:75881/1‑151 (MQ=255)
                                                                                                                    cATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacaca                          <  2:576780/149‑1 (MQ=255)
                                                                                                                       ataGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt                     >  1:368638/1‑151 (MQ=255)
                                                                                                                                gcTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc            >  2:67966/1‑151 (MQ=255)
                                                                                                                                   ggTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGa          <  2:859817/150‑1 (MQ=255)
                                                                                                                                     tGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAttt       >  1:49214/1‑151 (MQ=255)
                                                                                                                                        cATATTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAtttttt    <  2:592552/151‑1 (MQ=255)
                                                                                                                                           atTCTCAGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGc  <  2:255794/150‑1 (MQ=255)
                                                                                                                                             tctcAGCCAACAGATAATCCCTTAAAAAAACGGGCGAGAAGCAATAATGCCCGAGCCCACGTACCTATAGTTTCATAACCCGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGc  <  2:895597/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGC  >  NC_000913/2725184‑2725472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: