Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,643	Δ1 bp	coding (104/1299 nt)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,642	0	C	.	100.0%	207.4 / NA	43	coding (105/1299 nt)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/18);  total (25/18)

CAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTC  >  NC_000913/2725497‑2725783                                                                                                                                                  |                                                                                                                                              cAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCg                                                                                                                                         >  2:91513/1‑151 (MQ=255)  aaaTAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGa                                                                                                                                        <  2:704192/151‑1 (MQ=255)   aaTAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGAc                                                                                                                                       >  1:894255/1‑151 (MQ=255)       gCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGAcc                                                                                                                                      >  2:185527/1‑148 (MQ=255)         cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGAtt                                                                                                                                 >  2:205209/1‑151 (MQ=255)             cGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCt                                                                                                                             <  2:121140/151‑1 (MQ=255)              gCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTg                                                                                                                            >  1:276215/1‑151 (MQ=255)                 tATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGa                                                                                                                           >  2:742860/1‑149 (MQ=255)                    tGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAagag                                                                                                                       <  2:696704/150‑1 (MQ=255)                         gcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGccccc                                                                                                                 >  1:419546/1‑151 (MQ=255)                             cAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCcaca                                                                                                              >  2:52123/1‑150 (MQ=255)                              aGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAAt                                                                                                            <  1:967244/151‑1 (MQ=255)                                          gcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcg                                                                                                >  1:24776/1‑151 (MQ=255)                                               aaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgac                                                                                           <  1:166141/151‑1 (MQ=255)                                                 cacCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgacg                                                                                          >  2:743452/1‑150 (MQ=255)                                                  acCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgacg                                                                                          >  2:576882/1‑149 (MQ=255)                                                  acCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgacg                                                                                          >  1:919645/1‑149 (MQ=255)                                                    ctgctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGt                                                                                       >  1:995515/1‑150 (MQ=255)                                                      gctgTTTGTAGTAGTTGGGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAGCGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGTCCCCCACAACCGCCCAAATGCGGCGACGAGTAt                                                                                     <  2:586270/150‑1 (MQ=255)                                                                gtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTc                                                                          >  2:148475/1‑151 (MQ=255)                                                                gtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTc                                                                          >  2:366453/1‑151 (MQ=255)                                                                 tagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTc                                                                          >  1:78036/1‑150 (MQ=255)                                                                     tGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGttt                                                                     >  1:631964/1‑151 (MQ=255)                                                                       aGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGc                                                                   <  2:542764/151‑1 (MQ=255)                                                                              gtgtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGc                                                            >  2:529960/1‑151 (MQ=255)                                                                                     cGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTAc                                                      >  1:501448/1‑150 (MQ=255)                                                                                          gggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACt                                                <  1:461350/151‑1 (MQ=255)                                                                                                 agaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgcc                                         <  1:88599/151‑1 (MQ=255)                                                                                                      tgGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgcc                                    >  2:46911/1‑151 (MQ=255)                                                                                                       gggCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgcc                                    >  2:87934/1‑150 (MQ=255)                                                                                                        ggCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgccat                                  <  1:720750/151‑1 (MQ=255)                                                                                                                 gagTGAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc                         >  2:270197/1‑151 (MQ=255)                                                                                                                     gAACAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGc                      <  1:573403/150‑1 (MQ=255)                                                                                                                      aaCAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCg                    >  1:418967/1‑151 (MQ=255)                                                                                                                      aaCAGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCg                    <  2:671104/151‑1 (MQ=255)                                                                                                                         aGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTCCCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTa                  <  2:400106/150‑1 (MQ=255)                                                                                                                         aGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTaa                 <  1:742877/151‑1 (MQ=255)                                                                                                                         aGAACGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTa                  >  1:97430/1‑150 (MQ=255)                                                                                                                           aaCGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATc               <  1:599456/151‑1 (MQ=255)                                                                                                                            aCGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATcc              <  1:675205/151‑1 (MQ=255)                                                                                                                             cGAGTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATcc              <  2:51551/150‑1 (MQ=255)                                                                                                                                gTAGACATAGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGAt           >  1:46809/1‑150 (MQ=255)                                                                                                                                        aGAAATCGAA‑CACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTgtc  <  1:654089/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                              CAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTC  >  NC_000913/2725497‑2725783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑